Porcine circovirus type 2 (PCV2) infection decreases the efficacy of an attenuated classical swine fever virus (CSFV) vaccine
<p>Abstract</p> <p>The Lapinized Philippines Coronel (LPC) vaccine, an attenuated strain of classical swine fever virus (CSFV), is an important tool for the prevention and control of CSFV infection and is widely and routinely used in most CSF endemic areas, including Taiwan. The ai...
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| Format: | Article |
| Language: | English |
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BMC
2011-12-01
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| Series: | Veterinary Research |
| Online Access: | http://www.veterinaryresearch.org/content/42/1/115 |
| _version_ | 1818379717892374528 |
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| author | Huang Yu-Liang Pang Victor Lin Chun-Ming Tsai Yi-Chieh Chia Mi-Yuan Deng Ming-Chung Chang Chia-Yi Jeng Chian-Ren |
| author_facet | Huang Yu-Liang Pang Victor Lin Chun-Ming Tsai Yi-Chieh Chia Mi-Yuan Deng Ming-Chung Chang Chia-Yi Jeng Chian-Ren |
| author_sort | Huang Yu-Liang |
| collection | DOAJ |
| description | <p>Abstract</p> <p>The Lapinized Philippines Coronel (LPC) vaccine, an attenuated strain of classical swine fever virus (CSFV), is an important tool for the prevention and control of CSFV infection and is widely and routinely used in most CSF endemic areas, including Taiwan. The aim of this study was to investigate whether PCV2 infection affects the efficacy of the LPC vaccine. Eighteen 6-week-old, cesarean-derived and colostrum-deprived (CDCD), crossbred pigs were randomly assigned to four groups. A total of 10<sup>5.3 </sup>TCID<sub>50 </sub>of PCV2 was experimentally inoculated into pigs through both intranasal and intramuscular routes at 0 days post-inoculation (dpi) followed by LPC vaccination 12 days later. All the animals were challenged with wild-type CSFV (ALD stain) at 27 dpi and euthanized at 45 dpi. Following CSFV challenge, the LPC-vaccinated pigs pre-inoculated with PCV2 showed transient fever, viremia, and viral shedding in the saliva and feces. The number of IgM<sup>+</sup>, CD4<sup>+</sup>CD8<sup>-</sup>CD25<sup>+</sup>, CD4<sup>+</sup>CD8<sup>+</sup>CD25<sup>+</sup>, and CD4<sup>-</sup>CD8<sup>+</sup>CD25<sup>+ </sup>lymphocyte subsets and the level of neutralizing antibodies against CSFV were significantly higher in the animals with LPC vaccination alone than in the pigs with PCV2 inoculation/LPC vaccination. In addition, PCV2-derived inhibition of the CSFV-specific cell proliferative response of peripheral blood mononuclear cells (PBMCs) was demonstrated in an ex vivo experiment. These findings indicate that PCV2 infection decreases the efficacy of the LPC vaccine. This PCV2-derived interference may not only allow the invasion of wild-type CSFV in pig farms but also increases the difficulty of CSF prevention and control in CSF endemic areas.</p> |
| first_indexed | 2024-12-14T02:07:14Z |
| format | Article |
| id | doaj.art-903a4a9113534ed09ec43172a926879f |
| institution | Directory Open Access Journal |
| issn | 0928-4249 1297-9716 |
| language | English |
| last_indexed | 2024-12-14T02:07:14Z |
| publishDate | 2011-12-01 |
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| series | Veterinary Research |
| spelling | doaj.art-903a4a9113534ed09ec43172a926879f2022-12-21T23:20:52ZengBMCVeterinary Research0928-42491297-97162011-12-0142111510.1186/1297-9716-42-115Porcine circovirus type 2 (PCV2) infection decreases the efficacy of an attenuated classical swine fever virus (CSFV) vaccineHuang Yu-LiangPang VictorLin Chun-MingTsai Yi-ChiehChia Mi-YuanDeng Ming-ChungChang Chia-YiJeng Chian-Ren<p>Abstract</p> <p>The Lapinized Philippines Coronel (LPC) vaccine, an attenuated strain of classical swine fever virus (CSFV), is an important tool for the prevention and control of CSFV infection and is widely and routinely used in most CSF endemic areas, including Taiwan. The aim of this study was to investigate whether PCV2 infection affects the efficacy of the LPC vaccine. Eighteen 6-week-old, cesarean-derived and colostrum-deprived (CDCD), crossbred pigs were randomly assigned to four groups. A total of 10<sup>5.3 </sup>TCID<sub>50 </sub>of PCV2 was experimentally inoculated into pigs through both intranasal and intramuscular routes at 0 days post-inoculation (dpi) followed by LPC vaccination 12 days later. All the animals were challenged with wild-type CSFV (ALD stain) at 27 dpi and euthanized at 45 dpi. Following CSFV challenge, the LPC-vaccinated pigs pre-inoculated with PCV2 showed transient fever, viremia, and viral shedding in the saliva and feces. The number of IgM<sup>+</sup>, CD4<sup>+</sup>CD8<sup>-</sup>CD25<sup>+</sup>, CD4<sup>+</sup>CD8<sup>+</sup>CD25<sup>+</sup>, and CD4<sup>-</sup>CD8<sup>+</sup>CD25<sup>+ </sup>lymphocyte subsets and the level of neutralizing antibodies against CSFV were significantly higher in the animals with LPC vaccination alone than in the pigs with PCV2 inoculation/LPC vaccination. In addition, PCV2-derived inhibition of the CSFV-specific cell proliferative response of peripheral blood mononuclear cells (PBMCs) was demonstrated in an ex vivo experiment. These findings indicate that PCV2 infection decreases the efficacy of the LPC vaccine. This PCV2-derived interference may not only allow the invasion of wild-type CSFV in pig farms but also increases the difficulty of CSF prevention and control in CSF endemic areas.</p>http://www.veterinaryresearch.org/content/42/1/115 |
| spellingShingle | Huang Yu-Liang Pang Victor Lin Chun-Ming Tsai Yi-Chieh Chia Mi-Yuan Deng Ming-Chung Chang Chia-Yi Jeng Chian-Ren Porcine circovirus type 2 (PCV2) infection decreases the efficacy of an attenuated classical swine fever virus (CSFV) vaccine Veterinary Research |
| title | Porcine circovirus type 2 (PCV2) infection decreases the efficacy of an attenuated classical swine fever virus (CSFV) vaccine |
| title_full | Porcine circovirus type 2 (PCV2) infection decreases the efficacy of an attenuated classical swine fever virus (CSFV) vaccine |
| title_fullStr | Porcine circovirus type 2 (PCV2) infection decreases the efficacy of an attenuated classical swine fever virus (CSFV) vaccine |
| title_full_unstemmed | Porcine circovirus type 2 (PCV2) infection decreases the efficacy of an attenuated classical swine fever virus (CSFV) vaccine |
| title_short | Porcine circovirus type 2 (PCV2) infection decreases the efficacy of an attenuated classical swine fever virus (CSFV) vaccine |
| title_sort | porcine circovirus type 2 pcv2 infection decreases the efficacy of an attenuated classical swine fever virus csfv vaccine |
| url | http://www.veterinaryresearch.org/content/42/1/115 |
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