Chemical Scalpel: An Experimental Collagenase-Based Treatment for Peritoneal Adhesions
(1) Background: Abdominal adhesions are a common disease appearing after any type of abdominal surgery and may prolong surgical time and cause intestinal obstruction, infertility, or chronic pain. We propose the use of intraperitoneal collagenase to perform chemical adhesiolysis based on the pathoph...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/11/8/1159 |
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| author | Javier Barambio Mariano García-Arranz Pedro Villarejo Campos Juan Felipe Vélez Pinto Luz Vega Clemente Soledad García Gómez-Heras Héctor Guadalajara Damián García-Olmo |
| author_facet | Javier Barambio Mariano García-Arranz Pedro Villarejo Campos Juan Felipe Vélez Pinto Luz Vega Clemente Soledad García Gómez-Heras Héctor Guadalajara Damián García-Olmo |
| author_sort | Javier Barambio |
| collection | DOAJ |
| description | (1) Background: Abdominal adhesions are a common disease appearing after any type of abdominal surgery and may prolong surgical time and cause intestinal obstruction, infertility, or chronic pain. We propose the use of intraperitoneal collagenase to perform chemical adhesiolysis based on the pathophysiology and histology of adhesions. (2) Methods: We generated an adhesion model with intraperitoneal polypropylene meshes. Four months later, we evaluated the efficacy of the treatment in blinded form, i.e., 0.05% collagenase vs. placebo at 37 °C for 20 min. Protocol 1: Ten rats with ten mesh fragments, in which an attempt was made to remove the maximum number of meshes in a 5-min period. Protocol 2: Six rats with four mesh fragments in the sides of the abdominal cavity in which adhesiolysis was performed using a device that measures burst pressure. (3) Results: Protocol 1: 42% efficacy in the collagenase group versus 8% in the control group (<i>p</i> < 0.013). Protocol 2: 188.25 mmHg (SD 69.65) in the collagenase group vs. 325.76 mmHg (SD 50.25) in the control group (<i>p</i> < 0.001). (4) Conclusions: Collagenase allows for the safe and effective chemical adhesiolysis in this experimental model of adhesions. |
| first_indexed | 2024-03-09T04:41:08Z |
| format | Article |
| id | doaj.art-903bfef8b0eb43c1a7d9448da90043d8 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T04:41:08Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-903bfef8b0eb43c1a7d9448da90043d82023-12-03T13:21:00ZengMDPI AGBiology2079-77372022-08-01118115910.3390/biology11081159Chemical Scalpel: An Experimental Collagenase-Based Treatment for Peritoneal AdhesionsJavier Barambio0Mariano García-Arranz1Pedro Villarejo Campos2Juan Felipe Vélez Pinto3Luz Vega Clemente4Soledad García Gómez-Heras5Héctor Guadalajara6Damián García-Olmo7Fundación Jiménez Díaz University Hospital, 28033 Madrid, SpainFundación Jiménez Díaz University Hospital, 28033 Madrid, SpainFundación Jiménez Díaz University Hospital, 28033 Madrid, SpainFundación Jiménez Díaz University Hospital, 28033 Madrid, SpainFundación Jiménez Díaz University Hospital, 28033 Madrid, SpainDepartment of Human Histology, Faculty of Health Sciences, Rey Juan Carlos University, 28922 Madrid, SpainFundación Jiménez Díaz University Hospital, 28033 Madrid, SpainFundación Jiménez Díaz University Hospital, 28033 Madrid, Spain(1) Background: Abdominal adhesions are a common disease appearing after any type of abdominal surgery and may prolong surgical time and cause intestinal obstruction, infertility, or chronic pain. We propose the use of intraperitoneal collagenase to perform chemical adhesiolysis based on the pathophysiology and histology of adhesions. (2) Methods: We generated an adhesion model with intraperitoneal polypropylene meshes. Four months later, we evaluated the efficacy of the treatment in blinded form, i.e., 0.05% collagenase vs. placebo at 37 °C for 20 min. Protocol 1: Ten rats with ten mesh fragments, in which an attempt was made to remove the maximum number of meshes in a 5-min period. Protocol 2: Six rats with four mesh fragments in the sides of the abdominal cavity in which adhesiolysis was performed using a device that measures burst pressure. (3) Results: Protocol 1: 42% efficacy in the collagenase group versus 8% in the control group (<i>p</i> < 0.013). Protocol 2: 188.25 mmHg (SD 69.65) in the collagenase group vs. 325.76 mmHg (SD 50.25) in the control group (<i>p</i> < 0.001). (4) Conclusions: Collagenase allows for the safe and effective chemical adhesiolysis in this experimental model of adhesions.https://www.mdpi.com/2079-7737/11/8/1159collagenaseadherencesenzymatic adhesiolysisperitoneum treatmentsperitoneal surgeryexperimental model |
| spellingShingle | Javier Barambio Mariano García-Arranz Pedro Villarejo Campos Juan Felipe Vélez Pinto Luz Vega Clemente Soledad García Gómez-Heras Héctor Guadalajara Damián García-Olmo Chemical Scalpel: An Experimental Collagenase-Based Treatment for Peritoneal Adhesions Biology collagenase adherences enzymatic adhesiolysis peritoneum treatments peritoneal surgery experimental model |
| title | Chemical Scalpel: An Experimental Collagenase-Based Treatment for Peritoneal Adhesions |
| title_full | Chemical Scalpel: An Experimental Collagenase-Based Treatment for Peritoneal Adhesions |
| title_fullStr | Chemical Scalpel: An Experimental Collagenase-Based Treatment for Peritoneal Adhesions |
| title_full_unstemmed | Chemical Scalpel: An Experimental Collagenase-Based Treatment for Peritoneal Adhesions |
| title_short | Chemical Scalpel: An Experimental Collagenase-Based Treatment for Peritoneal Adhesions |
| title_sort | chemical scalpel an experimental collagenase based treatment for peritoneal adhesions |
| topic | collagenase adherences enzymatic adhesiolysis peritoneum treatments peritoneal surgery experimental model |
| url | https://www.mdpi.com/2079-7737/11/8/1159 |
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