Phenotypic and Genotypic Investigation of Carbapenem-Resistant <i>Acinetobacter baumannii</i> in Maharaj Nakhon Si Thammarat Hospital, Thailand
(1) Background: <i>Acinetobacter baumannii</i> is well known as a causative agent of severe hospital-acquired infections, especially in intensive care units. The present study characterised the genetic traits of biofilm-forming carbapenem-resistant <i>A. baumannii</i> (CRAB)...
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MDPI AG
2023-03-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/3/580 |
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| author | Sirijan Santajit Phuangthip Bhoopong Thida Kong-Ngoen Witawat Tunyong Dararat Horpet Wanfudhla Paehoh-ele Tasneem Zahedeng Pornpan Pumirat Nitat Sookrung Woranich Hinthong Nitaya Indrawattana |
| author_facet | Sirijan Santajit Phuangthip Bhoopong Thida Kong-Ngoen Witawat Tunyong Dararat Horpet Wanfudhla Paehoh-ele Tasneem Zahedeng Pornpan Pumirat Nitat Sookrung Woranich Hinthong Nitaya Indrawattana |
| author_sort | Sirijan Santajit |
| collection | DOAJ |
| description | (1) Background: <i>Acinetobacter baumannii</i> is well known as a causative agent of severe hospital-acquired infections, especially in intensive care units. The present study characterised the genetic traits of biofilm-forming carbapenem-resistant <i>A. baumannii</i> (CRAB) clinical isolates. Additionally, this study determined the prevalence of biofilm-producing <i>A. baumannii</i> isolates from a tertiary care hospital and investigated the association of biofilms with the distribution of biofilm-related and antibiotic resistance-associated genotypes. (2) Methods: The 995 non-duplicate <i>A. baumannii</i> isolates were identified, and their susceptibilities to different antibiotics were determined using the disk diffusion method. Using the modified microtiter plate assay, the CRAB isolates were investigated for their biofilm formation ability. Hemolysin and protease activities were determined. CRABs were subjected to polymerase chain reaction (PCR) assays targeting <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>IMP</sub>, <i>bla</i><sub>OXA-23-like</sub>, <i>bla</i><sub>OXA-24-like</sub>, <i>bla</i><sub>OXA-51-like</sub>, <i>csu</i>E and <i>pga</i>B genes. Individual CRAB isolates were identified for their DNA fingerprint by repetitive element sequence-based (REP)-PCR. (3) Results: Among all <i>A. baumannii</i> isolates, 172 CRABs were identified. The major antibiotic resistance gene among the CRAB isolates was <i>bla</i><sub>OXA-51-like</sub> (100%). Ninety-nine isolates (57.56%) were biofilm producers. The most prevalent biofilm gene was <i>pga</i>B (79.65%), followed by <i>csu</i>E (76.74%). Evidence of virulence phenotypes revealed that all CRAB exhibited proteolytic activity; however, only four isolates (2.33%) were positive for the hemolytic-producing phenotype. REP-PCR showed that 172 CRAB isolates can be divided into 36-DNA fingerprint patterns. (4) Conclusions: The predominance of biofilm-producing CRAB isolates identified in this study is concerning. The characterisation of risk factors could aid in controlling the continual selection and spreading of the <i>A. baumannii</i> phenotype in hospitals, thereby improving patient care quality. |
| first_indexed | 2024-03-11T07:01:42Z |
| format | Article |
| id | doaj.art-903e3696390c49d2bb5b6fa862511c08 |
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| language | English |
| last_indexed | 2024-03-11T07:01:42Z |
| publishDate | 2023-03-01 |
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| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-903e3696390c49d2bb5b6fa862511c082023-11-17T09:15:03ZengMDPI AGAntibiotics2079-63822023-03-0112358010.3390/antibiotics12030580Phenotypic and Genotypic Investigation of Carbapenem-Resistant <i>Acinetobacter baumannii</i> in Maharaj Nakhon Si Thammarat Hospital, ThailandSirijan Santajit0Phuangthip Bhoopong1Thida Kong-Ngoen2Witawat Tunyong3Dararat Horpet4Wanfudhla Paehoh-ele5Tasneem Zahedeng6Pornpan Pumirat7Nitat Sookrung8Woranich Hinthong9Nitaya Indrawattana10Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, ThailandDepartment of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, ThailandDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, ThailandDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, ThailandDepartment of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, ThailandDepartment of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, ThailandDepartment of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, ThailandDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, ThailandSiriraj Center of Research Excellence in Allergy and Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandPrincess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok 10210, ThailandDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand(1) Background: <i>Acinetobacter baumannii</i> is well known as a causative agent of severe hospital-acquired infections, especially in intensive care units. The present study characterised the genetic traits of biofilm-forming carbapenem-resistant <i>A. baumannii</i> (CRAB) clinical isolates. Additionally, this study determined the prevalence of biofilm-producing <i>A. baumannii</i> isolates from a tertiary care hospital and investigated the association of biofilms with the distribution of biofilm-related and antibiotic resistance-associated genotypes. (2) Methods: The 995 non-duplicate <i>A. baumannii</i> isolates were identified, and their susceptibilities to different antibiotics were determined using the disk diffusion method. Using the modified microtiter plate assay, the CRAB isolates were investigated for their biofilm formation ability. Hemolysin and protease activities were determined. CRABs were subjected to polymerase chain reaction (PCR) assays targeting <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>IMP</sub>, <i>bla</i><sub>OXA-23-like</sub>, <i>bla</i><sub>OXA-24-like</sub>, <i>bla</i><sub>OXA-51-like</sub>, <i>csu</i>E and <i>pga</i>B genes. Individual CRAB isolates were identified for their DNA fingerprint by repetitive element sequence-based (REP)-PCR. (3) Results: Among all <i>A. baumannii</i> isolates, 172 CRABs were identified. The major antibiotic resistance gene among the CRAB isolates was <i>bla</i><sub>OXA-51-like</sub> (100%). Ninety-nine isolates (57.56%) were biofilm producers. The most prevalent biofilm gene was <i>pga</i>B (79.65%), followed by <i>csu</i>E (76.74%). Evidence of virulence phenotypes revealed that all CRAB exhibited proteolytic activity; however, only four isolates (2.33%) were positive for the hemolytic-producing phenotype. REP-PCR showed that 172 CRAB isolates can be divided into 36-DNA fingerprint patterns. (4) Conclusions: The predominance of biofilm-producing CRAB isolates identified in this study is concerning. The characterisation of risk factors could aid in controlling the continual selection and spreading of the <i>A. baumannii</i> phenotype in hospitals, thereby improving patient care quality.https://www.mdpi.com/2079-6382/12/3/580biofilmsCRABmultidrug resistancenosocomial infectionsREP-PCR |
| spellingShingle | Sirijan Santajit Phuangthip Bhoopong Thida Kong-Ngoen Witawat Tunyong Dararat Horpet Wanfudhla Paehoh-ele Tasneem Zahedeng Pornpan Pumirat Nitat Sookrung Woranich Hinthong Nitaya Indrawattana Phenotypic and Genotypic Investigation of Carbapenem-Resistant <i>Acinetobacter baumannii</i> in Maharaj Nakhon Si Thammarat Hospital, Thailand Antibiotics biofilms CRAB multidrug resistance nosocomial infections REP-PCR |
| title | Phenotypic and Genotypic Investigation of Carbapenem-Resistant <i>Acinetobacter baumannii</i> in Maharaj Nakhon Si Thammarat Hospital, Thailand |
| title_full | Phenotypic and Genotypic Investigation of Carbapenem-Resistant <i>Acinetobacter baumannii</i> in Maharaj Nakhon Si Thammarat Hospital, Thailand |
| title_fullStr | Phenotypic and Genotypic Investigation of Carbapenem-Resistant <i>Acinetobacter baumannii</i> in Maharaj Nakhon Si Thammarat Hospital, Thailand |
| title_full_unstemmed | Phenotypic and Genotypic Investigation of Carbapenem-Resistant <i>Acinetobacter baumannii</i> in Maharaj Nakhon Si Thammarat Hospital, Thailand |
| title_short | Phenotypic and Genotypic Investigation of Carbapenem-Resistant <i>Acinetobacter baumannii</i> in Maharaj Nakhon Si Thammarat Hospital, Thailand |
| title_sort | phenotypic and genotypic investigation of carbapenem resistant i acinetobacter baumannii i in maharaj nakhon si thammarat hospital thailand |
| topic | biofilms CRAB multidrug resistance nosocomial infections REP-PCR |
| url | https://www.mdpi.com/2079-6382/12/3/580 |
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