Critical Torque for Kink Formation in Double-Stranded DNA
We measure the bending energy of double-stranded DNA in the nonlinear (sharply bent) regime. The measurements are obtained from the melting curves of stressed DNA ring molecules. The nonlinear elastic behavior is captured by a single parameter: the critical torque τ_{c} at which the molecule develop...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Physical Society
2011-11-01
|
| Series: | Physical Review X |
| Online Access: | http://doi.org/10.1103/PhysRevX.1.021008 |
| _version_ | 1818727348444332032 |
|---|---|
| author | Hao Qu Yong Wang Chiao-Yu Tseng Giovanni Zocchi |
| author_facet | Hao Qu Yong Wang Chiao-Yu Tseng Giovanni Zocchi |
| author_sort | Hao Qu |
| collection | DOAJ |
| description | We measure the bending energy of double-stranded DNA in the nonlinear (sharply bent) regime. The measurements are obtained from the melting curves of stressed DNA ring molecules. The nonlinear elastic behavior is captured by a single parameter: the critical torque τ_{c} at which the molecule develops a kink. In this regime, the elastic energy is linear in the kink angle. This phenomenology is the same as for the previously reported case of nicked DNA. For the sequences examined, we find τ_{c}=31 pN×nm. This critical torque corresponds to a characteristic energy scale (π/2)τ_{c}=12 kT_{room} relevant for molecular biology processes associated with DNA bending. |
| first_indexed | 2024-12-17T22:12:40Z |
| format | Article |
| id | doaj.art-903eab7e24234a4fabad24a9f9cf63f9 |
| institution | Directory Open Access Journal |
| issn | 2160-3308 |
| language | English |
| last_indexed | 2024-12-17T22:12:40Z |
| publishDate | 2011-11-01 |
| publisher | American Physical Society |
| record_format | Article |
| series | Physical Review X |
| spelling | doaj.art-903eab7e24234a4fabad24a9f9cf63f92022-12-21T21:30:42ZengAmerican Physical SocietyPhysical Review X2160-33082011-11-011202100810.1103/PhysRevX.1.021008Critical Torque for Kink Formation in Double-Stranded DNAHao QuYong WangChiao-Yu TsengGiovanni ZocchiWe measure the bending energy of double-stranded DNA in the nonlinear (sharply bent) regime. The measurements are obtained from the melting curves of stressed DNA ring molecules. The nonlinear elastic behavior is captured by a single parameter: the critical torque τ_{c} at which the molecule develops a kink. In this regime, the elastic energy is linear in the kink angle. This phenomenology is the same as for the previously reported case of nicked DNA. For the sequences examined, we find τ_{c}=31 pN×nm. This critical torque corresponds to a characteristic energy scale (π/2)τ_{c}=12 kT_{room} relevant for molecular biology processes associated with DNA bending.http://doi.org/10.1103/PhysRevX.1.021008 |
| spellingShingle | Hao Qu Yong Wang Chiao-Yu Tseng Giovanni Zocchi Critical Torque for Kink Formation in Double-Stranded DNA Physical Review X |
| title | Critical Torque for Kink Formation in Double-Stranded DNA |
| title_full | Critical Torque for Kink Formation in Double-Stranded DNA |
| title_fullStr | Critical Torque for Kink Formation in Double-Stranded DNA |
| title_full_unstemmed | Critical Torque for Kink Formation in Double-Stranded DNA |
| title_short | Critical Torque for Kink Formation in Double-Stranded DNA |
| title_sort | critical torque for kink formation in double stranded dna |
| url | http://doi.org/10.1103/PhysRevX.1.021008 |
| work_keys_str_mv | AT haoqu criticaltorqueforkinkformationindoublestrandeddna AT yongwang criticaltorqueforkinkformationindoublestrandeddna AT chiaoyutseng criticaltorqueforkinkformationindoublestrandeddna AT giovannizocchi criticaltorqueforkinkformationindoublestrandeddna |