Single-molecule RNA capture-assisted droplet digital loop-mediated isothermal amplification for ultrasensitive and rapid detection of infectious pathogens
Abstract To minimize and control the transmission of infectious diseases, a sensitive, accurate, rapid, and robust assay strategy for application on-site screening is critical. Here, we report single-molecule RNA capture-assisted digital RT-LAMP (SCADL) for point-of-care testing of infectious diseas...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2023-09-01
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| Series: | Microsystems & Nanoengineering |
| Online Access: | https://doi.org/10.1038/s41378-023-00576-2 |
| _version_ | 1797559033999130624 |
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| author | Liying Jiang Xianghao Lan Linjiao Ren Zhiyuan Jin Xuchen Shan Mingzhu Yang Lingqian Chang |
| author_facet | Liying Jiang Xianghao Lan Linjiao Ren Zhiyuan Jin Xuchen Shan Mingzhu Yang Lingqian Chang |
| author_sort | Liying Jiang |
| collection | DOAJ |
| description | Abstract To minimize and control the transmission of infectious diseases, a sensitive, accurate, rapid, and robust assay strategy for application on-site screening is critical. Here, we report single-molecule RNA capture-assisted digital RT-LAMP (SCADL) for point-of-care testing of infectious diseases. Target RNA was captured and enriched by specific capture probes and oligonucleotide probes conjugated to magnetic beads, replacing laborious RNA extraction. Droplet generation, amplification, and the recording of results are all integrated on a microfluidic chip. In assaying commercial standard samples, quantitative results precisely corresponded to the actual concentration of samples. This method provides a limit of detection of 10 copies mL−1 for the N gene within 1 h, greatly reducing the need for skilled personnel and precision instruments. The ultrasensitivity, specificity, portability, rapidity and user-friendliness make SCADL a competitive candidate for the on-site screening of infectious diseases. |
| first_indexed | 2024-03-10T17:40:00Z |
| format | Article |
| id | doaj.art-9042128ff8b740869a38301bfa0df6d2 |
| institution | Directory Open Access Journal |
| issn | 2055-7434 |
| language | English |
| last_indexed | 2024-03-10T17:40:00Z |
| publishDate | 2023-09-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Microsystems & Nanoengineering |
| spelling | doaj.art-9042128ff8b740869a38301bfa0df6d22023-11-20T09:44:42ZengNature Publishing GroupMicrosystems & Nanoengineering2055-74342023-09-019111010.1038/s41378-023-00576-2Single-molecule RNA capture-assisted droplet digital loop-mediated isothermal amplification for ultrasensitive and rapid detection of infectious pathogensLiying Jiang0Xianghao Lan1Linjiao Ren2Zhiyuan Jin3Xuchen Shan4Mingzhu Yang5Lingqian Chang6School of Electrical and Information Engineering, Zhengzhou University of Light IndustrySchool of Electrical and Information Engineering, Zhengzhou University of Light IndustrySchool of Electrical and Information Engineering, Zhengzhou University of Light IndustryBeijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversitySchool of Physics, Beihang UniversityBeijing Research Institute of Mechanical EquipmentBeijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityAbstract To minimize and control the transmission of infectious diseases, a sensitive, accurate, rapid, and robust assay strategy for application on-site screening is critical. Here, we report single-molecule RNA capture-assisted digital RT-LAMP (SCADL) for point-of-care testing of infectious diseases. Target RNA was captured and enriched by specific capture probes and oligonucleotide probes conjugated to magnetic beads, replacing laborious RNA extraction. Droplet generation, amplification, and the recording of results are all integrated on a microfluidic chip. In assaying commercial standard samples, quantitative results precisely corresponded to the actual concentration of samples. This method provides a limit of detection of 10 copies mL−1 for the N gene within 1 h, greatly reducing the need for skilled personnel and precision instruments. The ultrasensitivity, specificity, portability, rapidity and user-friendliness make SCADL a competitive candidate for the on-site screening of infectious diseases.https://doi.org/10.1038/s41378-023-00576-2 |
| spellingShingle | Liying Jiang Xianghao Lan Linjiao Ren Zhiyuan Jin Xuchen Shan Mingzhu Yang Lingqian Chang Single-molecule RNA capture-assisted droplet digital loop-mediated isothermal amplification for ultrasensitive and rapid detection of infectious pathogens Microsystems & Nanoengineering |
| title | Single-molecule RNA capture-assisted droplet digital loop-mediated isothermal amplification for ultrasensitive and rapid detection of infectious pathogens |
| title_full | Single-molecule RNA capture-assisted droplet digital loop-mediated isothermal amplification for ultrasensitive and rapid detection of infectious pathogens |
| title_fullStr | Single-molecule RNA capture-assisted droplet digital loop-mediated isothermal amplification for ultrasensitive and rapid detection of infectious pathogens |
| title_full_unstemmed | Single-molecule RNA capture-assisted droplet digital loop-mediated isothermal amplification for ultrasensitive and rapid detection of infectious pathogens |
| title_short | Single-molecule RNA capture-assisted droplet digital loop-mediated isothermal amplification for ultrasensitive and rapid detection of infectious pathogens |
| title_sort | single molecule rna capture assisted droplet digital loop mediated isothermal amplification for ultrasensitive and rapid detection of infectious pathogens |
| url | https://doi.org/10.1038/s41378-023-00576-2 |
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