Differentiation of Bacillus thuringiensis From Bacilluscereus Group Using a Unique Marker Based on Real-Time PCR
The efficiency of a novel biomarker (the transcriptional regulator, XRE) was tested and evaluated in differentiating Bacillus thuringiensis from Bacillus cereus group species in environmental and spiked samples based on PCR and real-time PCR. Totally 120 strains, representing two bacterial groups, B...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00883/full |
| _version_ | 1811254372317790208 |
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| author | Shuai Wei Ramachandran Chelliah Byung-Jae Park Se-Hun Kim Fereidoun Forghani Min Seok Cho Dong-Suk Park Yong-Guo Jin Deog-Hwan Oh |
| author_facet | Shuai Wei Ramachandran Chelliah Byung-Jae Park Se-Hun Kim Fereidoun Forghani Min Seok Cho Dong-Suk Park Yong-Guo Jin Deog-Hwan Oh |
| author_sort | Shuai Wei |
| collection | DOAJ |
| description | The efficiency of a novel biomarker (the transcriptional regulator, XRE) was tested and evaluated in differentiating Bacillus thuringiensis from Bacillus cereus group species in environmental and spiked samples based on PCR and real-time PCR. Totally 120 strains, representing two bacterial groups, B. cereus group and non-Bacillus sp., were used to evaluate the performance of XRE and crystal protein (cry2, an existing biomarker). Further, three diverse samples (kimbap, lettuce, and spinach) were inoculated with B. thuringiensis and prominent biomarkers XRE and cry2 were used as targets. Direct analysis of the detection results for the pure cultures of B. cereus group wild-types, references and type strains revealed an accuracy rate of 97.5% targeting XRE, and 83.3% targeting cry2. The real-time PCR was constructed with a R2-value of 0.993. For the artificially contaminated samples, a concentration of 103 CFU/g of B. thuringiensis in spiked food samples could be detected using real-time PCR targeting XRE. A good performance was obtained with XRE in discriminating B. thuringiensis from B. cereus groups, as well as detecting B. thuringiensis in spiked food samples with PCR or real-time PCR. Therefore, this real-time PCR targeting XRE can be used as a dependable and promising tool to identify B. thuringiensis in foods. |
| first_indexed | 2024-04-12T17:06:13Z |
| format | Article |
| id | doaj.art-9042c1e9d8944215bc87a8f783e1a750 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T17:06:13Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9042c1e9d8944215bc87a8f783e1a7502022-12-22T03:23:56ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-04-011010.3389/fmicb.2019.00883424164Differentiation of Bacillus thuringiensis From Bacilluscereus Group Using a Unique Marker Based on Real-Time PCRShuai Wei0Ramachandran Chelliah1Byung-Jae Park2Se-Hun Kim3Fereidoun Forghani4Min Seok Cho5Dong-Suk Park6Yong-Guo Jin7Deog-Hwan Oh8Department of Medical Biomaterials Engineering, Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, South KoreaDepartment of Food Science and Biotechnology, Kangwon National University, Chuncheon, South KoreaDepartment of Food Science and Biotechnology, Kangwon National University, Chuncheon, South KoreaDepartment of Food Science and Biotechnology, Kangwon National University, Chuncheon, South KoreaCenter for Food Safety, College of Agricultural and Environmental Sciences, University of Georgia, Griffin, GA, United StatesDepartment of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration, Jeonju, South KoreaDepartment of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration, Jeonju, South KoreaNational Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, ChinaDepartment of Food Science and Biotechnology, Kangwon National University, Chuncheon, South KoreaThe efficiency of a novel biomarker (the transcriptional regulator, XRE) was tested and evaluated in differentiating Bacillus thuringiensis from Bacillus cereus group species in environmental and spiked samples based on PCR and real-time PCR. Totally 120 strains, representing two bacterial groups, B. cereus group and non-Bacillus sp., were used to evaluate the performance of XRE and crystal protein (cry2, an existing biomarker). Further, three diverse samples (kimbap, lettuce, and spinach) were inoculated with B. thuringiensis and prominent biomarkers XRE and cry2 were used as targets. Direct analysis of the detection results for the pure cultures of B. cereus group wild-types, references and type strains revealed an accuracy rate of 97.5% targeting XRE, and 83.3% targeting cry2. The real-time PCR was constructed with a R2-value of 0.993. For the artificially contaminated samples, a concentration of 103 CFU/g of B. thuringiensis in spiked food samples could be detected using real-time PCR targeting XRE. A good performance was obtained with XRE in discriminating B. thuringiensis from B. cereus groups, as well as detecting B. thuringiensis in spiked food samples with PCR or real-time PCR. Therefore, this real-time PCR targeting XRE can be used as a dependable and promising tool to identify B. thuringiensis in foods.https://www.frontiersin.org/article/10.3389/fmicb.2019.00883/fullB. thuringiensistranscriptional regulatorcrystal proteinkimbapspinachlettuce |
| spellingShingle | Shuai Wei Ramachandran Chelliah Byung-Jae Park Se-Hun Kim Fereidoun Forghani Min Seok Cho Dong-Suk Park Yong-Guo Jin Deog-Hwan Oh Differentiation of Bacillus thuringiensis From Bacilluscereus Group Using a Unique Marker Based on Real-Time PCR Frontiers in Microbiology B. thuringiensis transcriptional regulator crystal protein kimbap spinach lettuce |
| title | Differentiation of Bacillus thuringiensis From Bacilluscereus Group Using a Unique Marker Based on Real-Time PCR |
| title_full | Differentiation of Bacillus thuringiensis From Bacilluscereus Group Using a Unique Marker Based on Real-Time PCR |
| title_fullStr | Differentiation of Bacillus thuringiensis From Bacilluscereus Group Using a Unique Marker Based on Real-Time PCR |
| title_full_unstemmed | Differentiation of Bacillus thuringiensis From Bacilluscereus Group Using a Unique Marker Based on Real-Time PCR |
| title_short | Differentiation of Bacillus thuringiensis From Bacilluscereus Group Using a Unique Marker Based on Real-Time PCR |
| title_sort | differentiation of bacillus thuringiensis from bacilluscereus group using a unique marker based on real time pcr |
| topic | B. thuringiensis transcriptional regulator crystal protein kimbap spinach lettuce |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00883/full |
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