A glucan from active dry bakers yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure
The structure of a polysaccharide consisting of D-glucose isolated from the cell-wall of active dry bakers yeast (Saccharomyces cerevisiae) was investigated by using methylation analysis, periodate oxidation, mass spectrometry, NMR spectroscopy, and enzymic hydrolysis, as a new approach in determina...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2003-11-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.shd.org.yu/HtDocs/SHD/Vol68/No11/V68-No11-03.pdf |
| _version_ | 1819138169605455872 |
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| author | DRAGANA ZLATKOVIC DRAGICA JAKOVLJEVIC DJORDJE ZEKOVIC MIROSLAV M. VRVIC |
| author_facet | DRAGANA ZLATKOVIC DRAGICA JAKOVLJEVIC DJORDJE ZEKOVIC MIROSLAV M. VRVIC |
| author_sort | DRAGANA ZLATKOVIC |
| collection | DOAJ |
| description | The structure of a polysaccharide consisting of D-glucose isolated from the cell-wall of active dry bakers yeast (Saccharomyces cerevisiae) was investigated by using methylation analysis, periodate oxidation, mass spectrometry, NMR spectroscopy, and enzymic hydrolysis, as a new approach in determination of structures. The main structural feature of the polysaccharide deduced on the basis of the obtained results is a linear chain of (1->3)-linked b-D-glucopyranoses, a part of which is substituted through the positions O-6. The side units or groups are either a single D-glucopyranose or (1->3)-b-oligoglucosides, linked to the main chaing through (1->6)-glucosidic linkages. The low optical rotation as well as the 13C-NMR and FTIR spectra suggest that the glycosidic linkages are in the b-D-configuration. |
| first_indexed | 2024-12-22T11:02:30Z |
| format | Article |
| id | doaj.art-9043ca6880af42fb89417dc517f3b6c8 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 |
| language | English |
| last_indexed | 2024-12-22T11:02:30Z |
| publishDate | 2003-11-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-9043ca6880af42fb89417dc517f3b6c82022-12-21T18:28:27ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51392003-11-016811805809A glucan from active dry bakers yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structureDRAGANA ZLATKOVICDRAGICA JAKOVLJEVICDJORDJE ZEKOVICMIROSLAV M. VRVICThe structure of a polysaccharide consisting of D-glucose isolated from the cell-wall of active dry bakers yeast (Saccharomyces cerevisiae) was investigated by using methylation analysis, periodate oxidation, mass spectrometry, NMR spectroscopy, and enzymic hydrolysis, as a new approach in determination of structures. The main structural feature of the polysaccharide deduced on the basis of the obtained results is a linear chain of (1->3)-linked b-D-glucopyranoses, a part of which is substituted through the positions O-6. The side units or groups are either a single D-glucopyranose or (1->3)-b-oligoglucosides, linked to the main chaing through (1->6)-glucosidic linkages. The low optical rotation as well as the 13C-NMR and FTIR spectra suggest that the glycosidic linkages are in the b-D-configuration.http://www.shd.org.yu/HtDocs/SHD/Vol68/No11/V68-No11-03.pdfactive dry bakers yeastSaccharomyces cerevisiaepolysaccharidesglucanstructurechemical and enzymatic methods |
| spellingShingle | DRAGANA ZLATKOVIC DRAGICA JAKOVLJEVIC DJORDJE ZEKOVIC MIROSLAV M. VRVIC A glucan from active dry bakers yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure Journal of the Serbian Chemical Society active dry bakers yeast Saccharomyces cerevisiae polysaccharides glucan structure chemical and enzymatic methods |
| title | A glucan from active dry bakers yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure |
| title_full | A glucan from active dry bakers yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure |
| title_fullStr | A glucan from active dry bakers yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure |
| title_full_unstemmed | A glucan from active dry bakers yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure |
| title_short | A glucan from active dry bakers yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure |
| title_sort | glucan from active dry bakers yeast saccharomyces cerevisiae a chemical and enzymatic investigation of the structure |
| topic | active dry bakers yeast Saccharomyces cerevisiae polysaccharides glucan structure chemical and enzymatic methods |
| url | http://www.shd.org.yu/HtDocs/SHD/Vol68/No11/V68-No11-03.pdf |
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