Immunogenicity of ChAdOx1 nCoV-19 Booster Vaccination Following Two CoronaVac Shots in Healthcare Workers
During the early phase of the COVID-19 pandemic, several countries, including Thailand, provided two shots of CoronaVac to healthcare workers. Whereas ChAdOx1 nCoV-19 is the promising vaccine as the booster dose, the data on immunogenicity when administered after CoronaVac have been limited. The pur...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/10/2/217 |
| _version_ | 1827652378635010048 |
|---|---|
| author | Wisit Prasithsirikul Krit Pongpirul Tanawin Nopsopon Phanupong Phutrakool Wannarat Pongpirul Chatpol Samuthpongtorn Pawita Suwanwattana Anan Jongkaewwattana |
| author_facet | Wisit Prasithsirikul Krit Pongpirul Tanawin Nopsopon Phanupong Phutrakool Wannarat Pongpirul Chatpol Samuthpongtorn Pawita Suwanwattana Anan Jongkaewwattana |
| author_sort | Wisit Prasithsirikul |
| collection | DOAJ |
| description | During the early phase of the COVID-19 pandemic, several countries, including Thailand, provided two shots of CoronaVac to healthcare workers. Whereas ChAdOx1 nCoV-19 is the promising vaccine as the booster dose, the data on immunogenicity when administered after CoronaVac have been limited. The purpose of this study was to evaluate the immunogenicity of ChAdOx1 nCoV-19 as the third dose vaccine in healthcare workers who previously received two shots of CoronaVac. The blood samples were obtained before the third vaccination dose, and one month and three months after vaccination. All participants were measured for humoral immunity including anti-spike IgG and neutralizing antibody by ELISA. Twenty participants were stratified by random samples based on baseline IgG status for a cellular immunity function test at three-month post-vaccination, which included T cell and B cell functions by ELISpot. This study showed significant improvement for both humoral and cellular immunity one month after vaccination. Subgroup analysis indicated a significantly higher neutralizing antibody improvement for the population with a negative anti-spike IgG at baseline. Our study suggests that, while immunity level declines at three months post-vaccination, the level was sufficiently high to protect against SARS-CoV-2. |
| first_indexed | 2024-03-09T20:54:39Z |
| format | Article |
| id | doaj.art-9043ee83ffce48ffaaacdda392ed7775 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T20:54:39Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-9043ee83ffce48ffaaacdda392ed77752023-11-23T22:25:25ZengMDPI AGVaccines2076-393X2022-01-0110221710.3390/vaccines10020217Immunogenicity of ChAdOx1 nCoV-19 Booster Vaccination Following Two CoronaVac Shots in Healthcare WorkersWisit Prasithsirikul0Krit Pongpirul1Tanawin Nopsopon2Phanupong Phutrakool3Wannarat Pongpirul4Chatpol Samuthpongtorn5Pawita Suwanwattana6Anan Jongkaewwattana7Bamrasnaradura Infectious Diseases Institute, Nonthaburi 11000, ThailandDepartment of Preventive and Social Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Preventive and Social Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandResearch Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandBamrasnaradura Infectious Diseases Institute, Nonthaburi 11000, ThailandBamrasnaradura Infectious Diseases Institute, Nonthaburi 11000, ThailandBamrasnaradura Infectious Diseases Institute, Nonthaburi 11000, ThailandNational Center of Genetic Engineering and Biotechnology, Khlong Luang, Pathum Thani 12120, ThailandDuring the early phase of the COVID-19 pandemic, several countries, including Thailand, provided two shots of CoronaVac to healthcare workers. Whereas ChAdOx1 nCoV-19 is the promising vaccine as the booster dose, the data on immunogenicity when administered after CoronaVac have been limited. The purpose of this study was to evaluate the immunogenicity of ChAdOx1 nCoV-19 as the third dose vaccine in healthcare workers who previously received two shots of CoronaVac. The blood samples were obtained before the third vaccination dose, and one month and three months after vaccination. All participants were measured for humoral immunity including anti-spike IgG and neutralizing antibody by ELISA. Twenty participants were stratified by random samples based on baseline IgG status for a cellular immunity function test at three-month post-vaccination, which included T cell and B cell functions by ELISpot. This study showed significant improvement for both humoral and cellular immunity one month after vaccination. Subgroup analysis indicated a significantly higher neutralizing antibody improvement for the population with a negative anti-spike IgG at baseline. Our study suggests that, while immunity level declines at three months post-vaccination, the level was sufficiently high to protect against SARS-CoV-2.https://www.mdpi.com/2076-393X/10/2/217COVID-19SARS-CoV-2vaccinesimmunogenicityChAdOx1CoronaVac |
| spellingShingle | Wisit Prasithsirikul Krit Pongpirul Tanawin Nopsopon Phanupong Phutrakool Wannarat Pongpirul Chatpol Samuthpongtorn Pawita Suwanwattana Anan Jongkaewwattana Immunogenicity of ChAdOx1 nCoV-19 Booster Vaccination Following Two CoronaVac Shots in Healthcare Workers Vaccines COVID-19 SARS-CoV-2 vaccines immunogenicity ChAdOx1 CoronaVac |
| title | Immunogenicity of ChAdOx1 nCoV-19 Booster Vaccination Following Two CoronaVac Shots in Healthcare Workers |
| title_full | Immunogenicity of ChAdOx1 nCoV-19 Booster Vaccination Following Two CoronaVac Shots in Healthcare Workers |
| title_fullStr | Immunogenicity of ChAdOx1 nCoV-19 Booster Vaccination Following Two CoronaVac Shots in Healthcare Workers |
| title_full_unstemmed | Immunogenicity of ChAdOx1 nCoV-19 Booster Vaccination Following Two CoronaVac Shots in Healthcare Workers |
| title_short | Immunogenicity of ChAdOx1 nCoV-19 Booster Vaccination Following Two CoronaVac Shots in Healthcare Workers |
| title_sort | immunogenicity of chadox1 ncov 19 booster vaccination following two coronavac shots in healthcare workers |
| topic | COVID-19 SARS-CoV-2 vaccines immunogenicity ChAdOx1 CoronaVac |
| url | https://www.mdpi.com/2076-393X/10/2/217 |
| work_keys_str_mv | AT wisitprasithsirikul immunogenicityofchadox1ncov19boostervaccinationfollowingtwocoronavacshotsinhealthcareworkers AT kritpongpirul immunogenicityofchadox1ncov19boostervaccinationfollowingtwocoronavacshotsinhealthcareworkers AT tanawinnopsopon immunogenicityofchadox1ncov19boostervaccinationfollowingtwocoronavacshotsinhealthcareworkers AT phanupongphutrakool immunogenicityofchadox1ncov19boostervaccinationfollowingtwocoronavacshotsinhealthcareworkers AT wannaratpongpirul immunogenicityofchadox1ncov19boostervaccinationfollowingtwocoronavacshotsinhealthcareworkers AT chatpolsamuthpongtorn immunogenicityofchadox1ncov19boostervaccinationfollowingtwocoronavacshotsinhealthcareworkers AT pawitasuwanwattana immunogenicityofchadox1ncov19boostervaccinationfollowingtwocoronavacshotsinhealthcareworkers AT ananjongkaewwattana immunogenicityofchadox1ncov19boostervaccinationfollowingtwocoronavacshotsinhealthcareworkers |