Isolation and identification of equine herpesvirus -1 (EHV-1) in Egypt during 2021-2022
Equine herpes virus-1 (EHV-1) is a major cause of economic losses in horse industry all over the world especially in the Arabian countries. The aim of the study is the isolation and identification of the local equine herpesvirus-1 at northern Egypt Governorates (Cairo, Dakahlyia, Qalyubia) during 2...
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| Format: | Article |
| Language: | English |
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Assiut University
2024-03-01
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| Series: | Journal of Advanced Veterinary Research |
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| Online Access: | https://www.advetresearch.com/index.php/AVR/article/view/1757 |
| _version_ | 1797226430210244608 |
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| author | Ahmed A.H. Ali Fatma Abdallah Omayma A. Shemies Gamilat Kotb Maged R. Nafea |
| author_facet | Ahmed A.H. Ali Fatma Abdallah Omayma A. Shemies Gamilat Kotb Maged R. Nafea |
| author_sort | Ahmed A.H. Ali |
| collection | DOAJ |
| description |
Equine herpes virus-1 (EHV-1) is a major cause of economic losses in horse industry all over the world especially in the Arabian countries. The aim of the study is the isolation and identification of the local equine herpesvirus-1 at northern Egypt Governorates (Cairo, Dakahlyia, Qalyubia) during 2021- 2022. A total of 50 tissue organs were collected from aborted fetuses of affected mares recently after abortion. The collected samples were screened using consensus PCR for detection of equine herpesviruses (EHVs) using specific primers targeting DNA polymerase gene which revealed that 26 out of 50 samples were positive with percentage of 52%.The molecular positive samples were subjected to conventional PCR for further molecular identification and typing of EHVs using specific primers targeting glycoprotein (gB) gene which confirmed that all amplified products were for EHV-1. Attempts for isolation of EHV-1 were performed through propagation of molecular identified samples on Madin-Darby bovine kidney (MDBK) cell line which revealed that 19 samples developed characteristic cytopathic effect in form of cell rounding and rapidly enlarging grapes like cell aggregation, and then detached of the cell, while 13 samples induced pock lesions on CAMs of SPF-ECE after inoculation for three successive passages. Serological identification of EHV-1 isolates was confirmed by immunofluorescent antibody test (IFAT) which showed that 19 samples induced intracytoplasmic greenish yellow fluorescence. Selected EHV-1 isolate was subjected for transmission electron microscopy (TEM) for demonstration of virus morphology. The obtained results confirmed that EHV-1 is the major cause of abortion among Arabian mares which disrupts breeding and showcasing events. Future considerations about management strategies together with regular and effective vaccine trials are recommended for control of EHV-1.
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| first_indexed | 2024-04-24T14:24:47Z |
| format | Article |
| id | doaj.art-9044f410ba9b4e6bb8ca4cf36a82d2c6 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-04-24T14:24:47Z |
| publishDate | 2024-03-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-9044f410ba9b4e6bb8ca4cf36a82d2c62024-04-03T05:12:09ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772024-03-01143Isolation and identification of equine herpesvirus -1 (EHV-1) in Egypt during 2021-2022 Ahmed A.H. Ali0Fatma Abdallah1Omayma A. Shemies2Gamilat Kotb3Maged R. Nafea4Department of Virology, Faculty of Veterinary Medicine, Zagazig University, Egypt.Department of Virology, Faculty of Veterinary Medicine, Zagazig University, Egypt.Agricultural Research Center (ARC), Animal Health Research Institute (AHRI), Dokki, Egypt.Department of Virology, Faculty of Veterinary Medicine, Zagazig University, Egypt. Agricultural Research Center (ARC), Animal Health Research Institute (AHRI), Dokki, Egypt. Equine herpes virus-1 (EHV-1) is a major cause of economic losses in horse industry all over the world especially in the Arabian countries. The aim of the study is the isolation and identification of the local equine herpesvirus-1 at northern Egypt Governorates (Cairo, Dakahlyia, Qalyubia) during 2021- 2022. A total of 50 tissue organs were collected from aborted fetuses of affected mares recently after abortion. The collected samples were screened using consensus PCR for detection of equine herpesviruses (EHVs) using specific primers targeting DNA polymerase gene which revealed that 26 out of 50 samples were positive with percentage of 52%.The molecular positive samples were subjected to conventional PCR for further molecular identification and typing of EHVs using specific primers targeting glycoprotein (gB) gene which confirmed that all amplified products were for EHV-1. Attempts for isolation of EHV-1 were performed through propagation of molecular identified samples on Madin-Darby bovine kidney (MDBK) cell line which revealed that 19 samples developed characteristic cytopathic effect in form of cell rounding and rapidly enlarging grapes like cell aggregation, and then detached of the cell, while 13 samples induced pock lesions on CAMs of SPF-ECE after inoculation for three successive passages. Serological identification of EHV-1 isolates was confirmed by immunofluorescent antibody test (IFAT) which showed that 19 samples induced intracytoplasmic greenish yellow fluorescence. Selected EHV-1 isolate was subjected for transmission electron microscopy (TEM) for demonstration of virus morphology. The obtained results confirmed that EHV-1 is the major cause of abortion among Arabian mares which disrupts breeding and showcasing events. Future considerations about management strategies together with regular and effective vaccine trials are recommended for control of EHV-1. https://www.advetresearch.com/index.php/AVR/article/view/1757EHV-1 Abortion Arabian horses Isolation EgyptPCR |
| spellingShingle | Ahmed A.H. Ali Fatma Abdallah Omayma A. Shemies Gamilat Kotb Maged R. Nafea Isolation and identification of equine herpesvirus -1 (EHV-1) in Egypt during 2021-2022 Journal of Advanced Veterinary Research EHV-1 Abortion Arabian horses Isolation Egypt PCR |
| title | Isolation and identification of equine herpesvirus -1 (EHV-1) in Egypt during 2021-2022 |
| title_full | Isolation and identification of equine herpesvirus -1 (EHV-1) in Egypt during 2021-2022 |
| title_fullStr | Isolation and identification of equine herpesvirus -1 (EHV-1) in Egypt during 2021-2022 |
| title_full_unstemmed | Isolation and identification of equine herpesvirus -1 (EHV-1) in Egypt during 2021-2022 |
| title_short | Isolation and identification of equine herpesvirus -1 (EHV-1) in Egypt during 2021-2022 |
| title_sort | isolation and identification of equine herpesvirus 1 ehv 1 in egypt during 2021 2022 |
| topic | EHV-1 Abortion Arabian horses Isolation Egypt PCR |
| url | https://www.advetresearch.com/index.php/AVR/article/view/1757 |
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