Bare Iron Oxide Nanoparticles as Drug Delivery Carrier for the Short Cationic Peptide Lasioglossin
New drug delivery systems are a potential solution for administering drugs to reduce common side effects of traditional methods, such as in cancer therapy. Iron oxide nanoparticles (IONs) can increase the drugs’ biological activity through high binding efficiency and magnetically targeted drug deliv...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/14/5/405 |
| _version_ | 1797536463291678720 |
|---|---|
| author | Chiara Turrina Sonja Berensmeier Sebastian P. Schwaminger |
| author_facet | Chiara Turrina Sonja Berensmeier Sebastian P. Schwaminger |
| author_sort | Chiara Turrina |
| collection | DOAJ |
| description | New drug delivery systems are a potential solution for administering drugs to reduce common side effects of traditional methods, such as in cancer therapy. Iron oxide nanoparticles (IONs) can increase the drugs’ biological activity through high binding efficiency and magnetically targeted drug delivery. Understanding the adsorption and release process of a drug to the carrier material plays a significant role in research to generate an applicable and controlled drug delivery system. This contribution focuses on the binding patterns of the peptide lasioglossin III from bee venom on bare IONs. Lasioglossin has a high antimicrobial behavior and due to its cationic properties, it has high binding potential. Considering the influence of pH, the buffer type, the particle concentration, and time, the highest drug loading of 22.7% is achieved in phosphate-buffered saline. Analysis of the desorption conditions revealed temperature and salt concentration sensitivity. The nanoparticles and peptide-ION complexes are analyzed with dynamic light scattering, zeta potential, and infrared spectroscopy. Additionally, cytotoxicity experiments performed on <i>Escherichia coli</i> show higher antimicrobial activity of bound lasioglossin than of the free peptide. Therefore, bare IONs are an interesting platform material for the development of drug-delivery carriers for cationic peptides. |
| first_indexed | 2024-03-10T12:01:02Z |
| format | Article |
| id | doaj.art-9045da2fec814c54a1a0a664b2ee91c8 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T12:01:02Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-9045da2fec814c54a1a0a664b2ee91c82023-11-21T16:58:45ZengMDPI AGPharmaceuticals1424-82472021-04-0114540510.3390/ph14050405Bare Iron Oxide Nanoparticles as Drug Delivery Carrier for the Short Cationic Peptide LasioglossinChiara Turrina0Sonja Berensmeier1Sebastian P. Schwaminger2Bioseparation Engineering Group, Department of Mechanical Engineering, Technical University of Munich, 80333 München, GermanyBioseparation Engineering Group, Department of Mechanical Engineering, Technical University of Munich, 80333 München, GermanyBioseparation Engineering Group, Department of Mechanical Engineering, Technical University of Munich, 80333 München, GermanyNew drug delivery systems are a potential solution for administering drugs to reduce common side effects of traditional methods, such as in cancer therapy. Iron oxide nanoparticles (IONs) can increase the drugs’ biological activity through high binding efficiency and magnetically targeted drug delivery. Understanding the adsorption and release process of a drug to the carrier material plays a significant role in research to generate an applicable and controlled drug delivery system. This contribution focuses on the binding patterns of the peptide lasioglossin III from bee venom on bare IONs. Lasioglossin has a high antimicrobial behavior and due to its cationic properties, it has high binding potential. Considering the influence of pH, the buffer type, the particle concentration, and time, the highest drug loading of 22.7% is achieved in phosphate-buffered saline. Analysis of the desorption conditions revealed temperature and salt concentration sensitivity. The nanoparticles and peptide-ION complexes are analyzed with dynamic light scattering, zeta potential, and infrared spectroscopy. Additionally, cytotoxicity experiments performed on <i>Escherichia coli</i> show higher antimicrobial activity of bound lasioglossin than of the free peptide. Therefore, bare IONs are an interesting platform material for the development of drug-delivery carriers for cationic peptides.https://www.mdpi.com/1424-8247/14/5/405iron oxide nanoparticlesmagnetically controlled drug deliverycationic peptidelasioglossinagglomeration behavior in human serumantimicrobial behavior |
| spellingShingle | Chiara Turrina Sonja Berensmeier Sebastian P. Schwaminger Bare Iron Oxide Nanoparticles as Drug Delivery Carrier for the Short Cationic Peptide Lasioglossin Pharmaceuticals iron oxide nanoparticles magnetically controlled drug delivery cationic peptide lasioglossin agglomeration behavior in human serum antimicrobial behavior |
| title | Bare Iron Oxide Nanoparticles as Drug Delivery Carrier for the Short Cationic Peptide Lasioglossin |
| title_full | Bare Iron Oxide Nanoparticles as Drug Delivery Carrier for the Short Cationic Peptide Lasioglossin |
| title_fullStr | Bare Iron Oxide Nanoparticles as Drug Delivery Carrier for the Short Cationic Peptide Lasioglossin |
| title_full_unstemmed | Bare Iron Oxide Nanoparticles as Drug Delivery Carrier for the Short Cationic Peptide Lasioglossin |
| title_short | Bare Iron Oxide Nanoparticles as Drug Delivery Carrier for the Short Cationic Peptide Lasioglossin |
| title_sort | bare iron oxide nanoparticles as drug delivery carrier for the short cationic peptide lasioglossin |
| topic | iron oxide nanoparticles magnetically controlled drug delivery cationic peptide lasioglossin agglomeration behavior in human serum antimicrobial behavior |
| url | https://www.mdpi.com/1424-8247/14/5/405 |
| work_keys_str_mv | AT chiaraturrina bareironoxidenanoparticlesasdrugdeliverycarrierfortheshortcationicpeptidelasioglossin AT sonjaberensmeier bareironoxidenanoparticlesasdrugdeliverycarrierfortheshortcationicpeptidelasioglossin AT sebastianpschwaminger bareironoxidenanoparticlesasdrugdeliverycarrierfortheshortcationicpeptidelasioglossin |