Differential Roles of Dendritic Cells in Expanding CD4 T Cells in Sepsis
Sepsis is a systemically dysregulated inflammatory syndrome, in which dendritic cells (DCs) play a critical role in coordinating aberrant immunity. The aim of this study is to shed light on the differential roles played by systemic versus mucosal DCs in regulating immune responses in sepsis. We iden...
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| Format: | Article |
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MDPI AG
2019-07-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/7/3/52 |
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| author | Samuel Darkwah Nodoka Nago Michael G. Appiah Phyoe Kyawe Myint Eiji Kawamoto Motomu Shimaoka Eun Jeong Park |
| author_facet | Samuel Darkwah Nodoka Nago Michael G. Appiah Phyoe Kyawe Myint Eiji Kawamoto Motomu Shimaoka Eun Jeong Park |
| author_sort | Samuel Darkwah |
| collection | DOAJ |
| description | Sepsis is a systemically dysregulated inflammatory syndrome, in which dendritic cells (DCs) play a critical role in coordinating aberrant immunity. The aim of this study is to shed light on the differential roles played by systemic versus mucosal DCs in regulating immune responses in sepsis. We identified a differential impact of the systemic and mucosal DCs on proliferating allogenic CD4 T cells in a mouse model of sepsis. Despite the fact that the frequency of CD4 T cells was reduced in septic mice, septic mesenteric lymph node (MLN) DCs proved superior to septic spleen (SP) DCs in expanding allogeneic CD4 T cells. Moreover, septic MLN DCs markedly augmented the surface expression of MHC class II and CD40, as well as the messaging of interleukin-1β (IL-1β). Interestingly, IL-1β-treated CD4 T cells expanded in a dose-dependent manner, suggesting that this cytokine acts as a key mediator of MLN DCs in promoting septic inflammation. Thus, mucosal and systemic DCs were found to be functionally different in the way CD4 T cells respond during sepsis. Our study provides a molecular basis for DC activity, which can be differential in nature depending on location, whereby it induces septic inflammation or immune-paralysis. |
| first_indexed | 2024-12-24T23:05:04Z |
| format | Article |
| id | doaj.art-90471cec829a48369ac9dd6818e69c75 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-12-24T23:05:04Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-90471cec829a48369ac9dd6818e69c752022-12-21T16:35:02ZengMDPI AGBiomedicines2227-90592019-07-01735210.3390/biomedicines7030052biomedicines7030052Differential Roles of Dendritic Cells in Expanding CD4 T Cells in SepsisSamuel Darkwah0Nodoka Nago1Michael G. Appiah2Phyoe Kyawe Myint3Eiji Kawamoto4Motomu Shimaoka5Eun Jeong Park6Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, JapanDepartment of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, JapanSepsis is a systemically dysregulated inflammatory syndrome, in which dendritic cells (DCs) play a critical role in coordinating aberrant immunity. The aim of this study is to shed light on the differential roles played by systemic versus mucosal DCs in regulating immune responses in sepsis. We identified a differential impact of the systemic and mucosal DCs on proliferating allogenic CD4 T cells in a mouse model of sepsis. Despite the fact that the frequency of CD4 T cells was reduced in septic mice, septic mesenteric lymph node (MLN) DCs proved superior to septic spleen (SP) DCs in expanding allogeneic CD4 T cells. Moreover, septic MLN DCs markedly augmented the surface expression of MHC class II and CD40, as well as the messaging of interleukin-1β (IL-1β). Interestingly, IL-1β-treated CD4 T cells expanded in a dose-dependent manner, suggesting that this cytokine acts as a key mediator of MLN DCs in promoting septic inflammation. Thus, mucosal and systemic DCs were found to be functionally different in the way CD4 T cells respond during sepsis. Our study provides a molecular basis for DC activity, which can be differential in nature depending on location, whereby it induces septic inflammation or immune-paralysis.https://www.mdpi.com/2227-9059/7/3/52sepsisspleenmesenteric lymph nodesdendritic cellsCD4 T cellsIL-1β |
| spellingShingle | Samuel Darkwah Nodoka Nago Michael G. Appiah Phyoe Kyawe Myint Eiji Kawamoto Motomu Shimaoka Eun Jeong Park Differential Roles of Dendritic Cells in Expanding CD4 T Cells in Sepsis Biomedicines sepsis spleen mesenteric lymph nodes dendritic cells CD4 T cells IL-1β |
| title | Differential Roles of Dendritic Cells in Expanding CD4 T Cells in Sepsis |
| title_full | Differential Roles of Dendritic Cells in Expanding CD4 T Cells in Sepsis |
| title_fullStr | Differential Roles of Dendritic Cells in Expanding CD4 T Cells in Sepsis |
| title_full_unstemmed | Differential Roles of Dendritic Cells in Expanding CD4 T Cells in Sepsis |
| title_short | Differential Roles of Dendritic Cells in Expanding CD4 T Cells in Sepsis |
| title_sort | differential roles of dendritic cells in expanding cd4 t cells in sepsis |
| topic | sepsis spleen mesenteric lymph nodes dendritic cells CD4 T cells IL-1β |
| url | https://www.mdpi.com/2227-9059/7/3/52 |
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