Pre- and Postsynaptic Role of Dopamine D2 Receptor DD2R in Drosophila Olfactory Associative Learning
Dopaminergic neurons in Drosophila play critical roles in diverse brain functions such as motor control, arousal, learning, and memory. Using genetic and behavioral approaches, it has been firmly established that proper dopamine signaling is required for olfactory classical conditioning (e.g., avers...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-11-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-7737/3/4/831 |
| _version_ | 1827884908566020096 |
|---|---|
| author | Cheng Qi Daewoo Lee |
| author_facet | Cheng Qi Daewoo Lee |
| author_sort | Cheng Qi |
| collection | DOAJ |
| description | Dopaminergic neurons in Drosophila play critical roles in diverse brain functions such as motor control, arousal, learning, and memory. Using genetic and behavioral approaches, it has been firmly established that proper dopamine signaling is required for olfactory classical conditioning (e.g., aversive and appetitive learning). Dopamine mediates its functions through interaction with its receptors. There are two different types of dopamine receptors in Drosophila: D1-like (dDA1, DAMB) and D2-like receptors (DD2R). Currently, no study has attempted to characterize the role of DD2R in Drosophila learning and memory. Using a DD2R-RNAi transgenic line, we have examined the role of DD2R, expressed in dopamine neurons (i.e., the presynaptic DD2R autoreceptor), in larval olfactory learning. The function of postsynaptic DD2R expressed in mushroom body (MB) was also studied as MB is the center for Drosophila learning, with a function analogous to that of the mammalian hippocampus. Our results showed that suppression of presynaptic DD2R autoreceptors impairs both appetitive and aversive learning. Similarly, postsynaptic DD2R in MB neurons appears to be involved in both appetitive and aversive learning. The data confirm, for the first time, that DD2R plays an important role in Drosophila olfactory learning. |
| first_indexed | 2024-03-12T19:36:38Z |
| format | Article |
| id | doaj.art-9048347df09b4e3ea0e265d524b37b09 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-12T19:36:38Z |
| publishDate | 2014-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-9048347df09b4e3ea0e265d524b37b092023-08-02T04:07:45ZengMDPI AGBiology2079-77372014-11-013483184510.3390/biology3040831biology3040831Pre- and Postsynaptic Role of Dopamine D2 Receptor DD2R in Drosophila Olfactory Associative LearningCheng Qi0Daewoo Lee1Neuroscience Program, Department of Biological Sciences, Ohio University, Athens, OH 45701, USANeuroscience Program, Department of Biological Sciences, Ohio University, Athens, OH 45701, USADopaminergic neurons in Drosophila play critical roles in diverse brain functions such as motor control, arousal, learning, and memory. Using genetic and behavioral approaches, it has been firmly established that proper dopamine signaling is required for olfactory classical conditioning (e.g., aversive and appetitive learning). Dopamine mediates its functions through interaction with its receptors. There are two different types of dopamine receptors in Drosophila: D1-like (dDA1, DAMB) and D2-like receptors (DD2R). Currently, no study has attempted to characterize the role of DD2R in Drosophila learning and memory. Using a DD2R-RNAi transgenic line, we have examined the role of DD2R, expressed in dopamine neurons (i.e., the presynaptic DD2R autoreceptor), in larval olfactory learning. The function of postsynaptic DD2R expressed in mushroom body (MB) was also studied as MB is the center for Drosophila learning, with a function analogous to that of the mammalian hippocampus. Our results showed that suppression of presynaptic DD2R autoreceptors impairs both appetitive and aversive learning. Similarly, postsynaptic DD2R in MB neurons appears to be involved in both appetitive and aversive learning. The data confirm, for the first time, that DD2R plays an important role in Drosophila olfactory learning.http://www.mdpi.com/2079-7737/3/4/831Drosophila larvaeaversive learningappetitive learningdopaminergic neuronsmushroom body |
| spellingShingle | Cheng Qi Daewoo Lee Pre- and Postsynaptic Role of Dopamine D2 Receptor DD2R in Drosophila Olfactory Associative Learning Biology Drosophila larvae aversive learning appetitive learning dopaminergic neurons mushroom body |
| title | Pre- and Postsynaptic Role of Dopamine D2 Receptor DD2R in Drosophila Olfactory Associative Learning |
| title_full | Pre- and Postsynaptic Role of Dopamine D2 Receptor DD2R in Drosophila Olfactory Associative Learning |
| title_fullStr | Pre- and Postsynaptic Role of Dopamine D2 Receptor DD2R in Drosophila Olfactory Associative Learning |
| title_full_unstemmed | Pre- and Postsynaptic Role of Dopamine D2 Receptor DD2R in Drosophila Olfactory Associative Learning |
| title_short | Pre- and Postsynaptic Role of Dopamine D2 Receptor DD2R in Drosophila Olfactory Associative Learning |
| title_sort | pre and postsynaptic role of dopamine d2 receptor dd2r in drosophila olfactory associative learning |
| topic | Drosophila larvae aversive learning appetitive learning dopaminergic neurons mushroom body |
| url | http://www.mdpi.com/2079-7737/3/4/831 |
| work_keys_str_mv | AT chengqi preandpostsynapticroleofdopamined2receptordd2rindrosophilaolfactoryassociativelearning AT daewoolee preandpostsynapticroleofdopamined2receptordd2rindrosophilaolfactoryassociativelearning |