Light sheet fluorescence microscopy of cleared human eyes
Abstract We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling. Demonstrated here in four eyes, post-processing of LSFM stacks enables three-dimensional (3D) navigation and customized display,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-023-05401-0 |
| _version_ | 1797557154796797952 |
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| author | Marie Darche Ysé Borella Anna Verschueren Ivana Gantar Stéphane Pagès Laura Batti Michel Paques |
| author_facet | Marie Darche Ysé Borella Anna Verschueren Ivana Gantar Stéphane Pagès Laura Batti Michel Paques |
| author_sort | Marie Darche |
| collection | DOAJ |
| description | Abstract We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling. Demonstrated here in four eyes, post-processing of LSFM stacks enables three-dimensional (3D) navigation and customized display, including en face viewing of the fundus similarly to clinical imaging, with resolution of retinal capillaries. This method overcomes several limitations of traditional histology of the eyes. Tracing of spatially complex structures such as anterior ciliary vessels and Schlemm’s canal was achieved. We conclude that LSFM of immunolabeled human eyes after iDISCO + -based clearing is a powerful tool for 3D histology of large human ocular samples, including entire eyes, which will be useful in both anatomopathology and in research. |
| first_indexed | 2024-03-10T17:13:19Z |
| format | Article |
| id | doaj.art-904a0c3427094c27b8a851a715165422 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-03-10T17:13:19Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-904a0c3427094c27b8a851a7151654222023-11-20T10:35:37ZengNature PortfolioCommunications Biology2399-36422023-10-01611710.1038/s42003-023-05401-0Light sheet fluorescence microscopy of cleared human eyesMarie Darche0Ysé Borella1Anna Verschueren2Ivana Gantar3Stéphane Pagès4Laura Batti5Michel Paques6Paris Eye Imaging Group, 15-20 Hôpital National de la Vision, INSERM-DHOS Clinical Investigation CenterParis Eye Imaging Group, 15-20 Hôpital National de la Vision, INSERM-DHOS Clinical Investigation CenterParis Eye Imaging Group, 15-20 Hôpital National de la Vision, INSERM-DHOS Clinical Investigation CenterWyss Center for Bio- and NeuroengineeringWyss Center for Bio- and NeuroengineeringWyss Center for Bio- and NeuroengineeringParis Eye Imaging Group, 15-20 Hôpital National de la Vision, INSERM-DHOS Clinical Investigation CenterAbstract We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling. Demonstrated here in four eyes, post-processing of LSFM stacks enables three-dimensional (3D) navigation and customized display, including en face viewing of the fundus similarly to clinical imaging, with resolution of retinal capillaries. This method overcomes several limitations of traditional histology of the eyes. Tracing of spatially complex structures such as anterior ciliary vessels and Schlemm’s canal was achieved. We conclude that LSFM of immunolabeled human eyes after iDISCO + -based clearing is a powerful tool for 3D histology of large human ocular samples, including entire eyes, which will be useful in both anatomopathology and in research.https://doi.org/10.1038/s42003-023-05401-0 |
| spellingShingle | Marie Darche Ysé Borella Anna Verschueren Ivana Gantar Stéphane Pagès Laura Batti Michel Paques Light sheet fluorescence microscopy of cleared human eyes Communications Biology |
| title | Light sheet fluorescence microscopy of cleared human eyes |
| title_full | Light sheet fluorescence microscopy of cleared human eyes |
| title_fullStr | Light sheet fluorescence microscopy of cleared human eyes |
| title_full_unstemmed | Light sheet fluorescence microscopy of cleared human eyes |
| title_short | Light sheet fluorescence microscopy of cleared human eyes |
| title_sort | light sheet fluorescence microscopy of cleared human eyes |
| url | https://doi.org/10.1038/s42003-023-05401-0 |
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