Light sheet fluorescence microscopy of cleared human eyes

Light sheet fluorescence microscopy of cleared human eyes

Abstract We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling. Demonstrated here in four eyes, post-processing of LSFM stacks enables three-dimensional (3D) navigation and customized display,...

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Bibliographic Details
Main Authors:	Marie Darche, Ysé Borella, Anna Verschueren, Ivana Gantar, Stéphane Pagès, Laura Batti, Michel Paques
Format:	Article
Language:	English
Published:	Nature Portfolio 2023-10-01
Series:	Communications Biology
Online Access:	https://doi.org/10.1038/s42003-023-05401-0

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