Classification and Functional Characterization of Vasa Vasorum-Associated Perivascular Progenitor Cells in Human Aorta
In the microcirculation, pericytes are believed to function as mesenchymal stromal cells (MSCs). We hypothesized that the vasa vasorum harbor progenitor cells within the adventitia of human aorta. Pericytes, endothelial progenitor cells, and other cell subpopulations were detected among freshly isol...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-07-01
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| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671117301820 |
| _version_ | 1818854325951135744 |
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| author | Marie Billaud Vera S. Donnenberg Bradley W. Ellis E. Michael Meyer Albert D. Donnenberg Jennifer C. Hill Tara D. Richards Thomas G. Gleason Julie A. Phillippi |
| author_facet | Marie Billaud Vera S. Donnenberg Bradley W. Ellis E. Michael Meyer Albert D. Donnenberg Jennifer C. Hill Tara D. Richards Thomas G. Gleason Julie A. Phillippi |
| author_sort | Marie Billaud |
| collection | DOAJ |
| description | In the microcirculation, pericytes are believed to function as mesenchymal stromal cells (MSCs). We hypothesized that the vasa vasorum harbor progenitor cells within the adventitia of human aorta. Pericytes, endothelial progenitor cells, and other cell subpopulations were detected among freshly isolated adventitial cells using flow cytometry. Purified cultured pericytes were enriched for the MSC markers CD105 and CD73 and depleted of the endothelial markers von Willebrand factor and CD31. Cultured pericytes were capable of smooth muscle lineage progression including inducible expression of smooth muscle myosin heavy chain, calponin, and α-smooth muscle actin, and adopted a spindle shape. Pericytes formed spheroids when cultured on Matrigel substrates and peripherally localized with branching endothelial cells in vitro. Our results indicate that the vasa vasorum form a progenitor cell niche distinct from other previously described progenitor populations in human adventitia. These findings could have important implications for understanding the complex pathophysiology of human aortic disease. |
| first_indexed | 2024-12-19T07:50:55Z |
| format | Article |
| id | doaj.art-904aea15677a4cc89b1885fb66b8a79e |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-19T07:50:55Z |
| publishDate | 2017-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-904aea15677a4cc89b1885fb66b8a79e2022-12-21T20:30:11ZengElsevierStem Cell Reports2213-67112017-07-019129230310.1016/j.stemcr.2017.04.028Classification and Functional Characterization of Vasa Vasorum-Associated Perivascular Progenitor Cells in Human AortaMarie Billaud0Vera S. Donnenberg1Bradley W. Ellis2E. Michael Meyer3Albert D. Donnenberg4Jennifer C. Hill5Tara D. Richards6Thomas G. Gleason7Julie A. Phillippi8Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USADepartment of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USADepartment of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USAUniversity of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, PA 15213, USAMcGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USADepartment of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USADepartment of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USADepartment of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USADepartment of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USAIn the microcirculation, pericytes are believed to function as mesenchymal stromal cells (MSCs). We hypothesized that the vasa vasorum harbor progenitor cells within the adventitia of human aorta. Pericytes, endothelial progenitor cells, and other cell subpopulations were detected among freshly isolated adventitial cells using flow cytometry. Purified cultured pericytes were enriched for the MSC markers CD105 and CD73 and depleted of the endothelial markers von Willebrand factor and CD31. Cultured pericytes were capable of smooth muscle lineage progression including inducible expression of smooth muscle myosin heavy chain, calponin, and α-smooth muscle actin, and adopted a spindle shape. Pericytes formed spheroids when cultured on Matrigel substrates and peripherally localized with branching endothelial cells in vitro. Our results indicate that the vasa vasorum form a progenitor cell niche distinct from other previously described progenitor populations in human adventitia. These findings could have important implications for understanding the complex pathophysiology of human aortic disease.http://www.sciencedirect.com/science/article/pii/S2213671117301820perivascular progenitor cellspericyteadventitiaaortaendothelial progenitor cellsflow cytometry |
| spellingShingle | Marie Billaud Vera S. Donnenberg Bradley W. Ellis E. Michael Meyer Albert D. Donnenberg Jennifer C. Hill Tara D. Richards Thomas G. Gleason Julie A. Phillippi Classification and Functional Characterization of Vasa Vasorum-Associated Perivascular Progenitor Cells in Human Aorta Stem Cell Reports perivascular progenitor cells pericyte adventitia aorta endothelial progenitor cells flow cytometry |
| title | Classification and Functional Characterization of Vasa Vasorum-Associated Perivascular Progenitor Cells in Human Aorta |
| title_full | Classification and Functional Characterization of Vasa Vasorum-Associated Perivascular Progenitor Cells in Human Aorta |
| title_fullStr | Classification and Functional Characterization of Vasa Vasorum-Associated Perivascular Progenitor Cells in Human Aorta |
| title_full_unstemmed | Classification and Functional Characterization of Vasa Vasorum-Associated Perivascular Progenitor Cells in Human Aorta |
| title_short | Classification and Functional Characterization of Vasa Vasorum-Associated Perivascular Progenitor Cells in Human Aorta |
| title_sort | classification and functional characterization of vasa vasorum associated perivascular progenitor cells in human aorta |
| topic | perivascular progenitor cells pericyte adventitia aorta endothelial progenitor cells flow cytometry |
| url | http://www.sciencedirect.com/science/article/pii/S2213671117301820 |
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