An Aluminum-Based Microfluidic Chip for Polymerase Chain Reaction Diagnosis
Real-time polymerase chain reaction (real-time PCR) tests were successfully conducted in an aluminum-based microfluidic chip developed in this work. The reaction chamber was coated with silicone-modified epoxy resin to isolate the reaction system from metal surfaces, preventing the metal ions from i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/3/1085 |
| _version_ | 1797623775103025152 |
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| author | Siyu Yang Ziyi Zhang Qingyue Xian Qi Song Yiteng Liu Yibo Gao Weijia Wen |
| author_facet | Siyu Yang Ziyi Zhang Qingyue Xian Qi Song Yiteng Liu Yibo Gao Weijia Wen |
| author_sort | Siyu Yang |
| collection | DOAJ |
| description | Real-time polymerase chain reaction (real-time PCR) tests were successfully conducted in an aluminum-based microfluidic chip developed in this work. The reaction chamber was coated with silicone-modified epoxy resin to isolate the reaction system from metal surfaces, preventing the metal ions from interfering with the reaction process. The patterned aluminum substrate was bonded with a hydroxylated glass mask using silicone sealant at room temperature. The effect of thermal expansion was counteracted by the elasticity of cured silicone. With the heating process closely monitored, real-time PCR testing in reaction chambers proceeded smoothly, and the results show similar quantification cycle values to those of traditional test sets. Scanning electron microscope (SEM) and atomic force microscopy (AFM) images showed that the surface of the reaction chamber was smoothly coated, illustrating the promising coating and isolating properties. Energy-dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), and inductively coupled plasma-optical emission spectrometer (ICP-OES) showed that no metal ions escaped from the metal to the chip surface. Fourier-transform infrared spectroscopy (FTIR) was used to check the surface chemical state before and after tests, and the unchanged infrared absorption peaks indicated the unreacted, antifouling surface. The limit of detection (LOD) of at least two copies can be obtained in this chip. |
| first_indexed | 2024-03-11T09:33:32Z |
| format | Article |
| id | doaj.art-904b209213ec4d439e574aa18d36d08c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T09:33:32Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-904b209213ec4d439e574aa18d36d08c2023-11-16T17:27:25ZengMDPI AGMolecules1420-30492023-01-01283108510.3390/molecules28031085An Aluminum-Based Microfluidic Chip for Polymerase Chain Reaction DiagnosisSiyu Yang0Ziyi Zhang1Qingyue Xian2Qi Song3Yiteng Liu4Yibo Gao5Weijia Wen6Division of Emerging Interdisciplinary Areas, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR 999077, ChinaDivision of Emerging Interdisciplinary Areas, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR 999077, ChinaDivision of Emerging Interdisciplinary Areas, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR 999077, ChinaDepartment of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR 999077, ChinaDivision of Emerging Interdisciplinary Areas, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR 999077, ChinaDepartment of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR 999077, ChinaDepartment of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR 999077, ChinaReal-time polymerase chain reaction (real-time PCR) tests were successfully conducted in an aluminum-based microfluidic chip developed in this work. The reaction chamber was coated with silicone-modified epoxy resin to isolate the reaction system from metal surfaces, preventing the metal ions from interfering with the reaction process. The patterned aluminum substrate was bonded with a hydroxylated glass mask using silicone sealant at room temperature. The effect of thermal expansion was counteracted by the elasticity of cured silicone. With the heating process closely monitored, real-time PCR testing in reaction chambers proceeded smoothly, and the results show similar quantification cycle values to those of traditional test sets. Scanning electron microscope (SEM) and atomic force microscopy (AFM) images showed that the surface of the reaction chamber was smoothly coated, illustrating the promising coating and isolating properties. Energy-dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), and inductively coupled plasma-optical emission spectrometer (ICP-OES) showed that no metal ions escaped from the metal to the chip surface. Fourier-transform infrared spectroscopy (FTIR) was used to check the surface chemical state before and after tests, and the unchanged infrared absorption peaks indicated the unreacted, antifouling surface. The limit of detection (LOD) of at least two copies can be obtained in this chip.https://www.mdpi.com/1420-3049/28/3/1085diagnosisreal-time polymerase chain reactionnucleic acid diagnosisaluminum chipsmicrofluidic chipsportability |
| spellingShingle | Siyu Yang Ziyi Zhang Qingyue Xian Qi Song Yiteng Liu Yibo Gao Weijia Wen An Aluminum-Based Microfluidic Chip for Polymerase Chain Reaction Diagnosis Molecules diagnosis real-time polymerase chain reaction nucleic acid diagnosis aluminum chips microfluidic chips portability |
| title | An Aluminum-Based Microfluidic Chip for Polymerase Chain Reaction Diagnosis |
| title_full | An Aluminum-Based Microfluidic Chip for Polymerase Chain Reaction Diagnosis |
| title_fullStr | An Aluminum-Based Microfluidic Chip for Polymerase Chain Reaction Diagnosis |
| title_full_unstemmed | An Aluminum-Based Microfluidic Chip for Polymerase Chain Reaction Diagnosis |
| title_short | An Aluminum-Based Microfluidic Chip for Polymerase Chain Reaction Diagnosis |
| title_sort | aluminum based microfluidic chip for polymerase chain reaction diagnosis |
| topic | diagnosis real-time polymerase chain reaction nucleic acid diagnosis aluminum chips microfluidic chips portability |
| url | https://www.mdpi.com/1420-3049/28/3/1085 |
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