Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids
It is well-known that two major issues, preventing improved outcomes from cancer are late diagnosis and the evolution of drug resistance during chemotherapy, therefore technologies that address these issues can have a transformative effect on healthcare workflows. In this work we present a simple, l...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/10/11/156 |
| _version_ | 1797549607369048064 |
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| author | Bukola Attoye Chantevy Pou Ewen Blair Christopher Rinaldi Fiona Thomson Matthew J. Baker Damion K. Corrigan |
| author_facet | Bukola Attoye Chantevy Pou Ewen Blair Christopher Rinaldi Fiona Thomson Matthew J. Baker Damion K. Corrigan |
| author_sort | Bukola Attoye |
| collection | DOAJ |
| description | It is well-known that two major issues, preventing improved outcomes from cancer are late diagnosis and the evolution of drug resistance during chemotherapy, therefore technologies that address these issues can have a transformative effect on healthcare workflows. In this work we present a simple, low-cost DNA biosensor that was developed specifically to detect mutations in a key oncogene (KRAS). The sensor employed was a screen-printed array of carbon electrodes, used to perform parallel measurements of DNA hybridisation. A DNA amplification reaction was developed with primers for mutant and wild type KRAS sequences which amplified target sequences from representative clinical samples to detectable levels in as few as twenty cycles. High levels of sensitivity were demonstrated alongside a clear exemplar of assay specificity by showing the mutant KRAS sequence was detectable against a significant background of wild type DNA following amplification and hybridisation on the sensor surface. The time to result was found to be 3.5 h with considerable potential for optimisation through assay integration. This quick and versatile biosensor has the potential to be deployed in a low-cost, point-of-care test where patients can be screened either for early diagnosis purposes or monitoring of response to therapy. |
| first_indexed | 2024-03-10T15:17:02Z |
| format | Article |
| id | doaj.art-904c7b8fb1534c46aec16b5a99ea04fc |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T15:17:02Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-904c7b8fb1534c46aec16b5a99ea04fc2023-11-20T18:50:33ZengMDPI AGBiosensors2079-63742020-10-01101115610.3390/bios10110156Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human FluidsBukola Attoye0Chantevy Pou1Ewen Blair2Christopher Rinaldi3Fiona Thomson4Matthew J. Baker5Damion K. Corrigan6Department of Biomedical Engineering, University of Strathclyde, 40 George Street, Glasgow G1 1QE, UKWolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Glasgow G61 1QH, UKDepartment of Biomedical Engineering, University of Strathclyde, 40 George Street, Glasgow G1 1QE, UKTechnology and Innovation Centre, Department of Pure and Applied Chemistry, University of Strathclyde, 99 George street, Glasgow G1 1RD, UKWolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Glasgow G61 1QH, UKTechnology and Innovation Centre, Department of Pure and Applied Chemistry, University of Strathclyde, 99 George street, Glasgow G1 1RD, UKDepartment of Biomedical Engineering, University of Strathclyde, 40 George Street, Glasgow G1 1QE, UKIt is well-known that two major issues, preventing improved outcomes from cancer are late diagnosis and the evolution of drug resistance during chemotherapy, therefore technologies that address these issues can have a transformative effect on healthcare workflows. In this work we present a simple, low-cost DNA biosensor that was developed specifically to detect mutations in a key oncogene (KRAS). The sensor employed was a screen-printed array of carbon electrodes, used to perform parallel measurements of DNA hybridisation. A DNA amplification reaction was developed with primers for mutant and wild type KRAS sequences which amplified target sequences from representative clinical samples to detectable levels in as few as twenty cycles. High levels of sensitivity were demonstrated alongside a clear exemplar of assay specificity by showing the mutant KRAS sequence was detectable against a significant background of wild type DNA following amplification and hybridisation on the sensor surface. The time to result was found to be 3.5 h with considerable potential for optimisation through assay integration. This quick and versatile biosensor has the potential to be deployed in a low-cost, point-of-care test where patients can be screened either for early diagnosis purposes or monitoring of response to therapy.https://www.mdpi.com/2079-6374/10/11/156electrochemicalDNA biosensorsKRASliquid biopsy |
| spellingShingle | Bukola Attoye Chantevy Pou Ewen Blair Christopher Rinaldi Fiona Thomson Matthew J. Baker Damion K. Corrigan Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids Biosensors electrochemical DNA biosensors KRAS liquid biopsy |
| title | Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids |
| title_full | Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids |
| title_fullStr | Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids |
| title_full_unstemmed | Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids |
| title_short | Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids |
| title_sort | developing a low cost simple to use electrochemical sensor for the detection of circulating tumour dna in human fluids |
| topic | electrochemical DNA biosensors KRAS liquid biopsy |
| url | https://www.mdpi.com/2079-6374/10/11/156 |
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