Role of complement and antibodies in controlling infection with pathogenic simian immunodeficiency virus (SIV) in macaques vaccinated with replication-deficient viral vectors
<p>Abstract</p> <p>Background</p> <p>We investigated the interplay between complement and antibodies upon priming with single-cycle replicating viral vectors (SCIV) encoding SIV antigens combined with Adeno5-SIV or SCIV pseudotyped with murine leukemia virus envelope bo...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-06-01
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| Series: | Retrovirology |
| Online Access: | http://www.retrovirology.com/content/6/1/60 |
| _version_ | 1818664963028287488 |
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| author | Strasak Alexander Norley Stephen Überla Klaus Kuate Seraphin Hennig Christiane Wilflingseder Doris Hiltgartner Alexander Rubner Barbara Falkensammer Barbara Racz Paul Stoiber Heribert |
| author_facet | Strasak Alexander Norley Stephen Überla Klaus Kuate Seraphin Hennig Christiane Wilflingseder Doris Hiltgartner Alexander Rubner Barbara Falkensammer Barbara Racz Paul Stoiber Heribert |
| author_sort | Strasak Alexander |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>We investigated the interplay between complement and antibodies upon priming with single-cycle replicating viral vectors (SCIV) encoding SIV antigens combined with Adeno5-SIV or SCIV pseudotyped with murine leukemia virus envelope boosting strategies. The vaccine was applied via spray-immunization to the tonsils of rhesus macaques and compared with systemic regimens.</p> <p>Results</p> <p>Independent of the application regimen or route, viral loads were significantly reduced after challenge with SIVmac239 (p < 0.03) compared to controls. Considerable amounts of neutralizing antibodies were induced in systemic immunized monkeys. Most of the sera harvested during peak viremia exhibited a trend with an inverse correlation between complement C3-deposition on viral particles and plasma viral load within the different vaccination groups. In contrast, the amount of the observed complement-mediated lysis did not correlate with the reduction of SIV titres.</p> <p>Conclusion</p> <p>The heterologous prime-boost strategy with replication-deficient viral vectors administered exclusively via the tonsils did not induce any neutralizing antibodies before challenge. However, after challenge, comparable SIV-specific humoral immune responses were observed in all vaccinated animals. Immunization with single cycle immunodeficiency viruses mounts humoral immune responses comparable to live-attenuated immunodeficiency virus vaccines.</p> |
| first_indexed | 2024-12-17T05:41:05Z |
| format | Article |
| id | doaj.art-904ce836d20a4dfc823ae73e53e9eaa0 |
| institution | Directory Open Access Journal |
| issn | 1742-4690 |
| language | English |
| last_indexed | 2024-12-17T05:41:05Z |
| publishDate | 2009-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Retrovirology |
| spelling | doaj.art-904ce836d20a4dfc823ae73e53e9eaa02022-12-21T22:01:26ZengBMCRetrovirology1742-46902009-06-01616010.1186/1742-4690-6-60Role of complement and antibodies in controlling infection with pathogenic simian immunodeficiency virus (SIV) in macaques vaccinated with replication-deficient viral vectorsStrasak AlexanderNorley StephenÜberla KlausKuate SeraphinHennig ChristianeWilflingseder DorisHiltgartner AlexanderRubner BarbaraFalkensammer BarbaraRacz PaulStoiber Heribert<p>Abstract</p> <p>Background</p> <p>We investigated the interplay between complement and antibodies upon priming with single-cycle replicating viral vectors (SCIV) encoding SIV antigens combined with Adeno5-SIV or SCIV pseudotyped with murine leukemia virus envelope boosting strategies. The vaccine was applied via spray-immunization to the tonsils of rhesus macaques and compared with systemic regimens.</p> <p>Results</p> <p>Independent of the application regimen or route, viral loads were significantly reduced after challenge with SIVmac239 (p < 0.03) compared to controls. Considerable amounts of neutralizing antibodies were induced in systemic immunized monkeys. Most of the sera harvested during peak viremia exhibited a trend with an inverse correlation between complement C3-deposition on viral particles and plasma viral load within the different vaccination groups. In contrast, the amount of the observed complement-mediated lysis did not correlate with the reduction of SIV titres.</p> <p>Conclusion</p> <p>The heterologous prime-boost strategy with replication-deficient viral vectors administered exclusively via the tonsils did not induce any neutralizing antibodies before challenge. However, after challenge, comparable SIV-specific humoral immune responses were observed in all vaccinated animals. Immunization with single cycle immunodeficiency viruses mounts humoral immune responses comparable to live-attenuated immunodeficiency virus vaccines.</p>http://www.retrovirology.com/content/6/1/60 |
| spellingShingle | Strasak Alexander Norley Stephen Überla Klaus Kuate Seraphin Hennig Christiane Wilflingseder Doris Hiltgartner Alexander Rubner Barbara Falkensammer Barbara Racz Paul Stoiber Heribert Role of complement and antibodies in controlling infection with pathogenic simian immunodeficiency virus (SIV) in macaques vaccinated with replication-deficient viral vectors Retrovirology |
| title | Role of complement and antibodies in controlling infection with pathogenic simian immunodeficiency virus (SIV) in macaques vaccinated with replication-deficient viral vectors |
| title_full | Role of complement and antibodies in controlling infection with pathogenic simian immunodeficiency virus (SIV) in macaques vaccinated with replication-deficient viral vectors |
| title_fullStr | Role of complement and antibodies in controlling infection with pathogenic simian immunodeficiency virus (SIV) in macaques vaccinated with replication-deficient viral vectors |
| title_full_unstemmed | Role of complement and antibodies in controlling infection with pathogenic simian immunodeficiency virus (SIV) in macaques vaccinated with replication-deficient viral vectors |
| title_short | Role of complement and antibodies in controlling infection with pathogenic simian immunodeficiency virus (SIV) in macaques vaccinated with replication-deficient viral vectors |
| title_sort | role of complement and antibodies in controlling infection with pathogenic simian immunodeficiency virus siv in macaques vaccinated with replication deficient viral vectors |
| url | http://www.retrovirology.com/content/6/1/60 |
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