The method to detect sIgE to thyroid peroxidase in cases of the patients with autoimmune and allergic diseases
Aim of this research has been to create a method to detect sIgE to thyroid peroxidase (TPO) in serum of the patients with autoimmune and allergic diseases.Materials and methods. In this research native affine-purified TPO (more the 95% clean, enzyme activity 60±15 of enzyme unit per mg) with immune...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2019-08-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.elpub.ru/jour/article/view/359 |
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| author | A. V. Zubkov N. S. Kuzmina S. A. Mazurina L. G. Butova N. M. Rahmatulina N. A. Sibgatulina V. B. Gervazieva |
| author_facet | A. V. Zubkov N. S. Kuzmina S. A. Mazurina L. G. Butova N. M. Rahmatulina N. A. Sibgatulina V. B. Gervazieva |
| author_sort | A. V. Zubkov |
| collection | DOAJ |
| description | Aim of this research has been to create a method to detect sIgE to thyroid peroxidase (TPO) in serum of the patients with autoimmune and allergic diseases.Materials and methods. In this research native affine-purified TPO (more the 95% clean, enzyme activity 60±15 of enzyme unit per mg) with immune dominant elements only (confirmed by inhibitory ELISA) has been used. The detection of sIgE in serum, combined with TPO, was carried out with monoclonal antibodies to IgE (8E/4F4) conjugated with horseradish peroxidase.Results. In serum of patients with bronchial asthma, allergic rhinitis and chronic spontaneous urticarial sIgE to TPO has been detected in 18—21% of cases. The healthy people and the patients with autoimmune thyroiditis had no sign of sIgE to TPO in their serum.Conclusion. The detection of sIgE to TPO in cases of patients with chronic spontaneous urticarial and allergic diseases will make possible to perform the early diagnosis of thyroid autoimmune disorders and provide timely and adequate therapy. |
| first_indexed | 2024-12-10T19:00:44Z |
| format | Article |
| id | doaj.art-904dcfdaba7d42fea72ee21e5b5f193a |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-12-10T19:00:44Z |
| publishDate | 2019-08-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-904dcfdaba7d42fea72ee21e5b5f193a2022-12-22T01:37:00ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132019-08-0103313610.36233/0372-9311-2019-3-31-36351The method to detect sIgE to thyroid peroxidase in cases of the patients with autoimmune and allergic diseasesA. V. Zubkov0N. S. Kuzmina1S. A. Mazurina2L. G. Butova3N. M. Rahmatulina4N. A. Sibgatulina5V. B. Gervazieva6Mechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraKazan State Medical AcademyKazan State Medical AcademyMechnikov Research Institute of Vaccines and SeraAim of this research has been to create a method to detect sIgE to thyroid peroxidase (TPO) in serum of the patients with autoimmune and allergic diseases.Materials and methods. In this research native affine-purified TPO (more the 95% clean, enzyme activity 60±15 of enzyme unit per mg) with immune dominant elements only (confirmed by inhibitory ELISA) has been used. The detection of sIgE in serum, combined with TPO, was carried out with monoclonal antibodies to IgE (8E/4F4) conjugated with horseradish peroxidase.Results. In serum of patients with bronchial asthma, allergic rhinitis and chronic spontaneous urticarial sIgE to TPO has been detected in 18—21% of cases. The healthy people and the patients with autoimmune thyroiditis had no sign of sIgE to TPO in their serum.Conclusion. The detection of sIgE to TPO in cases of patients with chronic spontaneous urticarial and allergic diseases will make possible to perform the early diagnosis of thyroid autoimmune disorders and provide timely and adequate therapy.https://microbiol.elpub.ru/jour/article/view/359sige to tpoallergic diseasesautoimmunitychronic spontaneous urticarial |
| spellingShingle | A. V. Zubkov N. S. Kuzmina S. A. Mazurina L. G. Butova N. M. Rahmatulina N. A. Sibgatulina V. B. Gervazieva The method to detect sIgE to thyroid peroxidase in cases of the patients with autoimmune and allergic diseases Журнал микробиологии, эпидемиологии и иммунобиологии sige to tpo allergic diseases autoimmunity chronic spontaneous urticarial |
| title | The method to detect sIgE to thyroid peroxidase in cases of the patients with autoimmune and allergic diseases |
| title_full | The method to detect sIgE to thyroid peroxidase in cases of the patients with autoimmune and allergic diseases |
| title_fullStr | The method to detect sIgE to thyroid peroxidase in cases of the patients with autoimmune and allergic diseases |
| title_full_unstemmed | The method to detect sIgE to thyroid peroxidase in cases of the patients with autoimmune and allergic diseases |
| title_short | The method to detect sIgE to thyroid peroxidase in cases of the patients with autoimmune and allergic diseases |
| title_sort | method to detect sige to thyroid peroxidase in cases of the patients with autoimmune and allergic diseases |
| topic | sige to tpo allergic diseases autoimmunity chronic spontaneous urticarial |
| url | https://microbiol.elpub.ru/jour/article/view/359 |
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