A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: an experimental study in vivo
Platelet-rich fibrin (PRF) is generated from the patients’ own venous blood by a single centrifugation step without the additional use of anticoagulants. Based on the previously described LSCC (low-speed centrifugation concept), our group showed that modification of the centrifugation setting, that...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-04-01
|
| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2018.1445835 |
| _version_ | 1797684278718365696 |
|---|---|
| author | Alica Kubesch Mike Barbeck Sarah Al-Maawi Anna Orlowska Patrick F. Booms Robert A. Sader Richard J. Miron Charles.J. Kirkpatrick Joseph Choukroun Shahram Ghanaati |
| author_facet | Alica Kubesch Mike Barbeck Sarah Al-Maawi Anna Orlowska Patrick F. Booms Robert A. Sader Richard J. Miron Charles.J. Kirkpatrick Joseph Choukroun Shahram Ghanaati |
| author_sort | Alica Kubesch |
| collection | DOAJ |
| description | Platelet-rich fibrin (PRF) is generated from the patients’ own venous blood by a single centrifugation step without the additional use of anticoagulants. Based on the previously described LSCC (low-speed centrifugation concept), our group showed that modification of the centrifugation setting, that is, reducing the relative centrifugal force (RCF) and mildly increasing the centrifugation time, resulted in modified solid and liquid PRF-matrices with increased number of platelets, leukocytes, and growth factors’ concentrations. The aim of this study was to determine whether RCF reduction might also result in different tissue reactions toward the two PRF-based matrices, especially vascularization and cell distribution in vivo. Two centrifugation protocols (PRF-high [719 g] and PRF-medium [222 g]) were compared in a subcutaneous implantation model of SCID mice at 5 and 10 days. Histological and histomorphometrical analyses were performed to quantify lymphocyte, neutrophil, human macrophage, and monocyte populations. CD31 was used to detect newly formed vessels, while all human cells were detected by using human vimentin as a pan-cellular marker. The results demonstrated that PRF-high elicited a dense and stable fibrin structure and prevented cellular penetration of the host tissue. By contrast, PRF-medium was more porous, had a significantly higher in vivo vascularization rate, and included significantly more human cells, especially at day 10, compared to PRF-high. These findings highlight the possibility of modifying the structure and composition of PRF matrices and thus selectively altering their regenerative potential in vivo. Clinical studies now must evaluate the different PRF matrices for bone and soft-tissue regeneration to validate possible benefits using personalized preparation protocols. |
| first_indexed | 2024-03-12T00:27:10Z |
| format | Article |
| id | doaj.art-904fbdc4d9204718986a5286526b4e7d |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:27:10Z |
| publishDate | 2019-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-904fbdc4d9204718986a5286526b4e7d2023-09-15T10:31:59ZengTaylor & Francis GroupPlatelets0953-71041369-16352019-04-0130332934010.1080/09537104.2018.14458351445835A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: an experimental study in vivoAlica Kubesch0Mike Barbeck1Sarah Al-Maawi2Anna Orlowska3Patrick F. Booms4Robert A. Sader5Richard J. Miron6Charles.J. Kirkpatrick7Joseph Choukroun8Shahram Ghanaati9University Hospital Frankfurt Goethe UniversityUniversity Hospital Frankfurt Goethe UniversityUniversity Hospital Frankfurt Goethe UniversityUniversity Hospital Frankfurt Goethe UniversityUniversity Hospital Frankfurt Goethe UniversityUniversity Hospital Frankfurt Goethe UniversityNova Southeastern UniversityUniversity Hospital Frankfurt Goethe UniversityUniversity Hospital Frankfurt Goethe UniversityUniversity Hospital Frankfurt Goethe UniversityPlatelet-rich fibrin (PRF) is generated from the patients’ own venous blood by a single centrifugation step without the additional use of anticoagulants. Based on the previously described LSCC (low-speed centrifugation concept), our group showed that modification of the centrifugation setting, that is, reducing the relative centrifugal force (RCF) and mildly increasing the centrifugation time, resulted in modified solid and liquid PRF-matrices with increased number of platelets, leukocytes, and growth factors’ concentrations. The aim of this study was to determine whether RCF reduction might also result in different tissue reactions toward the two PRF-based matrices, especially vascularization and cell distribution in vivo. Two centrifugation protocols (PRF-high [719 g] and PRF-medium [222 g]) were compared in a subcutaneous implantation model of SCID mice at 5 and 10 days. Histological and histomorphometrical analyses were performed to quantify lymphocyte, neutrophil, human macrophage, and monocyte populations. CD31 was used to detect newly formed vessels, while all human cells were detected by using human vimentin as a pan-cellular marker. The results demonstrated that PRF-high elicited a dense and stable fibrin structure and prevented cellular penetration of the host tissue. By contrast, PRF-medium was more porous, had a significantly higher in vivo vascularization rate, and included significantly more human cells, especially at day 10, compared to PRF-high. These findings highlight the possibility of modifying the structure and composition of PRF matrices and thus selectively altering their regenerative potential in vivo. Clinical studies now must evaluate the different PRF matrices for bone and soft-tissue regeneration to validate possible benefits using personalized preparation protocols.http://dx.doi.org/10.1080/09537104.2018.1445835drug deliveryinflammationlsccplatelet-rich fibrintissue engineeringvascularization |
| spellingShingle | Alica Kubesch Mike Barbeck Sarah Al-Maawi Anna Orlowska Patrick F. Booms Robert A. Sader Richard J. Miron Charles.J. Kirkpatrick Joseph Choukroun Shahram Ghanaati A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: an experimental study in vivo Platelets drug delivery inflammation lscc platelet-rich fibrin tissue engineering vascularization |
| title | A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: an experimental study in vivo |
| title_full | A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: an experimental study in vivo |
| title_fullStr | A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: an experimental study in vivo |
| title_full_unstemmed | A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: an experimental study in vivo |
| title_short | A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: an experimental study in vivo |
| title_sort | low speed centrifugation concept leads to cell accumulation and vascularization of solid platelet rich fibrin an experimental study in vivo |
| topic | drug delivery inflammation lscc platelet-rich fibrin tissue engineering vascularization |
| url | http://dx.doi.org/10.1080/09537104.2018.1445835 |
| work_keys_str_mv | AT alicakubesch alowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT mikebarbeck alowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT sarahalmaawi alowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT annaorlowska alowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT patrickfbooms alowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT robertasader alowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT richardjmiron alowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT charlesjkirkpatrick alowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT josephchoukroun alowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT shahramghanaati alowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT alicakubesch lowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT mikebarbeck lowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT sarahalmaawi lowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT annaorlowska lowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT patrickfbooms lowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT robertasader lowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT richardjmiron lowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT charlesjkirkpatrick lowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT josephchoukroun lowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo AT shahramghanaati lowspeedcentrifugationconceptleadstocellaccumulationandvascularizationofsolidplateletrichfibrinanexperimentalstudyinvivo |