Activity dynamics of amygdala GABAergic neurons during cataplexy of narcolepsy
Recent studies showed activation of the GABAergic neurons in the central nucleus of the amygdala (CeA) triggered cataplexy of sleep disorder narcolepsy. However, there is still no direct evidence on CeA GABAergic neurons’ real-time dynamic during cataplexy. We used a deep brain calcium imaging tool...
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| Format: | Article |
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eLife Sciences Publications Ltd
2019-08-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/48311 |
| _version_ | 1811235986125881344 |
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| author | Ying Sun Carlos Blanco-Centurion Emmaline Bendell Aurelio Vidal-Ortiz Siwei Luo Meng Liu |
| author_facet | Ying Sun Carlos Blanco-Centurion Emmaline Bendell Aurelio Vidal-Ortiz Siwei Luo Meng Liu |
| author_sort | Ying Sun |
| collection | DOAJ |
| description | Recent studies showed activation of the GABAergic neurons in the central nucleus of the amygdala (CeA) triggered cataplexy of sleep disorder narcolepsy. However, there is still no direct evidence on CeA GABAergic neurons’ real-time dynamic during cataplexy. We used a deep brain calcium imaging tool to image the intrinsic calcium transient as a marker of neuronal activity changes in the narcoleptic VGAT-Cre mice by expressing the calcium sensor GCaMP6 into genetically defined CeA GABAergic neurons. Two distinct GABAergic neuronal groups involved in cataplexy were identified: spontaneous cataplexy-ON and predator odor-induced cataplexy-ON neurons. Majority in the latter group were inactive during regular sleep/wake cycles but were specifically activated by predator odor and continued their intense activities into succeeding cataplexy bouts. Furthermore, we found that CeA GABAergic neurons became highly synchronized during predator odor-induced cataplexy. We suggest that the abnormal activation and synchronization of CeA GABAergic neurons may trigger emotion-induced cataplexy. |
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| id | doaj.art-9054db5d89604e3eaa20a24671be2381 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:01:31Z |
| publishDate | 2019-08-01 |
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| spelling | doaj.art-9054db5d89604e3eaa20a24671be23812022-12-22T03:33:50ZengeLife Sciences Publications LtdeLife2050-084X2019-08-01810.7554/eLife.48311Activity dynamics of amygdala GABAergic neurons during cataplexy of narcolepsyYing Sun0Carlos Blanco-Centurion1Emmaline Bendell2Aurelio Vidal-Ortiz3Siwei Luo4Meng Liu5https://orcid.org/0000-0003-1394-5014Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, United StatesDepartment of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, United StatesDepartment of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, United StatesDepartment of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, United StatesDepartment of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, United StatesDepartment of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, United StatesRecent studies showed activation of the GABAergic neurons in the central nucleus of the amygdala (CeA) triggered cataplexy of sleep disorder narcolepsy. However, there is still no direct evidence on CeA GABAergic neurons’ real-time dynamic during cataplexy. We used a deep brain calcium imaging tool to image the intrinsic calcium transient as a marker of neuronal activity changes in the narcoleptic VGAT-Cre mice by expressing the calcium sensor GCaMP6 into genetically defined CeA GABAergic neurons. Two distinct GABAergic neuronal groups involved in cataplexy were identified: spontaneous cataplexy-ON and predator odor-induced cataplexy-ON neurons. Majority in the latter group were inactive during regular sleep/wake cycles but were specifically activated by predator odor and continued their intense activities into succeeding cataplexy bouts. Furthermore, we found that CeA GABAergic neurons became highly synchronized during predator odor-induced cataplexy. We suggest that the abnormal activation and synchronization of CeA GABAergic neurons may trigger emotion-induced cataplexy.https://elifesciences.org/articles/48311cataplexyamygdalaGABA |
| spellingShingle | Ying Sun Carlos Blanco-Centurion Emmaline Bendell Aurelio Vidal-Ortiz Siwei Luo Meng Liu Activity dynamics of amygdala GABAergic neurons during cataplexy of narcolepsy eLife cataplexy amygdala GABA |
| title | Activity dynamics of amygdala GABAergic neurons during cataplexy of narcolepsy |
| title_full | Activity dynamics of amygdala GABAergic neurons during cataplexy of narcolepsy |
| title_fullStr | Activity dynamics of amygdala GABAergic neurons during cataplexy of narcolepsy |
| title_full_unstemmed | Activity dynamics of amygdala GABAergic neurons during cataplexy of narcolepsy |
| title_short | Activity dynamics of amygdala GABAergic neurons during cataplexy of narcolepsy |
| title_sort | activity dynamics of amygdala gabaergic neurons during cataplexy of narcolepsy |
| topic | cataplexy amygdala GABA |
| url | https://elifesciences.org/articles/48311 |
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