Metabolomic profile of patients on levothyroxine treatment for hypothyroidism
Background: Hypothyroidism is clinically characterized by a decrease in levels of the circulating thyroid hormones namely thyroxine and triiodothyronine. The main treatment for hypothyroidism is thyroid hormone replacement using levothyroxine to normalize serum thyroid hormone levels. Objectives:...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Bioscientifica
2023-08-01
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| Series: | European Thyroid Journal |
| Subjects: | |
| Online Access: | https://etj.bioscientifica.com/view/journals/etj/12/4/ETJ-23-0062.xml |
| _version_ | 1827879076308713472 |
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| author | Hicham Benabdelkamel Malak A Jaber Lina A Dahabiyeh Afshan Masood Reem H Almalki Mohthash Musambil Anas M Abdel Rahman Assim A Alfadda |
| author_facet | Hicham Benabdelkamel Malak A Jaber Lina A Dahabiyeh Afshan Masood Reem H Almalki Mohthash Musambil Anas M Abdel Rahman Assim A Alfadda |
| author_sort | Hicham Benabdelkamel |
| collection | DOAJ |
| description | Background: Hypothyroidism is clinically characterized by a decrease in levels of the circulating thyroid hormones namely thyroxine and triiodothyronine. The main treatment for hypothyroidism is thyroid hormone replacement using levothyroxine to normalize serum thyroid hormone levels.
Objectives: In this study, we explored the metabolic changes in the plasma of patients with hypothyroidism after reaching a euthyroid state with levothyroxine treatment.
Methods: Plasma samples from 18 patients diagnosed as overt hypothyroidism were collected before and after levothyroxine treatment upon reaching a euthyroid state and were analyzed by high-resolution mass spectrometry-based metabolomics. Multivariate and univariate analyses evaluated data to highlight potential metabolic biomarkers.
Results: Liquid chromatography-mass spectrometry-based metabolomics revealed a significant decrease in the levels of ceramide, phosphatidylch oline, triglycerides, acylcarnitine, and peptides after levothyroxine treatment; this could indicate a change in the fatty acid transportation system and an enhanced β-oxidation, compared with a hypothyroid state. At the same time, the decrease in the peptides suggested a shift in protein synthesis. In addition, there was a considerable rise in glycocholic acid following therapy, suggesting the involvement of thyroid hormones in stimulating bile acid production and secretion.
Conclusions: A metabolomic analysis of patients with hypothyroidism revealed significant changes in several metabolites and lipids after treatment. This study showed the value of the metabolomics technique in providing a complementary understanding of the pathophysiology of hypothyroidism and as a crucial tool for examining the molecular impact of levothyroxine treatment on hypothyroidism. It was an important tool for investigating the therapeutic effects of levothyroxine on hypothyroidism at the molecular level. |
| first_indexed | 2024-03-12T18:00:31Z |
| format | Article |
| id | doaj.art-9056839e2db6498c818cccb5fc78ee17 |
| institution | Directory Open Access Journal |
| issn | 2235-0802 |
| language | English |
| last_indexed | 2024-03-12T18:00:31Z |
| publishDate | 2023-08-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | European Thyroid Journal |
| spelling | doaj.art-9056839e2db6498c818cccb5fc78ee172023-08-02T11:34:07ZengBioscientificaEuropean Thyroid Journal2235-08022023-08-01124110https://doi.org/10.1530/ETJ-23-0062Metabolomic profile of patients on levothyroxine treatment for hypothyroidismHicham Benabdelkamel0Malak A Jaber1Lina A Dahabiyeh2Afshan Masood3Reem H Almalki4Mohthash Musambil5Anas M Abdel Rahman6Assim A Alfadda7Proteomics Resource Unit, Obesity Research Center, College of Medicine, King Saud University, Riyadh, Saudi ArabiaPharmaceutical Medicinal Chemistry & Pharmacognosy, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, JordanDivision of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman, JordanProteomics Resource Unit, Obesity Research Center, College of Medicine, King Saud University, Riyadh, Saudi ArabiaMetabolomics Section, Department of Clinical Genomics, Center for Genome Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaProteomics Resource Unit, Obesity Research Center, College of Medicine, King Saud University, Riyadh, Saudi ArabiaMetabolomics Section, Department of Clinical Genomics, Center for Genome Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia; Department of Biochemistry and Molecular Medicine, College of Medicine, Alfaisal University, Riyadh, Saudi Arabia Proteomics Resource Unit, Obesity Research Center, College of Medicine, King Saud University, Riyadh, Saudi Arabia; Department of Medicine, College of Medicine and King Saud Medical City, King Saud University, Riyadh, Saudi Arabia Background: Hypothyroidism is clinically characterized by a decrease in levels of the circulating thyroid hormones namely thyroxine and triiodothyronine. The main treatment for hypothyroidism is thyroid hormone replacement using levothyroxine to normalize serum thyroid hormone levels. Objectives: In this study, we explored the metabolic changes in the plasma of patients with hypothyroidism after reaching a euthyroid state with levothyroxine treatment. Methods: Plasma samples from 18 patients diagnosed as overt hypothyroidism were collected before and after levothyroxine treatment upon reaching a euthyroid state and were analyzed by high-resolution mass spectrometry-based metabolomics. Multivariate and univariate analyses evaluated data to highlight potential metabolic biomarkers. Results: Liquid chromatography-mass spectrometry-based metabolomics revealed a significant decrease in the levels of ceramide, phosphatidylch oline, triglycerides, acylcarnitine, and peptides after levothyroxine treatment; this could indicate a change in the fatty acid transportation system and an enhanced β-oxidation, compared with a hypothyroid state. At the same time, the decrease in the peptides suggested a shift in protein synthesis. In addition, there was a considerable rise in glycocholic acid following therapy, suggesting the involvement of thyroid hormones in stimulating bile acid production and secretion. Conclusions: A metabolomic analysis of patients with hypothyroidism revealed significant changes in several metabolites and lipids after treatment. This study showed the value of the metabolomics technique in providing a complementary understanding of the pathophysiology of hypothyroidism and as a crucial tool for examining the molecular impact of levothyroxine treatment on hypothyroidism. It was an important tool for investigating the therapeutic effects of levothyroxine on hypothyroidism at the molecular level.https://etj.bioscientifica.com/view/journals/etj/12/4/ETJ-23-0062.xmlthyroid hormonehypothyroidismlc-ms based metabolomicslevothyroxinelipid disturbanceanabolic effectbile acidmetabolomics biomarker |
| spellingShingle | Hicham Benabdelkamel Malak A Jaber Lina A Dahabiyeh Afshan Masood Reem H Almalki Mohthash Musambil Anas M Abdel Rahman Assim A Alfadda Metabolomic profile of patients on levothyroxine treatment for hypothyroidism European Thyroid Journal thyroid hormone hypothyroidism lc-ms based metabolomics levothyroxine lipid disturbance anabolic effect bile acid metabolomics biomarker |
| title | Metabolomic profile of patients on levothyroxine treatment for hypothyroidism |
| title_full | Metabolomic profile of patients on levothyroxine treatment for hypothyroidism |
| title_fullStr | Metabolomic profile of patients on levothyroxine treatment for hypothyroidism |
| title_full_unstemmed | Metabolomic profile of patients on levothyroxine treatment for hypothyroidism |
| title_short | Metabolomic profile of patients on levothyroxine treatment for hypothyroidism |
| title_sort | metabolomic profile of patients on levothyroxine treatment for hypothyroidism |
| topic | thyroid hormone hypothyroidism lc-ms based metabolomics levothyroxine lipid disturbance anabolic effect bile acid metabolomics biomarker |
| url | https://etj.bioscientifica.com/view/journals/etj/12/4/ETJ-23-0062.xml |
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