Connexin Signaling in the Juxtaglomerular Apparatus (JGA) of Developing, Postnatal Healthy and Nephrotic Human Kidneys
Our study analyzed the expression pattern of different connexins (Cxs) and renin positive cells in the juxtaglomerular apparatus (JGA) of developing, postnatal healthy human kidneys and in nephrotic syndrome of the Finnish type (CNF), by using double immunofluorescence, electron microscopy and stati...
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MDPI AG
2020-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/21/8349 |
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| author | Ivona Kosovic Natalija Filipovic Benjamin Benzon Ivana Bocina Merica Glavina Durdov Katarina Vukojevic Marijan Saraga Mirna Saraga-Babic |
| author_facet | Ivona Kosovic Natalija Filipovic Benjamin Benzon Ivana Bocina Merica Glavina Durdov Katarina Vukojevic Marijan Saraga Mirna Saraga-Babic |
| author_sort | Ivona Kosovic |
| collection | DOAJ |
| description | Our study analyzed the expression pattern of different connexins (Cxs) and renin positive cells in the juxtaglomerular apparatus (JGA) of developing, postnatal healthy human kidneys and in nephrotic syndrome of the Finnish type (CNF), by using double immunofluorescence, electron microscopy and statistical measuring. The JGA contained several cell types connected by Cxs, and consisting of macula densa, extraglomerular mesangium (EM) and juxtaglomerular cells (JC), which release renin involved in renin-angiotensin- aldosteron system (RAS) of arterial blood pressure control. During JGA development, strong Cx40 expression gradually decreased, while expression of Cx37, Cx43 and Cx45 increased, postnatally showing more equalized expression patterning. In parallel, initially dispersed renin cells localized to JGA, and greatly increased expression in postnatal kidneys. In CNF kidneys, increased levels of Cx43, Cx37 and Cx45 co-localized with accumulations of renin cells in JGA. Additionally, they reappeared in extraglomerular mesangial cells, indicating association between return to embryonic Cxs patterning and pathologically changed kidney tissue. Based on the described Cxs and renin expression patterning, we suggest involvement of Cx40 primarily in the formation of JGA in developing kidneys, while Cx37, Cx43 and Cx45 might participate in JGA signal transfer important for postnatal maintenance of kidney function and blood pressure control. |
| first_indexed | 2024-03-10T15:02:17Z |
| format | Article |
| id | doaj.art-90568e6397124c7a87a51b88d6789c4c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T15:02:17Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-90568e6397124c7a87a51b88d6789c4c2023-11-20T20:05:35ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012121834910.3390/ijms21218349Connexin Signaling in the Juxtaglomerular Apparatus (JGA) of Developing, Postnatal Healthy and Nephrotic Human KidneysIvona Kosovic0Natalija Filipovic1Benjamin Benzon2Ivana Bocina3Merica Glavina Durdov4Katarina Vukojevic5Marijan Saraga6Mirna Saraga-Babic7Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, 21000 Split, CroatiaDepartment of Anatomy, Histology and Embryology, School of Medicine, University of Split, 21000 Split, CroatiaDepartment of Anatomy, Histology and Embryology, School of Medicine, University of Split, 21000 Split, CroatiaDepartment of Biology, Faculty of Science, University of Split, 21000 Split, CroatiaDepartment of Pathology, University Hospital in Split, School of Medicine, University of Split, 21000 Split, CroatiaDepartment of Anatomy, Histology and Embryology, School of Medicine, University of Split, 21000 Split, CroatiaDepartment of Paediatrics, University Hospital in Split, School of Medicine, University of Split, 21000 Split, CroatiaDepartment of Anatomy, Histology and Embryology, School of Medicine, University of Split, 21000 Split, CroatiaOur study analyzed the expression pattern of different connexins (Cxs) and renin positive cells in the juxtaglomerular apparatus (JGA) of developing, postnatal healthy human kidneys and in nephrotic syndrome of the Finnish type (CNF), by using double immunofluorescence, electron microscopy and statistical measuring. The JGA contained several cell types connected by Cxs, and consisting of macula densa, extraglomerular mesangium (EM) and juxtaglomerular cells (JC), which release renin involved in renin-angiotensin- aldosteron system (RAS) of arterial blood pressure control. During JGA development, strong Cx40 expression gradually decreased, while expression of Cx37, Cx43 and Cx45 increased, postnatally showing more equalized expression patterning. In parallel, initially dispersed renin cells localized to JGA, and greatly increased expression in postnatal kidneys. In CNF kidneys, increased levels of Cx43, Cx37 and Cx45 co-localized with accumulations of renin cells in JGA. Additionally, they reappeared in extraglomerular mesangial cells, indicating association between return to embryonic Cxs patterning and pathologically changed kidney tissue. Based on the described Cxs and renin expression patterning, we suggest involvement of Cx40 primarily in the formation of JGA in developing kidneys, while Cx37, Cx43 and Cx45 might participate in JGA signal transfer important for postnatal maintenance of kidney function and blood pressure control.https://www.mdpi.com/1422-0067/21/21/8349kidney developmentCNFconnexinsreninblood pressure control |
| spellingShingle | Ivona Kosovic Natalija Filipovic Benjamin Benzon Ivana Bocina Merica Glavina Durdov Katarina Vukojevic Marijan Saraga Mirna Saraga-Babic Connexin Signaling in the Juxtaglomerular Apparatus (JGA) of Developing, Postnatal Healthy and Nephrotic Human Kidneys International Journal of Molecular Sciences kidney development CNF connexins renin blood pressure control |
| title | Connexin Signaling in the Juxtaglomerular Apparatus (JGA) of Developing, Postnatal Healthy and Nephrotic Human Kidneys |
| title_full | Connexin Signaling in the Juxtaglomerular Apparatus (JGA) of Developing, Postnatal Healthy and Nephrotic Human Kidneys |
| title_fullStr | Connexin Signaling in the Juxtaglomerular Apparatus (JGA) of Developing, Postnatal Healthy and Nephrotic Human Kidneys |
| title_full_unstemmed | Connexin Signaling in the Juxtaglomerular Apparatus (JGA) of Developing, Postnatal Healthy and Nephrotic Human Kidneys |
| title_short | Connexin Signaling in the Juxtaglomerular Apparatus (JGA) of Developing, Postnatal Healthy and Nephrotic Human Kidneys |
| title_sort | connexin signaling in the juxtaglomerular apparatus jga of developing postnatal healthy and nephrotic human kidneys |
| topic | kidney development CNF connexins renin blood pressure control |
| url | https://www.mdpi.com/1422-0067/21/21/8349 |
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