Immunohistochemical Detection of BRAFV600E in Cutaneous Melanocytic Neoplasms in a Cohort of Egyptian Population
Objective: Melanocytic neoplasia shows different pathways that have been implicated in tumorigenesis. One of the key genes involved in the BRAFV600E gene, can be detected through DNA-based or protein-based tests including immunohistochemistry (IHC). To check its reproducibility, this study was condu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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West Asia Organization for Cancer Prevention
2022-12-01
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| Series: | Asian Pacific Journal of Cancer Care |
| Subjects: | |
| Online Access: | http://www.waocp.com/journal/index.php/apjcc/article/view/925 |
| _version_ | 1827370921000697856 |
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| author | Nada M. Yakout Dina M Abdallah Doaa A Abdelmonsif Hassan Mahmoud Kholosy Omayma Elsakka |
| author_facet | Nada M. Yakout Dina M Abdallah Doaa A Abdelmonsif Hassan Mahmoud Kholosy Omayma Elsakka |
| author_sort | Nada M. Yakout |
| collection | DOAJ |
| description | Objective: Melanocytic neoplasia shows different pathways that have been implicated in tumorigenesis. One of the key genes involved in the BRAFV600E gene, can be detected through DNA-based or protein-based tests including immunohistochemistry (IHC). To check its reproducibility, this study was conducted to evaluate BRAFV600E IHC by different observers in a range of melanocytic neoplasms.
Methods: The present study was performed to evaluate immunohistochemical staining (IHC) using the RM-08 to detect BRAF V600E in 50 formalin-fixed paraffin-embedded (FFPE) blocks of different melanocytic neoplasms after bleaching using Tris-HCL buffer. IHC was considered positive when more than 90% of tumour cells showed cytoplasmic staining. IHC assessment by a team of two pathologists and a third pathologist to check the interobserver variability. IHC scores were compared among nevi and melanoma.
Results: IHC evaluation revealed good agreement by different observers (ĸ-coefficient=0.691, p=0.00). BRAFV600E showed a statistically significant difference between nevi and melanoma.
Conclusions: These findings suggest that BRAFV600E IHC assessment shows good reproducibility among pathologists, however, more strict criteria should be adopted in interpretation, especially in bleached samples. |
| first_indexed | 2024-03-08T10:30:37Z |
| format | Article |
| id | doaj.art-9056a743e7f9430aa46eea32d3536efa |
| institution | Directory Open Access Journal |
| issn | 2588-3682 |
| language | English |
| last_indexed | 2024-03-08T10:30:37Z |
| publishDate | 2022-12-01 |
| publisher | West Asia Organization for Cancer Prevention |
| record_format | Article |
| series | Asian Pacific Journal of Cancer Care |
| spelling | doaj.art-9056a743e7f9430aa46eea32d3536efa2024-01-27T05:57:20ZengWest Asia Organization for Cancer PreventionAsian Pacific Journal of Cancer Care2588-36822022-12-017474775110.31557/apjcc.2022.7.4.747-751925Immunohistochemical Detection of BRAFV600E in Cutaneous Melanocytic Neoplasms in a Cohort of Egyptian PopulationNada M. Yakout0Dina M Abdallah1Doaa A Abdelmonsif2Hassan Mahmoud Kholosy3Omayma Elsakka4Pathology Department, Faculty of Medicine, Alexandria University, Egypt.Pathology Department, Faculty of Medicine, Alexandria University, Egypt.Medical Biochemistry Department, Genomics Research Lab, Faculty of Medicine, Alexandria University, Egypt.Department of Plastic Surgery, Faculty of Medicine, Alexandria University, Egypt.Pathology Department, Faculty of Medicine, Alexandria University, Egypt.Objective: Melanocytic neoplasia shows different pathways that have been implicated in tumorigenesis. One of the key genes involved in the BRAFV600E gene, can be detected through DNA-based or protein-based tests including immunohistochemistry (IHC). To check its reproducibility, this study was conducted to evaluate BRAFV600E IHC by different observers in a range of melanocytic neoplasms. Methods: The present study was performed to evaluate immunohistochemical staining (IHC) using the RM-08 to detect BRAF V600E in 50 formalin-fixed paraffin-embedded (FFPE) blocks of different melanocytic neoplasms after bleaching using Tris-HCL buffer. IHC was considered positive when more than 90% of tumour cells showed cytoplasmic staining. IHC assessment by a team of two pathologists and a third pathologist to check the interobserver variability. IHC scores were compared among nevi and melanoma. Results: IHC evaluation revealed good agreement by different observers (ĸ-coefficient=0.691, p=0.00). BRAFV600E showed a statistically significant difference between nevi and melanoma. Conclusions: These findings suggest that BRAFV600E IHC assessment shows good reproducibility among pathologists, however, more strict criteria should be adopted in interpretation, especially in bleached samples.http://www.waocp.com/journal/index.php/apjcc/article/view/925brafv600emelanomanevibleachingegyptian |
| spellingShingle | Nada M. Yakout Dina M Abdallah Doaa A Abdelmonsif Hassan Mahmoud Kholosy Omayma Elsakka Immunohistochemical Detection of BRAFV600E in Cutaneous Melanocytic Neoplasms in a Cohort of Egyptian Population Asian Pacific Journal of Cancer Care brafv600e melanoma nevi bleaching egyptian |
| title | Immunohistochemical Detection of BRAFV600E in Cutaneous Melanocytic Neoplasms in a Cohort of Egyptian Population |
| title_full | Immunohistochemical Detection of BRAFV600E in Cutaneous Melanocytic Neoplasms in a Cohort of Egyptian Population |
| title_fullStr | Immunohistochemical Detection of BRAFV600E in Cutaneous Melanocytic Neoplasms in a Cohort of Egyptian Population |
| title_full_unstemmed | Immunohistochemical Detection of BRAFV600E in Cutaneous Melanocytic Neoplasms in a Cohort of Egyptian Population |
| title_short | Immunohistochemical Detection of BRAFV600E in Cutaneous Melanocytic Neoplasms in a Cohort of Egyptian Population |
| title_sort | immunohistochemical detection of brafv600e in cutaneous melanocytic neoplasms in a cohort of egyptian population |
| topic | brafv600e melanoma nevi bleaching egyptian |
| url | http://www.waocp.com/journal/index.php/apjcc/article/view/925 |
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