The transcriptome sequencing analysis reveals immune mechanisms of soybean fermented powder on the loach (Misgurnus anguillicaudatus) in response to Lipopolysaccharide (LPS) infection
The loach (Misgurnus anguillicaudatus), a small commercial fish that is widely cultivated for its high-quality protein, vitamins, minerals, and essential amino acid, is a member of the genus Misgurnus and the family Cyprinidae. In this study, we gave the LPS-injected loach fermented soybean meal and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1247038/full |
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| author | Yayuan Xu Yayuan Xu Xinxin Zhang Dongqi Li Dongqi Li Kun Qian Kun Qian Yu Liu Tingjuan Xu Lishang Dai Jianghua Cheng Jianghua Cheng |
| author_facet | Yayuan Xu Yayuan Xu Xinxin Zhang Dongqi Li Dongqi Li Kun Qian Kun Qian Yu Liu Tingjuan Xu Lishang Dai Jianghua Cheng Jianghua Cheng |
| author_sort | Yayuan Xu |
| collection | DOAJ |
| description | The loach (Misgurnus anguillicaudatus), a small commercial fish that is widely cultivated for its high-quality protein, vitamins, minerals, and essential amino acid, is a member of the genus Misgurnus and the family Cyprinidae. In this study, we gave the LPS-injected loach fermented soybean meal and used transcriptome sequencing to investigate the impact of the fermented soybean powder on the loach’s immune system. 3384 up-regulated genes and 12116 down-regulated genes were found among the 15500 differentially expressed genes, according to the results. The differentially expressed genes were shown to be involved in cellular processes, metabolic processes, cellular anatomical entities, and binding, according to the Go functional annotation. Meanwhile, the KEGG enrichment analysis indicated that the soybean fermented powder treated groups showed significant differences in DNA replication, Nucleotide excision repair, Fanconi anemia pathway, and Base excision repair pathways, suggesting that these pathways are closely related to the enhancement of the immune function of loach by soybean fermented powder. The particular conclusions not exclusively can provide a new conception for the rational utilization of soybean fermented powder but also can provide theoretical guidance for the subsequent healthy breeding of loach. |
| first_indexed | 2024-03-12T14:24:27Z |
| format | Article |
| id | doaj.art-905752ae0bf14d5b95be233d8265ca7c |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-12T14:24:27Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-905752ae0bf14d5b95be233d8265ca7c2023-08-18T11:12:51ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-08-011410.3389/fimmu.2023.12470381247038The transcriptome sequencing analysis reveals immune mechanisms of soybean fermented powder on the loach (Misgurnus anguillicaudatus) in response to Lipopolysaccharide (LPS) infectionYayuan Xu0Yayuan Xu1Xinxin Zhang2Dongqi Li3Dongqi Li4Kun Qian5Kun Qian6Yu Liu7Tingjuan Xu8Lishang Dai9Jianghua Cheng10Jianghua Cheng11Institute of Agro-Products Processing, Anhui Academy of Agricultural Sciences, Hefei, ChinaAnhui Engineering Laboratory of Food Microbial Fermentation and Functional Application, Hefei, ChinaSchool of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Agro-Products Processing, Anhui Academy of Agricultural Sciences, Hefei, ChinaAnhui Engineering Laboratory of Food Microbial Fermentation and Functional Application, Hefei, ChinaInstitute of Agro-Products Processing, Anhui Academy of Agricultural Sciences, Hefei, ChinaAnhui Engineering Laboratory of Food Microbial Fermentation and Functional Application, Hefei, ChinaSchool of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, ChinaGerontology Institute, The First Affiliated Hospital of University of Science and Technology of China (USTC), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, ChinaSchool of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, ChinaInstitute of Agro-Products Processing, Anhui Academy of Agricultural Sciences, Hefei, ChinaAnhui Engineering Laboratory of Food Microbial Fermentation and Functional Application, Hefei, ChinaThe loach (Misgurnus anguillicaudatus), a small commercial fish that is widely cultivated for its high-quality protein, vitamins, minerals, and essential amino acid, is a member of the genus Misgurnus and the family Cyprinidae. In this study, we gave the LPS-injected loach fermented soybean meal and used transcriptome sequencing to investigate the impact of the fermented soybean powder on the loach’s immune system. 3384 up-regulated genes and 12116 down-regulated genes were found among the 15500 differentially expressed genes, according to the results. The differentially expressed genes were shown to be involved in cellular processes, metabolic processes, cellular anatomical entities, and binding, according to the Go functional annotation. Meanwhile, the KEGG enrichment analysis indicated that the soybean fermented powder treated groups showed significant differences in DNA replication, Nucleotide excision repair, Fanconi anemia pathway, and Base excision repair pathways, suggesting that these pathways are closely related to the enhancement of the immune function of loach by soybean fermented powder. The particular conclusions not exclusively can provide a new conception for the rational utilization of soybean fermented powder but also can provide theoretical guidance for the subsequent healthy breeding of loach.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1247038/fulltranscriptome sequencingsoybean fermentation powderloachLPSimmune function |
| spellingShingle | Yayuan Xu Yayuan Xu Xinxin Zhang Dongqi Li Dongqi Li Kun Qian Kun Qian Yu Liu Tingjuan Xu Lishang Dai Jianghua Cheng Jianghua Cheng The transcriptome sequencing analysis reveals immune mechanisms of soybean fermented powder on the loach (Misgurnus anguillicaudatus) in response to Lipopolysaccharide (LPS) infection Frontiers in Immunology transcriptome sequencing soybean fermentation powder loach LPS immune function |
| title | The transcriptome sequencing analysis reveals immune mechanisms of soybean fermented powder on the loach (Misgurnus anguillicaudatus) in response to Lipopolysaccharide (LPS) infection |
| title_full | The transcriptome sequencing analysis reveals immune mechanisms of soybean fermented powder on the loach (Misgurnus anguillicaudatus) in response to Lipopolysaccharide (LPS) infection |
| title_fullStr | The transcriptome sequencing analysis reveals immune mechanisms of soybean fermented powder on the loach (Misgurnus anguillicaudatus) in response to Lipopolysaccharide (LPS) infection |
| title_full_unstemmed | The transcriptome sequencing analysis reveals immune mechanisms of soybean fermented powder on the loach (Misgurnus anguillicaudatus) in response to Lipopolysaccharide (LPS) infection |
| title_short | The transcriptome sequencing analysis reveals immune mechanisms of soybean fermented powder on the loach (Misgurnus anguillicaudatus) in response to Lipopolysaccharide (LPS) infection |
| title_sort | transcriptome sequencing analysis reveals immune mechanisms of soybean fermented powder on the loach misgurnus anguillicaudatus in response to lipopolysaccharide lps infection |
| topic | transcriptome sequencing soybean fermentation powder loach LPS immune function |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1247038/full |
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