Characterization and hepatoprotective evaluation of sesquiterpenes and diterpenes from the aerial parts of Juniperus sabina L.
Previously, we reported on the hepatoprotective activity of the total extract of Juniperus sabina L. against CCl4 induced liver toxicity in experimental animals. Biologically directed phytochemical study was conducted to identify the active compounds. Male Wistar rats and the standard drug silymarin...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-11-01
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| Series: | Saudi Pharmaceutical Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016419300921 |
| _version_ | 1818925065721348096 |
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| author | Maged S. Abdel-Kader Abubaker M. Hamad Mubarak T. Alanazi Abdullah H. Alanazi Rizwan Ali Ahmed I. Foudah Mohammed H. Alqarni |
| author_facet | Maged S. Abdel-Kader Abubaker M. Hamad Mubarak T. Alanazi Abdullah H. Alanazi Rizwan Ali Ahmed I. Foudah Mohammed H. Alqarni |
| author_sort | Maged S. Abdel-Kader |
| collection | DOAJ |
| description | Previously, we reported on the hepatoprotective activity of the total extract of Juniperus sabina L. against CCl4 induced liver toxicity in experimental animals. Biologically directed phytochemical study was conducted to identify the active compounds. Male Wistar rats and the standard drug silymarin were used in the study. Hepatoprotective activity was evaluated via serum biochemical parameters such as aspartate amino transferase (AST), alanine aminotransferase (ALT), gamma glutamyl transpeptidase (GGT), alkaline phosphatase (ALP) and total bilirubin. Tissue parameters including non-protein sulfhydryl groups (NP-SH), malonaldehyde (MDA) and total protein (TP) were also determined. Histopathological study was conducted utilizing Mayer’s hematoxylin stain, Periodic Acid Schiff – Hematoxylin (PAS-H) and Masson trichrome technique on light microscope. Electron microscope images were also generated for the study. The activity of the total extract was trapped to the petroleum ether fraction after liquid-liquid fractionation where 51% reduction in the levels of AST, bilirubin and 44% in the levels of ALT were observed. Chromatographic purification of the petroleum ether fraction resulted in the isolation of nine compounds namely: trans-calamenene (1), cadalene (cadalin) (2), epi-cubenol (3), manool (4), calamenene-10β-ol (5), calamenene-10α-ol (6), 4-epi-abietic acid (7), sandaracopimaric acid (8) and isopimaric acid (9). Compounds 1–3, 5 and 6 are belonging to cadinane sesquiterepenes, while compounds 4, 7–9 were of diterpene skeleton. The major compounds were tested for their hepatoprotective effect. Compounds 3 showed marked improvement in the levels of AST and ALT, compound 4 was effective in improving the levels of AST, ALT, GGT, ALP and bilirubin, while compound 7 showed significant improvement in GGT, ALP and bilirubin levels. Keywords: Juniperus sabina, Hepatoprotective, Sesquiterpenes, Diterpenes, Electron microscope |
| first_indexed | 2024-12-20T02:35:18Z |
| format | Article |
| id | doaj.art-905ad0b4692241b99207cf087b065a86 |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-12-20T02:35:18Z |
| publishDate | 2019-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-905ad0b4692241b99207cf087b065a862022-12-21T19:56:27ZengElsevierSaudi Pharmaceutical Journal1319-01642019-11-01277920929Characterization and hepatoprotective evaluation of sesquiterpenes and diterpenes from the aerial parts of Juniperus sabina L.Maged S. Abdel-Kader0Abubaker M. Hamad1Mubarak T. Alanazi2Abdullah H. Alanazi3Rizwan Ali4Ahmed I. Foudah5Mohammed H. Alqarni6Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia; Department of Pharmacognosy, College of Pharmacy, Alexandria University, Alexandria 21215, Egypt; Corresponding author at: Department of Pharmacognosy, College of Pharmacy, Prince Sattam, Bin Abdulaziz University, 11942 Alkharj, KSA, Saudi Arabia.Basic Sciences Department, Preparatory year Deanship, Prince Sattam Bin Abdulaziz University. PO Box 20337, Alkharj 11942, Saudi Arabia; Department of Histopathology and Cytopathology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Madani, SudanPfizer Saudi Limited Pharmaceutical Company, P.O. Box 6722, Riyadh 11452, Saudi ArabiaDepartment of Pathology and Laboratory Medicine, Ministry of the National Guard - Health Affairs, Riyadh 11426, Saudi ArabiaDepartment of Pathology and Laboratory Medicine, Ministry of the National Guard - Health Affairs, Riyadh 11426, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi ArabiaPreviously, we reported on the hepatoprotective activity of the total extract of Juniperus sabina L. against CCl4 induced liver toxicity in experimental animals. Biologically directed phytochemical study was conducted to identify the active compounds. Male Wistar rats and the standard drug silymarin were used in the study. Hepatoprotective activity was evaluated via serum biochemical parameters such as aspartate amino transferase (AST), alanine aminotransferase (ALT), gamma glutamyl transpeptidase (GGT), alkaline phosphatase (ALP) and total bilirubin. Tissue parameters including non-protein sulfhydryl groups (NP-SH), malonaldehyde (MDA) and total protein (TP) were also determined. Histopathological study was conducted utilizing Mayer’s hematoxylin stain, Periodic Acid Schiff – Hematoxylin (PAS-H) and Masson trichrome technique on light microscope. Electron microscope images were also generated for the study. The activity of the total extract was trapped to the petroleum ether fraction after liquid-liquid fractionation where 51% reduction in the levels of AST, bilirubin and 44% in the levels of ALT were observed. Chromatographic purification of the petroleum ether fraction resulted in the isolation of nine compounds namely: trans-calamenene (1), cadalene (cadalin) (2), epi-cubenol (3), manool (4), calamenene-10β-ol (5), calamenene-10α-ol (6), 4-epi-abietic acid (7), sandaracopimaric acid (8) and isopimaric acid (9). Compounds 1–3, 5 and 6 are belonging to cadinane sesquiterepenes, while compounds 4, 7–9 were of diterpene skeleton. The major compounds were tested for their hepatoprotective effect. Compounds 3 showed marked improvement in the levels of AST and ALT, compound 4 was effective in improving the levels of AST, ALT, GGT, ALP and bilirubin, while compound 7 showed significant improvement in GGT, ALP and bilirubin levels. Keywords: Juniperus sabina, Hepatoprotective, Sesquiterpenes, Diterpenes, Electron microscopehttp://www.sciencedirect.com/science/article/pii/S1319016419300921 |
| spellingShingle | Maged S. Abdel-Kader Abubaker M. Hamad Mubarak T. Alanazi Abdullah H. Alanazi Rizwan Ali Ahmed I. Foudah Mohammed H. Alqarni Characterization and hepatoprotective evaluation of sesquiterpenes and diterpenes from the aerial parts of Juniperus sabina L. Saudi Pharmaceutical Journal |
| title | Characterization and hepatoprotective evaluation of sesquiterpenes and diterpenes from the aerial parts of Juniperus sabina L. |
| title_full | Characterization and hepatoprotective evaluation of sesquiterpenes and diterpenes from the aerial parts of Juniperus sabina L. |
| title_fullStr | Characterization and hepatoprotective evaluation of sesquiterpenes and diterpenes from the aerial parts of Juniperus sabina L. |
| title_full_unstemmed | Characterization and hepatoprotective evaluation of sesquiterpenes and diterpenes from the aerial parts of Juniperus sabina L. |
| title_short | Characterization and hepatoprotective evaluation of sesquiterpenes and diterpenes from the aerial parts of Juniperus sabina L. |
| title_sort | characterization and hepatoprotective evaluation of sesquiterpenes and diterpenes from the aerial parts of juniperus sabina l |
| url | http://www.sciencedirect.com/science/article/pii/S1319016419300921 |
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