Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens
ABSTRACT: Protozoa of the genus Phytomonas are harmful parasites to several agricultural crops of economic importance. Due to their recognized biological activity, crude extracts of Piper aduncum, P. crassinervium, P. hispidum, and P. amalago leaves, were tested using the microdilution plate techniq...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Santa Maria
2020-08-01
|
| Series: | Ciência Rural |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001000152&tlng=en |
| _version_ | 1818976945469128704 |
|---|---|
| author | Neriani de Souza Cancini Jesieli Beraldo-Borrazzo Jéssica Lima de Menezes Diógenes Aparício Garcia Cortez Rodrigo Hinojosa Valdez Francielle Pelegrin Garcia Sueli Fumie Yamada-Ogatta Benedito Prado Dias Filho Tânia Ueda-Nakamura Celso Vataru Nakamura Benício Alves de Abreu Filho |
| author_facet | Neriani de Souza Cancini Jesieli Beraldo-Borrazzo Jéssica Lima de Menezes Diógenes Aparício Garcia Cortez Rodrigo Hinojosa Valdez Francielle Pelegrin Garcia Sueli Fumie Yamada-Ogatta Benedito Prado Dias Filho Tânia Ueda-Nakamura Celso Vataru Nakamura Benício Alves de Abreu Filho |
| author_sort | Neriani de Souza Cancini |
| collection | DOAJ |
| description | ABSTRACT: Protozoa of the genus Phytomonas are harmful parasites to several agricultural crops of economic importance. Due to their recognized biological activity, crude extracts of Piper aduncum, P. crassinervium, P. hispidum, and P. amalago leaves, were tested using the microdilution plate technique to assess the antiparasitic potential against Phytomonas serpens. Results showed that the ethanolic crude extract of P. crassinervium and P. amalago presented the best inhibitory concentration for 50% of the cells (IC50), 16.5 µg mL-1 in chloroform phase, and 18 µg mL-1 in aqueous phase, respectively, after 48 h treatment. Cytotoxicity analyses were performed using the colorimetric method of sulforhodamine-B in LLCMK2 mammalian cells. The chloroform phase of P. crassinervium was subjected to the fractionation process, in which the ethyl acetate and dichloromethane fractions obtained better IC50 values. Scanning electron microscopy (SEM) images showed alterations in the cell membrane of the treated parasites. The data obtained indicate a potential antiparasitic effect of the Piper species analyzed against P. serpens, being considered promising candidates for formulations of bioproducts to control the parasite. |
| first_indexed | 2024-12-20T16:19:55Z |
| format | Article |
| id | doaj.art-905ade58905f42f19ac508a1c32fadc7 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-12-20T16:19:55Z |
| publishDate | 2020-08-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-905ade58905f42f19ac508a1c32fadc72022-12-21T19:33:40ZengUniversidade Federal de Santa MariaCiência Rural1678-45962020-08-01501010.1590/0103-8478cr20200343Activity of Piperaceae extracts and fractions in the control of Phytomonas serpensNeriani de Souza Cancinihttps://orcid.org/0000-0002-2306-7545Jesieli Beraldo-Borrazzohttps://orcid.org/0000-0002-7628-3057Jéssica Lima de Menezeshttps://orcid.org/0000-0003-3036-3139Diógenes Aparício Garcia CortezRodrigo Hinojosa Valdezhttps://orcid.org/0000-0002-4420-8327Francielle Pelegrin Garciahttps://orcid.org/0000-0001-8966-9154Sueli Fumie Yamada-Ogattahttps://orcid.org/0000-0003-1183-146XBenedito Prado Dias Filhohttps://orcid.org/0000-0002-7300-397XTânia Ueda-Nakamurahttps://orcid.org/0000-0001-5057-3518Celso Vataru Nakamurahttps://orcid.org/0000-0002-9911-7369Benício Alves de Abreu Filhohttps://orcid.org/0000-0002-3271-808XABSTRACT: Protozoa of the genus Phytomonas are harmful parasites to several agricultural crops of economic importance. Due to their recognized biological activity, crude extracts of Piper aduncum, P. crassinervium, P. hispidum, and P. amalago leaves, were tested using the microdilution plate technique to assess the antiparasitic potential against Phytomonas serpens. Results showed that the ethanolic crude extract of P. crassinervium and P. amalago presented the best inhibitory concentration for 50% of the cells (IC50), 16.5 µg mL-1 in chloroform phase, and 18 µg mL-1 in aqueous phase, respectively, after 48 h treatment. Cytotoxicity analyses were performed using the colorimetric method of sulforhodamine-B in LLCMK2 mammalian cells. The chloroform phase of P. crassinervium was subjected to the fractionation process, in which the ethyl acetate and dichloromethane fractions obtained better IC50 values. Scanning electron microscopy (SEM) images showed alterations in the cell membrane of the treated parasites. The data obtained indicate a potential antiparasitic effect of the Piper species analyzed against P. serpens, being considered promising candidates for formulations of bioproducts to control the parasite.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001000152&tlng=enantiparasitic activitymedicinal plantsPiperTrypanosomatidPhytomonas |
| spellingShingle | Neriani de Souza Cancini Jesieli Beraldo-Borrazzo Jéssica Lima de Menezes Diógenes Aparício Garcia Cortez Rodrigo Hinojosa Valdez Francielle Pelegrin Garcia Sueli Fumie Yamada-Ogatta Benedito Prado Dias Filho Tânia Ueda-Nakamura Celso Vataru Nakamura Benício Alves de Abreu Filho Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens Ciência Rural antiparasitic activity medicinal plants Piper Trypanosomatid Phytomonas |
| title | Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens |
| title_full | Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens |
| title_fullStr | Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens |
| title_full_unstemmed | Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens |
| title_short | Activity of Piperaceae extracts and fractions in the control of Phytomonas serpens |
| title_sort | activity of piperaceae extracts and fractions in the control of phytomonas serpens |
| topic | antiparasitic activity medicinal plants Piper Trypanosomatid Phytomonas |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001000152&tlng=en |
| work_keys_str_mv | AT nerianidesouzacancini activityofpiperaceaeextractsandfractionsinthecontrolofphytomonasserpens AT jesieliberaldoborrazzo activityofpiperaceaeextractsandfractionsinthecontrolofphytomonasserpens AT jessicalimademenezes activityofpiperaceaeextractsandfractionsinthecontrolofphytomonasserpens AT diogenesapariciogarciacortez activityofpiperaceaeextractsandfractionsinthecontrolofphytomonasserpens AT rodrigohinojosavaldez activityofpiperaceaeextractsandfractionsinthecontrolofphytomonasserpens AT franciellepelegringarcia activityofpiperaceaeextractsandfractionsinthecontrolofphytomonasserpens AT suelifumieyamadaogatta activityofpiperaceaeextractsandfractionsinthecontrolofphytomonasserpens AT beneditopradodiasfilho activityofpiperaceaeextractsandfractionsinthecontrolofphytomonasserpens AT taniauedanakamura activityofpiperaceaeextractsandfractionsinthecontrolofphytomonasserpens AT celsovatarunakamura activityofpiperaceaeextractsandfractionsinthecontrolofphytomonasserpens AT benicioalvesdeabreufilho activityofpiperaceaeextractsandfractionsinthecontrolofphytomonasserpens |