Effect of pH and Hypo-Osmotic Stress on NaCl Electrolyte Permeation across Red Blood Cell Membrane
The study compares the effects produced by H+ concentrations in hypo-osmotically stressed NaCl solutions on permeation through RBC membrane. Iso-osmotic solutions of NaCl (160 mM) were prepared taking into account their molecular weight and osmotic concentration. Iso-osmotic solutions (25 mL) were...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2019-08-01
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| Series: | Journal of Applied Sciences and Environmental Management |
| Subjects: | |
| Online Access: | https://www.ajol.info/index.php/jasem/article/view/188797 |
| Summary: | The study compares the effects produced by H+ concentrations in hypo-osmotically stressed NaCl solutions on permeation through RBC membrane. Iso-osmotic solutions of NaCl (160 mM) were prepared taking into account their molecular weight and osmotic concentration. Iso-osmotic solutions (25 mL) were diluted with 25 mL of distilled water to give half the initial concentrations (50 % hypotonic salt solutions). Hypotonic NaCl solutions of pH of 6.2, 6.8, 7.4 and 8.0 were prepared, mixed with 0.05 mL of RBC suspension and their absorbance/min for 16 min were measured using a UV/Visible spectrophotometer while the RBCs haemolyse. H+ concentration and hence pH was found to have a significant effect on ion permeability through RBC membrane. Permeation occurred faster in alkalinic solutions under hypo-osmotic stress. Permeation increased with every increase in pH and the increase is steeper at pH 7.4 – 8.0.
Keywords: Ions, Electrolyte, Permeability, Red Blood Cell, Membrane
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| ISSN: | 2659-1502 2659-1499 |