625 nm Light Irradiation Prevented MC3T3-E1 Cells from Accumulation of Misfolded Proteins via ROS and ATP Production
Osteoblasts must acquire a considerable capacity for folding unfolded and misfolded proteins (MPs) to produce large amounts of extracellular matrix proteins and maintain bone homeostasis. MP accumulation contributes to cellular apoptosis and bone disorders. Photobiomodulation therapy has been used t...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/1422-0067/24/11/9257 |
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| author | Wenqi Fu Yeong-Gwan Im Byunggook Kim Ok-Su Kim Ying Yang Jianan Song Danyang Liu Siyu Zhu Jae-Seok Kang Okjoon Kim |
| author_facet | Wenqi Fu Yeong-Gwan Im Byunggook Kim Ok-Su Kim Ying Yang Jianan Song Danyang Liu Siyu Zhu Jae-Seok Kang Okjoon Kim |
| author_sort | Wenqi Fu |
| collection | DOAJ |
| description | Osteoblasts must acquire a considerable capacity for folding unfolded and misfolded proteins (MPs) to produce large amounts of extracellular matrix proteins and maintain bone homeostasis. MP accumulation contributes to cellular apoptosis and bone disorders. Photobiomodulation therapy has been used to treat bone diseases, but the effects of decreasing MPs with photobiomodulation remain unclear. In this study, we explored the efficacy of 625 nm light-emitting diode irradiation (LEDI) to reduce MPs in tunicamycin (TM) induced-MC3T3-E1 cells. Binding immunoglobulin protein (BiP), an adenosine triphosphate (ATP)-dependent chaperone, is used to evaluate the capacity of folding MPs. The results revealed that pretreatment with 625 nm LEDI (Pre-IR) induced reactive oxygen species (ROS) production, leading to the increased chaperone BiP through the inositol-requiring enzyme 1 (IRE1)/X-box binding protein 1s (XBP-1s) pathway, and then restoration of collagen type I (COL-I) and osteopontin (OPN) expression relieving cell apoptosis. Furthermore, the translocation of BiP into the endoplasmic reticulum (ER) lumen might be followed by a high level of ATP production. Taken together, these results suggest that Pre-IR could be beneficial to prevent MP accumulation through ROS and ATP in TM-induced MC3T3-E1cells. |
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| format | Article |
| id | doaj.art-905cda67f3bd4ad0a14e979c2b7352bb |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:05:47Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-905cda67f3bd4ad0a14e979c2b7352bb2023-11-18T07:56:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012411925710.3390/ijms24119257625 nm Light Irradiation Prevented MC3T3-E1 Cells from Accumulation of Misfolded Proteins via ROS and ATP ProductionWenqi Fu0Yeong-Gwan Im1Byunggook Kim2Ok-Su Kim3Ying Yang4Jianan Song5Danyang Liu6Siyu Zhu7Jae-Seok Kang8Okjoon Kim9Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Oral Medicine, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Oral Medicine, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Periodontology, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju 61186, Republic of KoreaOsteoblasts must acquire a considerable capacity for folding unfolded and misfolded proteins (MPs) to produce large amounts of extracellular matrix proteins and maintain bone homeostasis. MP accumulation contributes to cellular apoptosis and bone disorders. Photobiomodulation therapy has been used to treat bone diseases, but the effects of decreasing MPs with photobiomodulation remain unclear. In this study, we explored the efficacy of 625 nm light-emitting diode irradiation (LEDI) to reduce MPs in tunicamycin (TM) induced-MC3T3-E1 cells. Binding immunoglobulin protein (BiP), an adenosine triphosphate (ATP)-dependent chaperone, is used to evaluate the capacity of folding MPs. The results revealed that pretreatment with 625 nm LEDI (Pre-IR) induced reactive oxygen species (ROS) production, leading to the increased chaperone BiP through the inositol-requiring enzyme 1 (IRE1)/X-box binding protein 1s (XBP-1s) pathway, and then restoration of collagen type I (COL-I) and osteopontin (OPN) expression relieving cell apoptosis. Furthermore, the translocation of BiP into the endoplasmic reticulum (ER) lumen might be followed by a high level of ATP production. Taken together, these results suggest that Pre-IR could be beneficial to prevent MP accumulation through ROS and ATP in TM-induced MC3T3-E1cells.https://www.mdpi.com/1422-0067/24/11/9257light-emitting diode irradiation (LEDI)binding immunoglobulin protein (BiP)reactive oxygen species (ROS)adenosine triphosphate (ATP)endoplasmic reticulum (ER) stressbone |
| spellingShingle | Wenqi Fu Yeong-Gwan Im Byunggook Kim Ok-Su Kim Ying Yang Jianan Song Danyang Liu Siyu Zhu Jae-Seok Kang Okjoon Kim 625 nm Light Irradiation Prevented MC3T3-E1 Cells from Accumulation of Misfolded Proteins via ROS and ATP Production International Journal of Molecular Sciences light-emitting diode irradiation (LEDI) binding immunoglobulin protein (BiP) reactive oxygen species (ROS) adenosine triphosphate (ATP) endoplasmic reticulum (ER) stress bone |
| title | 625 nm Light Irradiation Prevented MC3T3-E1 Cells from Accumulation of Misfolded Proteins via ROS and ATP Production |
| title_full | 625 nm Light Irradiation Prevented MC3T3-E1 Cells from Accumulation of Misfolded Proteins via ROS and ATP Production |
| title_fullStr | 625 nm Light Irradiation Prevented MC3T3-E1 Cells from Accumulation of Misfolded Proteins via ROS and ATP Production |
| title_full_unstemmed | 625 nm Light Irradiation Prevented MC3T3-E1 Cells from Accumulation of Misfolded Proteins via ROS and ATP Production |
| title_short | 625 nm Light Irradiation Prevented MC3T3-E1 Cells from Accumulation of Misfolded Proteins via ROS and ATP Production |
| title_sort | 625 nm light irradiation prevented mc3t3 e1 cells from accumulation of misfolded proteins via ros and atp production |
| topic | light-emitting diode irradiation (LEDI) binding immunoglobulin protein (BiP) reactive oxygen species (ROS) adenosine triphosphate (ATP) endoplasmic reticulum (ER) stress bone |
| url | https://www.mdpi.com/1422-0067/24/11/9257 |
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