Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients
Macrolide-resistant methicillin-resistant <i>Staphylococcus aureus</i> (MAC-MRSA) is one of the most clinically relevant pathogens due to its significant ability of resistance acquisition to different antimicrobial agents. This study aimed to evaluate antimicrobial susceptibility and the...
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MDPI AG
2021-07-01
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| author | Amr S. Bishr Salma M. Abdelaziz Ibrahim S. Yahia Mahmoud A. Yassien Nadia A. Hassouna Khaled M. Aboshanab |
| author_facet | Amr S. Bishr Salma M. Abdelaziz Ibrahim S. Yahia Mahmoud A. Yassien Nadia A. Hassouna Khaled M. Aboshanab |
| author_sort | Amr S. Bishr |
| collection | DOAJ |
| description | Macrolide-resistant methicillin-resistant <i>Staphylococcus aureus</i> (MAC-MRSA) is one of the most clinically relevant pathogens due to its significant ability of resistance acquisition to different antimicrobial agents. This study aimed to evaluate antimicrobial susceptibility and the use of different combinations of azithromycin with other antibiotics for combating MAC resistance. Seventy-two <i>Staphylococci</i> (38.5%) (n = 187), showed resistance to MACs; of these, 53 isolates (73.6%, n = 72) were <i>S. aureus</i> and 19 (26.4%, n = 72) were coagulase-negative staphylococci (CoNS). Out of the 53 <i>S. aureus</i> and 19 CoNS isolates, 38 (71.7%, n = 53) and 9 (47.4%, n = 19) were MRSA and methicillin-resistant CoNS, respectively. The constitutive MACs, lincosamides and streptogramin-B (cMLS) comprised the predominant phenotype among <i>S. aureus</i> isolates (54.7%) and CoNS isolates (78.9%). The PCR analysis showed that the <i>erm</i>C gene was the most prevalent (79.2%), followed by <i>msr</i>A (48.6%), and <i>erm</i>A (31.9%). Azithromycin combinations with either linezolid, ceftriaxone, gentamicin, or cefotaxime provided synergy in 42.1%, 44.7%, 31.6% and 7.9% of the 38 MAC-MRSA isolates, respectively. Statistical analysis showed significant association between certain MAC resistance genotypes and the synergistic effect of certain azithromycin combinations (<i>p</i> value < 0.05). In conclusion, azithromycin combinations with either linezolid, or ceftriaxone showed synergism in most of the MAC-resistant MRSA clinical isolates. |
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| language | English |
| last_indexed | 2024-03-10T09:45:10Z |
| publishDate | 2021-07-01 |
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| spelling | doaj.art-905f1ae8201441f6a9e363236a99eaf92023-11-22T03:15:31ZengMDPI AGBiology2079-77372021-07-0110762410.3390/biology10070624Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized PatientsAmr S. Bishr0Salma M. Abdelaziz1Ibrahim S. Yahia2Mahmoud A. Yassien3Nadia A. Hassouna4Khaled M. Aboshanab5Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., Cairo 11566, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., Cairo 11566, EgyptResearch Center for Advanced Materials Science (RCAMS), King Khalid University, Abha P.O. Box 9004, Saudi ArabiaDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., Cairo 11566, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., Cairo 11566, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., Cairo 11566, EgyptMacrolide-resistant methicillin-resistant <i>Staphylococcus aureus</i> (MAC-MRSA) is one of the most clinically relevant pathogens due to its significant ability of resistance acquisition to different antimicrobial agents. This study aimed to evaluate antimicrobial susceptibility and the use of different combinations of azithromycin with other antibiotics for combating MAC resistance. Seventy-two <i>Staphylococci</i> (38.5%) (n = 187), showed resistance to MACs; of these, 53 isolates (73.6%, n = 72) were <i>S. aureus</i> and 19 (26.4%, n = 72) were coagulase-negative staphylococci (CoNS). Out of the 53 <i>S. aureus</i> and 19 CoNS isolates, 38 (71.7%, n = 53) and 9 (47.4%, n = 19) were MRSA and methicillin-resistant CoNS, respectively. The constitutive MACs, lincosamides and streptogramin-B (cMLS) comprised the predominant phenotype among <i>S. aureus</i> isolates (54.7%) and CoNS isolates (78.9%). The PCR analysis showed that the <i>erm</i>C gene was the most prevalent (79.2%), followed by <i>msr</i>A (48.6%), and <i>erm</i>A (31.9%). Azithromycin combinations with either linezolid, ceftriaxone, gentamicin, or cefotaxime provided synergy in 42.1%, 44.7%, 31.6% and 7.9% of the 38 MAC-MRSA isolates, respectively. Statistical analysis showed significant association between certain MAC resistance genotypes and the synergistic effect of certain azithromycin combinations (<i>p</i> value < 0.05). In conclusion, azithromycin combinations with either linezolid, or ceftriaxone showed synergism in most of the MAC-resistant MRSA clinical isolates.https://www.mdpi.com/2079-7737/10/7/624macrolide resistancemethicillin-resistant <i>Staphylococcus aureus</i>coagulase-negative staphylococcicMLSiMLSMS |
| spellingShingle | Amr S. Bishr Salma M. Abdelaziz Ibrahim S. Yahia Mahmoud A. Yassien Nadia A. Hassouna Khaled M. Aboshanab Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients Biology macrolide resistance methicillin-resistant <i>Staphylococcus aureus</i> coagulase-negative staphylococci cMLS iMLS MS |
| title | Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients |
| title_full | Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients |
| title_fullStr | Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients |
| title_full_unstemmed | Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients |
| title_short | Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients |
| title_sort | association of macrolide resistance genotypes and synergistic antibiotic combinations for combating macrolide resistant mrsa recovered from hospitalized patients |
| topic | macrolide resistance methicillin-resistant <i>Staphylococcus aureus</i> coagulase-negative staphylococci cMLS iMLS MS |
| url | https://www.mdpi.com/2079-7737/10/7/624 |
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