Comparative immunohistochemical study of acquired cholesteatoma in children and adults
Abstract Objective To compare the histopathological structure and immunohistochemical characteristics of acquired cholesteatoma in children and adults. Patients and Methods This study was carried out on 40 patients presenting with cholesteatomatous middle-ear disease. Twenty patients were of a pedia...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-05-01
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| Series: | The Egyptian Journal of Otolaryngology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.4103/ejo.ejo_83_17 |
| _version_ | 1797204060792684544 |
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| author | Mohammed Bassiouny Nahed Badour Yasser Shewel |
| author_facet | Mohammed Bassiouny Nahed Badour Yasser Shewel |
| author_sort | Mohammed Bassiouny |
| collection | DOAJ |
| description | Abstract Objective To compare the histopathological structure and immunohistochemical characteristics of acquired cholesteatoma in children and adults. Patients and Methods This study was carried out on 40 patients presenting with cholesteatomatous middle-ear disease. Twenty patients were of a pediatric age group (<18 years) and the rest were adults (>18 years). Patients were admitted to the ENT Department of Alexandria University Hospital. All cholesteatoma specimens were collected intraoperatively and preserved for histopathological examination and immunohistochemical technique using the epidermal growth factor (EGF) monoclonal antibody. Results Histopathological examination of the submitted specimens showed that strips of stratified squamous epithelium with the underlying tissues were fibrous in adults, whereas cellular inflammatory infiltrates were observed in children. The degree of fibrosis was significantly higher in the adult group, whereas the pediatric group had higher inflammatory infiltrate. Immunohistochemical examination indicated significantly higher expression of EGF in children compared with adults both in the matrix and in the perimatrix of acquired cholesteatoma. Conclusion Children with acquired cholesteatoma had higher inflammatory infiltration and significant expression of EGF in both the matrix and the perimatrix with less fibrosis compared with adults, explaining the possible pathogenesis of aggressive behavior of cholesteatoma in children. |
| first_indexed | 2024-04-24T08:29:14Z |
| format | Article |
| id | doaj.art-905f6e6d08204db9882f7fa52bd569f7 |
| institution | Directory Open Access Journal |
| issn | 1012-5574 2090-8539 |
| language | English |
| last_indexed | 2024-04-24T08:29:14Z |
| publishDate | 2018-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | The Egyptian Journal of Otolaryngology |
| spelling | doaj.art-905f6e6d08204db9882f7fa52bd569f72024-04-16T20:50:58ZengSpringerOpenThe Egyptian Journal of Otolaryngology1012-55742090-85392018-05-0134211612110.4103/ejo.ejo_83_17Comparative immunohistochemical study of acquired cholesteatoma in children and adultsMohammed Bassiouny0Nahed Badour1Yasser Shewel2Department of Otolaryngology, Alexandria School of Medicine, University of AlexandriaDepartment of Histology, Alexandria School of Medicine, University of AlexandriaDepartment of Otolaryngology, Alexandria School of Medicine, University of AlexandriaAbstract Objective To compare the histopathological structure and immunohistochemical characteristics of acquired cholesteatoma in children and adults. Patients and Methods This study was carried out on 40 patients presenting with cholesteatomatous middle-ear disease. Twenty patients were of a pediatric age group (<18 years) and the rest were adults (>18 years). Patients were admitted to the ENT Department of Alexandria University Hospital. All cholesteatoma specimens were collected intraoperatively and preserved for histopathological examination and immunohistochemical technique using the epidermal growth factor (EGF) monoclonal antibody. Results Histopathological examination of the submitted specimens showed that strips of stratified squamous epithelium with the underlying tissues were fibrous in adults, whereas cellular inflammatory infiltrates were observed in children. The degree of fibrosis was significantly higher in the adult group, whereas the pediatric group had higher inflammatory infiltrate. Immunohistochemical examination indicated significantly higher expression of EGF in children compared with adults both in the matrix and in the perimatrix of acquired cholesteatoma. Conclusion Children with acquired cholesteatoma had higher inflammatory infiltration and significant expression of EGF in both the matrix and the perimatrix with less fibrosis compared with adults, explaining the possible pathogenesis of aggressive behavior of cholesteatoma in children.http://link.springer.com/article/10.4103/ejo.ejo_83_17cholesteatomaepidermal growth factorimmunohistochemistry |
| spellingShingle | Mohammed Bassiouny Nahed Badour Yasser Shewel Comparative immunohistochemical study of acquired cholesteatoma in children and adults The Egyptian Journal of Otolaryngology cholesteatoma epidermal growth factor immunohistochemistry |
| title | Comparative immunohistochemical study of acquired cholesteatoma in children and adults |
| title_full | Comparative immunohistochemical study of acquired cholesteatoma in children and adults |
| title_fullStr | Comparative immunohistochemical study of acquired cholesteatoma in children and adults |
| title_full_unstemmed | Comparative immunohistochemical study of acquired cholesteatoma in children and adults |
| title_short | Comparative immunohistochemical study of acquired cholesteatoma in children and adults |
| title_sort | comparative immunohistochemical study of acquired cholesteatoma in children and adults |
| topic | cholesteatoma epidermal growth factor immunohistochemistry |
| url | http://link.springer.com/article/10.4103/ejo.ejo_83_17 |
| work_keys_str_mv | AT mohammedbassiouny comparativeimmunohistochemicalstudyofacquiredcholesteatomainchildrenandadults AT nahedbadour comparativeimmunohistochemicalstudyofacquiredcholesteatomainchildrenandadults AT yassershewel comparativeimmunohistochemicalstudyofacquiredcholesteatomainchildrenandadults |