Assessing the relationship between gut microbiota and irritable bowel syndrome: a two-sample Mendelian randomization analysis
Abstract Background Growing evidence has suggested that gut microbiota is closely related to the risk of irritable bowel syndrome (IBS), but whether there is a causal effect remains unknown. We adopted a Mendelian randomization (MR) approach to evaluate the potential causal relationships between gut...
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| Format: | Article |
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BMC
2023-05-01
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| Series: | BMC Gastroenterology |
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| Online Access: | https://doi.org/10.1186/s12876-023-02791-7 |
| _version_ | 1797827518823137280 |
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| author | Bin Liu Ding Ye Hong Yang Jie Song Xiaohui Sun Zhixing He Yingying Mao Guifeng Hao |
| author_facet | Bin Liu Ding Ye Hong Yang Jie Song Xiaohui Sun Zhixing He Yingying Mao Guifeng Hao |
| author_sort | Bin Liu |
| collection | DOAJ |
| description | Abstract Background Growing evidence has suggested that gut microbiota is closely related to the risk of irritable bowel syndrome (IBS), but whether there is a causal effect remains unknown. We adopted a Mendelian randomization (MR) approach to evaluate the potential causal relationships between gut microbiota and the risk of IBS. Methods Genetic instrumental variables for gut microbiota were identified from a genome-wide association study (GWAS) of 18,340 participants. Summary statistics of IBS were drawn from a GWAS including 53,400 cases and 433,201 controls. We used the inverse-variance weighted (IVW) method as the primary analysis. To test the robustness of our results, we further performed the weighted-median method, MR-Egger regression, and MR pleiotropy residual sum and outlier test. Finally, reverse MR analysis was performed to evaluate the possibility of reverse causation. Results We identified suggestive associations between three bacterial traits and the risk of IBS (odds ratio (OR): 1.08; 95% confidence interval (CI): 1.02, 1.15; p = 0.011 for phylum Actinobacteria; OR: 0.95; 95% CI: 0.91, 1.00; p = 0.030 for genus Eisenbergiella and OR: 1.10; 95% CI: 1.03, 1.18; p = 0.005 for genus Flavonifractor). The results of sensitivity analyses for these bacterial traits were consistent. We did not find statistically significant associations between IBS and these three bacterial traits in the reverse MR analysis. Conclusions Our systematic analyses provide evidence to support a potential causal relationship between several gut microbiota taxa and the risk of IBS. More studies are required to show how the gut microbiota affects the development of IBS. |
| first_indexed | 2024-04-09T12:49:36Z |
| format | Article |
| id | doaj.art-906115688ea447109e65241f6bac9703 |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-04-09T12:49:36Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-906115688ea447109e65241f6bac97032023-05-14T11:18:42ZengBMCBMC Gastroenterology1471-230X2023-05-012311910.1186/s12876-023-02791-7Assessing the relationship between gut microbiota and irritable bowel syndrome: a two-sample Mendelian randomization analysisBin Liu0Ding Ye1Hong Yang2Jie Song3Xiaohui Sun4Zhixing He5Yingying Mao6Guifeng Hao7Department of Epidemiology, School of Public Health, Zhejiang Chinese Medical UniversityDepartment of Epidemiology, School of Public Health, Zhejiang Chinese Medical UniversityDepartment of Epidemiology, School of Public Health, Zhejiang Chinese Medical UniversityDepartment of Epidemiology, School of Public Health, Zhejiang Chinese Medical UniversityDepartment of Epidemiology, School of Public Health, Zhejiang Chinese Medical UniversityInstitute of Basic Research in Clinical Medicine, School of Basic Medical Science, Zhejiang Chinese Medical UniversityDepartment of Epidemiology, School of Public Health, Zhejiang Chinese Medical UniversityCenter for General Practice Medicine, Department of Rheumatology and Immunology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College)Abstract Background Growing evidence has suggested that gut microbiota is closely related to the risk of irritable bowel syndrome (IBS), but whether there is a causal effect remains unknown. We adopted a Mendelian randomization (MR) approach to evaluate the potential causal relationships between gut microbiota and the risk of IBS. Methods Genetic instrumental variables for gut microbiota were identified from a genome-wide association study (GWAS) of 18,340 participants. Summary statistics of IBS were drawn from a GWAS including 53,400 cases and 433,201 controls. We used the inverse-variance weighted (IVW) method as the primary analysis. To test the robustness of our results, we further performed the weighted-median method, MR-Egger regression, and MR pleiotropy residual sum and outlier test. Finally, reverse MR analysis was performed to evaluate the possibility of reverse causation. Results We identified suggestive associations between three bacterial traits and the risk of IBS (odds ratio (OR): 1.08; 95% confidence interval (CI): 1.02, 1.15; p = 0.011 for phylum Actinobacteria; OR: 0.95; 95% CI: 0.91, 1.00; p = 0.030 for genus Eisenbergiella and OR: 1.10; 95% CI: 1.03, 1.18; p = 0.005 for genus Flavonifractor). The results of sensitivity analyses for these bacterial traits were consistent. We did not find statistically significant associations between IBS and these three bacterial traits in the reverse MR analysis. Conclusions Our systematic analyses provide evidence to support a potential causal relationship between several gut microbiota taxa and the risk of IBS. More studies are required to show how the gut microbiota affects the development of IBS.https://doi.org/10.1186/s12876-023-02791-7Irritable bowel syndromeGut microbiotaMendelian randomizationSingle nucleotide polymorphism |
| spellingShingle | Bin Liu Ding Ye Hong Yang Jie Song Xiaohui Sun Zhixing He Yingying Mao Guifeng Hao Assessing the relationship between gut microbiota and irritable bowel syndrome: a two-sample Mendelian randomization analysis BMC Gastroenterology Irritable bowel syndrome Gut microbiota Mendelian randomization Single nucleotide polymorphism |
| title | Assessing the relationship between gut microbiota and irritable bowel syndrome: a two-sample Mendelian randomization analysis |
| title_full | Assessing the relationship between gut microbiota and irritable bowel syndrome: a two-sample Mendelian randomization analysis |
| title_fullStr | Assessing the relationship between gut microbiota and irritable bowel syndrome: a two-sample Mendelian randomization analysis |
| title_full_unstemmed | Assessing the relationship between gut microbiota and irritable bowel syndrome: a two-sample Mendelian randomization analysis |
| title_short | Assessing the relationship between gut microbiota and irritable bowel syndrome: a two-sample Mendelian randomization analysis |
| title_sort | assessing the relationship between gut microbiota and irritable bowel syndrome a two sample mendelian randomization analysis |
| topic | Irritable bowel syndrome Gut microbiota Mendelian randomization Single nucleotide polymorphism |
| url | https://doi.org/10.1186/s12876-023-02791-7 |
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