Weapons against Themselves: Identification and Use of Quorum Sensing Volatile Molecules to Control Plant Pathogenic Fungi Growth
Quorum sensing (QS) is often defined as a mechanism of microbial communication that can regulate microbial behaviors in accordance with population density. Much is known about QS mechanisms in bacteria, but fungal QS research is still in its infancy. In this study, the molecules constituting the vol...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/12/2459 |
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| author | Caroline De Clerck Laurie Josselin Valentine Vangoethem Ludivine Lassois Marie-Laure Fauconnier Haïssam Jijakli |
| author_facet | Caroline De Clerck Laurie Josselin Valentine Vangoethem Ludivine Lassois Marie-Laure Fauconnier Haïssam Jijakli |
| author_sort | Caroline De Clerck |
| collection | DOAJ |
| description | Quorum sensing (QS) is often defined as a mechanism of microbial communication that can regulate microbial behaviors in accordance with population density. Much is known about QS mechanisms in bacteria, but fungal QS research is still in its infancy. In this study, the molecules constituting the volatolomes of the plant pathogenic fungi <i>Fusarium culmorum</i> and <i>Cochliobolus sativus</i> have been identified during culture conditions involving low and high spore concentrations, with the high concentration imitating overpopulation conditions (for QS stimulation). We determined that volatolomes emitted by these species in conditions of overpopulation have a negative impact on their mycelial growth, with some of the emitted molecules possibly acting as QSM. Candidate VOCs related to QS have then been identified by testing the effect of individual volatile organic compounds (VOCs) on mycelial growth of their emitting species. The antifungal effect observed for the volatolome of <i>F. culmorum</i> in the overpopulation condition could be attributed to ethyl acetate, 2-methylpropan-1-ol, 3-methylbutyl ethanoate, 3-methylbutan-1-ol, and pentan-1-ol, while it could be attributed to longifolene, 3-methylbutan-1-ol, 2-methylpropan-1-ol, and ethyl acetate for <i>C. sativus</i> in the overpopulation condition. This work could pave the way to a sustainable alternative to chemical fungicides. |
| first_indexed | 2024-03-09T16:04:23Z |
| format | Article |
| id | doaj.art-90611b9ce02444f793dbe14504875fa9 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:04:23Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-90611b9ce02444f793dbe14504875fa92023-11-24T16:49:27ZengMDPI AGMicroorganisms2076-26072022-12-011012245910.3390/microorganisms10122459Weapons against Themselves: Identification and Use of Quorum Sensing Volatile Molecules to Control Plant Pathogenic Fungi GrowthCaroline De Clerck0Laurie Josselin1Valentine Vangoethem2Ludivine Lassois3Marie-Laure Fauconnier4Haïssam Jijakli5AgricultureIsLife, Gembloux Agro-Bio Tech, Liege University, Passage des Déportés 2, 5030 Gembloux, BelgiumLaboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, Liege University, Passage des Déportés 2, 5030 Gembloux, BelgiumIntegrated and Urban Plant Pathology Laboratory, Gembloux Agro-Bio Tech, Liege University, Passage des Déportés 2, 5030 Gembloux, BelgiumPlant Genetics and Rhizosphere Processes Lab., Gembloux Agro-Bio Tech, Liege University, Passage des Déportés 2, 5030 Gembloux, BelgiumLaboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, Liege University, Passage des Déportés 2, 5030 Gembloux, BelgiumIntegrated and Urban Plant Pathology Laboratory, Gembloux Agro-Bio Tech, Liege University, Passage des Déportés 2, 5030 Gembloux, BelgiumQuorum sensing (QS) is often defined as a mechanism of microbial communication that can regulate microbial behaviors in accordance with population density. Much is known about QS mechanisms in bacteria, but fungal QS research is still in its infancy. In this study, the molecules constituting the volatolomes of the plant pathogenic fungi <i>Fusarium culmorum</i> and <i>Cochliobolus sativus</i> have been identified during culture conditions involving low and high spore concentrations, with the high concentration imitating overpopulation conditions (for QS stimulation). We determined that volatolomes emitted by these species in conditions of overpopulation have a negative impact on their mycelial growth, with some of the emitted molecules possibly acting as QSM. Candidate VOCs related to QS have then been identified by testing the effect of individual volatile organic compounds (VOCs) on mycelial growth of their emitting species. The antifungal effect observed for the volatolome of <i>F. culmorum</i> in the overpopulation condition could be attributed to ethyl acetate, 2-methylpropan-1-ol, 3-methylbutyl ethanoate, 3-methylbutan-1-ol, and pentan-1-ol, while it could be attributed to longifolene, 3-methylbutan-1-ol, 2-methylpropan-1-ol, and ethyl acetate for <i>C. sativus</i> in the overpopulation condition. This work could pave the way to a sustainable alternative to chemical fungicides.https://www.mdpi.com/2076-2607/10/12/2459quorum sensing<i>Fusarium culmorum</i><i>Cochliobolus sativus</i>volatile organic compounds (VOCs)antifungal |
| spellingShingle | Caroline De Clerck Laurie Josselin Valentine Vangoethem Ludivine Lassois Marie-Laure Fauconnier Haïssam Jijakli Weapons against Themselves: Identification and Use of Quorum Sensing Volatile Molecules to Control Plant Pathogenic Fungi Growth Microorganisms quorum sensing <i>Fusarium culmorum</i> <i>Cochliobolus sativus</i> volatile organic compounds (VOCs) antifungal |
| title | Weapons against Themselves: Identification and Use of Quorum Sensing Volatile Molecules to Control Plant Pathogenic Fungi Growth |
| title_full | Weapons against Themselves: Identification and Use of Quorum Sensing Volatile Molecules to Control Plant Pathogenic Fungi Growth |
| title_fullStr | Weapons against Themselves: Identification and Use of Quorum Sensing Volatile Molecules to Control Plant Pathogenic Fungi Growth |
| title_full_unstemmed | Weapons against Themselves: Identification and Use of Quorum Sensing Volatile Molecules to Control Plant Pathogenic Fungi Growth |
| title_short | Weapons against Themselves: Identification and Use of Quorum Sensing Volatile Molecules to Control Plant Pathogenic Fungi Growth |
| title_sort | weapons against themselves identification and use of quorum sensing volatile molecules to control plant pathogenic fungi growth |
| topic | quorum sensing <i>Fusarium culmorum</i> <i>Cochliobolus sativus</i> volatile organic compounds (VOCs) antifungal |
| url | https://www.mdpi.com/2076-2607/10/12/2459 |
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