Unraveling the Role of Cytochrome P450 as a Key Regulator Lantipeptide Production in Streptomyces globisporus
The aim of this study was to investigate the regulation of lantipeptide production in Streptomyces globisporus SP6C4, which produces the novel antifungal lantipeptides conprimycin and grisin, and to identify the role of cytochrome P450 (P450) in tis regulation. To investigate the regulation of lanti...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2023-12-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://ppjonline.org/upload/pdf/PPJ-OA-08-2023-0119.pdf |
| _version_ | 1797394900148289536 |
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| author | Da-Ran Kim Su In Lee Youn-Sig Kwak |
| author_facet | Da-Ran Kim Su In Lee Youn-Sig Kwak |
| author_sort | Da-Ran Kim |
| collection | DOAJ |
| description | The aim of this study was to investigate the regulation of lantipeptide production in Streptomyces globisporus SP6C4, which produces the novel antifungal lantipeptides conprimycin and grisin, and to identify the role of cytochrome P450 (P450) in tis regulation. To investigate the regulation of lantipeptide production, we created gene deletion mutants, including ΔP450, ΔtsrD, ΔlanM, ΔP450ΔtsrD, and ΔP450ΔlanM. These mutants were characterized in terms of their morphology, sporulation, attachment, and antifungal activity against Fusarium oxysporum. The gene deletion mutants showed distinct characteristics compared to the wild-type strain. Among them, the ΔP450ΔlanM double mutant exhibited a recovery of antifungal activity against F. oxysporum, indicating that P450 plays a significant role in regulating lantipeptide production in S. globisporus SP6C4. Our findings highlight the significant role of P450 in the regulation of lantipeptide production and morphological processes in S. globisporus. The results suggest a potential link between P450-mediated metabolic pathways and the regulation of growth and secondary metabolism in SP6C4, thereby highlighting P450 as a putative target for the development of new antifungal agents. |
| first_indexed | 2024-03-09T00:26:28Z |
| format | Article |
| id | doaj.art-906135f9172748ea8c7c487d8e1813b2 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-03-09T00:26:28Z |
| publishDate | 2023-12-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-906135f9172748ea8c7c487d8e1813b22023-12-12T00:28:14ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802023-12-0139656657410.5423/PPJ.OA.08.2023.01192416Unraveling the Role of Cytochrome P450 as a Key Regulator Lantipeptide Production in Streptomyces globisporusDa-Ran Kim0Su In Lee1Youn-Sig Kwak2 Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Korea Division of Applied Life Science (BK21Plus), Gyeongsang National University, Jinju 52828, Korea Research Institute of Life Science, Gyeongsang National University, Jinju 52828, KoreaThe aim of this study was to investigate the regulation of lantipeptide production in Streptomyces globisporus SP6C4, which produces the novel antifungal lantipeptides conprimycin and grisin, and to identify the role of cytochrome P450 (P450) in tis regulation. To investigate the regulation of lantipeptide production, we created gene deletion mutants, including ΔP450, ΔtsrD, ΔlanM, ΔP450ΔtsrD, and ΔP450ΔlanM. These mutants were characterized in terms of their morphology, sporulation, attachment, and antifungal activity against Fusarium oxysporum. The gene deletion mutants showed distinct characteristics compared to the wild-type strain. Among them, the ΔP450ΔlanM double mutant exhibited a recovery of antifungal activity against F. oxysporum, indicating that P450 plays a significant role in regulating lantipeptide production in S. globisporus SP6C4. Our findings highlight the significant role of P450 in the regulation of lantipeptide production and morphological processes in S. globisporus. The results suggest a potential link between P450-mediated metabolic pathways and the regulation of growth and secondary metabolism in SP6C4, thereby highlighting P450 as a putative target for the development of new antifungal agents.http://ppjonline.org/upload/pdf/PPJ-OA-08-2023-0119.pdfbiological controlcytochrome p450fusarium oxysporumstreptomyces |
| spellingShingle | Da-Ran Kim Su In Lee Youn-Sig Kwak Unraveling the Role of Cytochrome P450 as a Key Regulator Lantipeptide Production in Streptomyces globisporus The Plant Pathology Journal biological control cytochrome p450 fusarium oxysporum streptomyces |
| title | Unraveling the Role of Cytochrome P450 as a Key Regulator Lantipeptide Production in Streptomyces globisporus |
| title_full | Unraveling the Role of Cytochrome P450 as a Key Regulator Lantipeptide Production in Streptomyces globisporus |
| title_fullStr | Unraveling the Role of Cytochrome P450 as a Key Regulator Lantipeptide Production in Streptomyces globisporus |
| title_full_unstemmed | Unraveling the Role of Cytochrome P450 as a Key Regulator Lantipeptide Production in Streptomyces globisporus |
| title_short | Unraveling the Role of Cytochrome P450 as a Key Regulator Lantipeptide Production in Streptomyces globisporus |
| title_sort | unraveling the role of cytochrome p450 as a key regulator lantipeptide production in streptomyces globisporus |
| topic | biological control cytochrome p450 fusarium oxysporum streptomyces |
| url | http://ppjonline.org/upload/pdf/PPJ-OA-08-2023-0119.pdf |
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