Evaluation of the antibacterial synergism of two plant extracts belonging to Bignoniaceae family and development of a topical formulation
Abstract Fridericia caudigera and Cuspidaria convoluta (Bignoniaceae) species, which grow in the northwest of Argentina, have shown antibacterial effect against strains isolated from skin infections, and each one displayed synergism with commercial antibiotics. The aims of this work were to evaluate...
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Format: | Article |
Language: | English |
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Universidade de São Paulo
2023-01-01
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Series: | Brazilian Journal of Pharmaceutical Sciences |
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100403&tlng=en |
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author | Carola Analía Torres Cristina Marisel Pérez Zamora María Beatriz Nuñez María Inés Isla Ana María Gonzalez Iris Catiana Zampini |
author_facet | Carola Analía Torres Cristina Marisel Pérez Zamora María Beatriz Nuñez María Inés Isla Ana María Gonzalez Iris Catiana Zampini |
author_sort | Carola Analía Torres |
collection | DOAJ |
description | Abstract Fridericia caudigera and Cuspidaria convoluta (Bignoniaceae) species, which grow in the northwest of Argentina, have shown antibacterial effect against strains isolated from skin infections, and each one displayed synergism with commercial antibiotics. The aims of this work were to evaluate the antibacterial activity and toxicity of the combination of these two plant species, and to design a stable gel for topical use including the blend of extracts. The combination of extracts was evaluated for synergistic effects (chequerboard assay), genotoxicity (Ames test) and cytotoxicity (Artemia salina test). A gel was subsequently formulated with the combination of extracts using carboxymethylcellulose as a polymer. The following physico- chemical characteristics of the gel formulation: pH, viscosity, spreadability and total phenol content, as well as resistance to severe temperature changes, biological activity (diffusion in agar), in vitro permeation (Franz cells) and primary dermal irritation (Draize test) were analyzed. The combination of extracts showed a synergistic effect on pathogenic bacteria and was not toxic in the in vitro tests. The gel was stable and retained the antimicrobial activity of the original extracts. The formulation proposed in this work could constitute an alternative for primary skin infections since it proved to be safe for topical administration. |
first_indexed | 2024-04-10T22:29:50Z |
format | Article |
id | doaj.art-9063628fe508420aba95146168cbf5d6 |
institution | Directory Open Access Journal |
issn | 2175-9790 |
language | English |
last_indexed | 2024-04-10T22:29:50Z |
publishDate | 2023-01-01 |
publisher | Universidade de São Paulo |
record_format | Article |
series | Brazilian Journal of Pharmaceutical Sciences |
spelling | doaj.art-9063628fe508420aba95146168cbf5d62023-01-17T07:34:03ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902023-01-015810.1590/s2175-97902022e201130Evaluation of the antibacterial synergism of two plant extracts belonging to Bignoniaceae family and development of a topical formulationCarola Analía Torreshttps://orcid.org/0000-0003-1772-7786Cristina Marisel Pérez ZamoraMaría Beatriz NuñezMaría Inés IslaAna María GonzalezIris Catiana ZampiniAbstract Fridericia caudigera and Cuspidaria convoluta (Bignoniaceae) species, which grow in the northwest of Argentina, have shown antibacterial effect against strains isolated from skin infections, and each one displayed synergism with commercial antibiotics. The aims of this work were to evaluate the antibacterial activity and toxicity of the combination of these two plant species, and to design a stable gel for topical use including the blend of extracts. The combination of extracts was evaluated for synergistic effects (chequerboard assay), genotoxicity (Ames test) and cytotoxicity (Artemia salina test). A gel was subsequently formulated with the combination of extracts using carboxymethylcellulose as a polymer. The following physico- chemical characteristics of the gel formulation: pH, viscosity, spreadability and total phenol content, as well as resistance to severe temperature changes, biological activity (diffusion in agar), in vitro permeation (Franz cells) and primary dermal irritation (Draize test) were analyzed. The combination of extracts showed a synergistic effect on pathogenic bacteria and was not toxic in the in vitro tests. The gel was stable and retained the antimicrobial activity of the original extracts. The formulation proposed in this work could constitute an alternative for primary skin infections since it proved to be safe for topical administration.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100403&tlng=enFridericia caudigeraCuspidaria convolutaGenotoxicityFranz cellsTopical gel |
spellingShingle | Carola Analía Torres Cristina Marisel Pérez Zamora María Beatriz Nuñez María Inés Isla Ana María Gonzalez Iris Catiana Zampini Evaluation of the antibacterial synergism of two plant extracts belonging to Bignoniaceae family and development of a topical formulation Brazilian Journal of Pharmaceutical Sciences Fridericia caudigera Cuspidaria convoluta Genotoxicity Franz cells Topical gel |
title | Evaluation of the antibacterial synergism of two plant extracts belonging to Bignoniaceae family and development of a topical formulation |
title_full | Evaluation of the antibacterial synergism of two plant extracts belonging to Bignoniaceae family and development of a topical formulation |
title_fullStr | Evaluation of the antibacterial synergism of two plant extracts belonging to Bignoniaceae family and development of a topical formulation |
title_full_unstemmed | Evaluation of the antibacterial synergism of two plant extracts belonging to Bignoniaceae family and development of a topical formulation |
title_short | Evaluation of the antibacterial synergism of two plant extracts belonging to Bignoniaceae family and development of a topical formulation |
title_sort | evaluation of the antibacterial synergism of two plant extracts belonging to bignoniaceae family and development of a topical formulation |
topic | Fridericia caudigera Cuspidaria convoluta Genotoxicity Franz cells Topical gel |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100403&tlng=en |
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