Anti-cancer effects of DHP107 on canine mammary gland cancer examined through in-vitro and in-vivo mouse xenograft models
Abstract Background Canine mammary gland cancer (CMGC) is a common neoplasm in intact bitches. However, the benefit of adjuvant chemotherapy is unclear. The aim of this study was to investigate the anti-proliferative effects of paclitaxel on CMGC in in-vitro and in-vivo settings. Results Paclitaxel...
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-023-03837-4 |
| _version_ | 1797363186124455936 |
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| author | Hyung-Kyu Chae Ye-In Oh Ga-Hyun Lim Yun-Chan Jung Seol-Hee Park Ju-Hyun An Su-Min Park Kyoung-Won Seo Sung-Nam Chu Qiang Li Hwa-Young Youn |
| author_facet | Hyung-Kyu Chae Ye-In Oh Ga-Hyun Lim Yun-Chan Jung Seol-Hee Park Ju-Hyun An Su-Min Park Kyoung-Won Seo Sung-Nam Chu Qiang Li Hwa-Young Youn |
| author_sort | Hyung-Kyu Chae |
| collection | DOAJ |
| description | Abstract Background Canine mammary gland cancer (CMGC) is a common neoplasm in intact bitches. However, the benefit of adjuvant chemotherapy is unclear. The aim of this study was to investigate the anti-proliferative effects of paclitaxel on CMGC in in-vitro and in-vivo settings. Results Paclitaxel dose-dependently inhibited viability and induced G2/M phase cell cycle arrest and apoptosis in both primary and metastatic CMGC cell lines (CIPp and CIPm). In animal experiments, the average tumour volume decreased significantly in proportion to the administered oral paclitaxel dose. By examining tumour tissue using a TUNEL assay and immunohistochemical staining with anti-CD31 as a marker of endothelial differentiation, respectively, it was confirmed that oral paclitaxel induced apoptosis and exerted an anti-angiogenetic effect in tumour tissues. Further, downregulation of cyclin D1 in tumour tissues suggested that oral paclitaxel induced cell cycle arrest in tumour tissues in-vivo. Conclusions Our results suggest that paclitaxel may have anti-cancer effects on CMGC through cell cycle arrest, induction of apoptosis, and anti-angiogenesis. This study could provide a novel approach to treat CMGC. |
| first_indexed | 2024-03-08T16:17:29Z |
| format | Article |
| id | doaj.art-90650f706ab24846919f4320ab38922a |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-03-08T16:17:29Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-90650f706ab24846919f4320ab38922a2024-01-07T12:30:35ZengBMCBMC Veterinary Research1746-61482024-01-0120111110.1186/s12917-023-03837-4Anti-cancer effects of DHP107 on canine mammary gland cancer examined through in-vitro and in-vivo mouse xenograft modelsHyung-Kyu Chae0Ye-In Oh1Ga-Hyun Lim2Yun-Chan Jung3Seol-Hee Park4Ju-Hyun An5Su-Min Park6Kyoung-Won Seo7Sung-Nam Chu8Qiang Li9Hwa-Young Youn10Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National UniversityDepartment of Veterinary Internal Medicine, College of Veterinary Medicine, Kyungpook National UniversityLaboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National UniversityLaboratory Animal Center, CHA University, CHA BiocomplexLaboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National UniversityDepartment of Veterinary Emergency and Critical Care Medicine and Institute of Veterinary Science, College of Veterinary Medicine, Kangwon National UniversityLaboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National UniversityLaboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National UniversityPangyo Research Laboratory, DaeHwa Pharmaceutical Co. LtdDepartment of Veterinary Medicine, College of Agriculture, YanBian UniversityLaboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National UniversityAbstract Background Canine mammary gland cancer (CMGC) is a common neoplasm in intact bitches. However, the benefit of adjuvant chemotherapy is unclear. The aim of this study was to investigate the anti-proliferative effects of paclitaxel on CMGC in in-vitro and in-vivo settings. Results Paclitaxel dose-dependently inhibited viability and induced G2/M phase cell cycle arrest and apoptosis in both primary and metastatic CMGC cell lines (CIPp and CIPm). In animal experiments, the average tumour volume decreased significantly in proportion to the administered oral paclitaxel dose. By examining tumour tissue using a TUNEL assay and immunohistochemical staining with anti-CD31 as a marker of endothelial differentiation, respectively, it was confirmed that oral paclitaxel induced apoptosis and exerted an anti-angiogenetic effect in tumour tissues. Further, downregulation of cyclin D1 in tumour tissues suggested that oral paclitaxel induced cell cycle arrest in tumour tissues in-vivo. Conclusions Our results suggest that paclitaxel may have anti-cancer effects on CMGC through cell cycle arrest, induction of apoptosis, and anti-angiogenesis. This study could provide a novel approach to treat CMGC.https://doi.org/10.1186/s12917-023-03837-4CancerChemotherapyCanineOral paclitaxelMammary gland cancer |
| spellingShingle | Hyung-Kyu Chae Ye-In Oh Ga-Hyun Lim Yun-Chan Jung Seol-Hee Park Ju-Hyun An Su-Min Park Kyoung-Won Seo Sung-Nam Chu Qiang Li Hwa-Young Youn Anti-cancer effects of DHP107 on canine mammary gland cancer examined through in-vitro and in-vivo mouse xenograft models BMC Veterinary Research Cancer Chemotherapy Canine Oral paclitaxel Mammary gland cancer |
| title | Anti-cancer effects of DHP107 on canine mammary gland cancer examined through in-vitro and in-vivo mouse xenograft models |
| title_full | Anti-cancer effects of DHP107 on canine mammary gland cancer examined through in-vitro and in-vivo mouse xenograft models |
| title_fullStr | Anti-cancer effects of DHP107 on canine mammary gland cancer examined through in-vitro and in-vivo mouse xenograft models |
| title_full_unstemmed | Anti-cancer effects of DHP107 on canine mammary gland cancer examined through in-vitro and in-vivo mouse xenograft models |
| title_short | Anti-cancer effects of DHP107 on canine mammary gland cancer examined through in-vitro and in-vivo mouse xenograft models |
| title_sort | anti cancer effects of dhp107 on canine mammary gland cancer examined through in vitro and in vivo mouse xenograft models |
| topic | Cancer Chemotherapy Canine Oral paclitaxel Mammary gland cancer |
| url | https://doi.org/10.1186/s12917-023-03837-4 |
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