Hepatoprotective and antifibrotic activity of watercress extract in a model of CCl4-induced liver fibrosis in Wistar rats
The present study aimed to analyze the antioxidant and hepatoprotective efficacy of watercress extract against liver fibrosis induced by CCl4 (0.8 mL/kg, i.p) in an in vivo rat model. Four groups were included, each with five animals (n = 5): 1) Intact, 2) CCl4-induced for 4 weeks, 3) CCl4-induced f...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623003602 |
| _version_ | 1797660387676520448 |
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| author | Ivonne Peugnet-González Sandra Luz Martínez-Hernández Manuel Enrique Ávila-Blanco David Alejandro Hernández-Marín José Roberto Macias-Pérez Liseth Rubí Aldaba-Muruato Teódulo Quezada-Tristán Joaquín Sosa-Ramírez Gloria Stephanie Villa-Jaimes Javier Ventura-Juárez Martín Muñoz-Ortega David Ibarra-Martínez |
| author_facet | Ivonne Peugnet-González Sandra Luz Martínez-Hernández Manuel Enrique Ávila-Blanco David Alejandro Hernández-Marín José Roberto Macias-Pérez Liseth Rubí Aldaba-Muruato Teódulo Quezada-Tristán Joaquín Sosa-Ramírez Gloria Stephanie Villa-Jaimes Javier Ventura-Juárez Martín Muñoz-Ortega David Ibarra-Martínez |
| author_sort | Ivonne Peugnet-González |
| collection | DOAJ |
| description | The present study aimed to analyze the antioxidant and hepatoprotective efficacy of watercress extract against liver fibrosis induced by CCl4 (0.8 mL/kg, i.p) in an in vivo rat model. Four groups were included, each with five animals (n = 5): 1) Intact, 2) CCl4-induced for 4 weeks, 3) CCl4-induced for 8 weeks, and 4) CCl4-induced for 4 weeks followed by watercress extract treatment (600 mg/kg/day) until week 8. Tissue samples were analyzed using histological techniques (H&E, Sirius Red, Masson's trichrome stain), biochemical methods, and gene expression analysis of IL-1, IL-10, TGF-β, NRF2, Keap1, Acta2, Timp-2, MMP-13, and MMP-9. Watercress extract administration resulted in improvements by preventing steatosis and fibrosis. It also increased IL-10 expression in the liver and intestine. Additionally, the expression of fibrogenic markers such as TGF-β, Col 1α, Acta2, and Timp-2 was regulated. These results suggest that orally administered watercress extract prevents the progression of liver fibrosis. |
| first_indexed | 2024-03-11T18:29:05Z |
| format | Article |
| id | doaj.art-9065feb3e8b4413f8e3c9f61dc43debb |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-11T18:29:05Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-9065feb3e8b4413f8e3c9f61dc43debb2023-10-13T13:52:47ZengElsevierJournal of Functional Foods1756-46462023-10-01109105760Hepatoprotective and antifibrotic activity of watercress extract in a model of CCl4-induced liver fibrosis in Wistar ratsIvonne Peugnet-González0Sandra Luz Martínez-Hernández1Manuel Enrique Ávila-Blanco2David Alejandro Hernández-Marín3José Roberto Macias-Pérez4Liseth Rubí Aldaba-Muruato5Teódulo Quezada-Tristán6Joaquín Sosa-Ramírez7Gloria Stephanie Villa-Jaimes8Javier Ventura-Juárez9Martín Muñoz-Ortega10David Ibarra-Martínez11Department of Morphology, Center for Basic Sciences, Autonomous University of Aguascalientes, Aguascalientes, Ags 20100, MexicoDepartment of Microbiology, Center for Basic Sciences, Autonomous University of Aguascalientes. Aguascalientes, Ags 20100, MexicoDepartment of Morphology, Center for Basic Sciences, Autonomous University of Aguascalientes, Aguascalientes, Ags 20100, MexicoDepartment of Microbiology, Center for Basic Sciences, Autonomous University of Aguascalientes. Aguascalientes, Ags 20100, MexicoFaculty of Professional Studies, Huasteca Zone, Autonomous University of San Luis Potosí. Ciudad Valles, San Luis Potosi 79060, MexicoFaculty of Professional Studies, Huasteca Zone, Autonomous University of San Luis Potosí. Ciudad Valles, San Luis Potosi 79060, MexicoDepartment of Veterinary Sciences, Center for Agricultural Sciences, Autonomous University of Aguascalientes, Aguascalientes, Ags 20100, MexicoDepartment of Agronomy, Center for Agricultural Sciences, Autonomous University of Aguascalientes, Aguascalientes, Ags 20100, MexicoDepartment of Morphology, Center for Basic Sciences, Autonomous University of Aguascalientes, Aguascalientes, Ags 20100, MexicoDepartment of Morphology, Center for Basic Sciences, Autonomous University of Aguascalientes, Aguascalientes, Ags 20100, MexicoDepartment of Chemistry, Center for Basic Sciences, Autonomous University of Aguascalientes, Aguascalientes, Ags 20100, Mexico; Corresponding authors at: Department of Chemistry, Autonomous University of Aguascalientes, 940 University Avenue, Aguascalientes C.P. 20100, México.Department of Chemistry, Center for Basic Sciences, Autonomous University of Aguascalientes, Aguascalientes, Ags 20100, Mexico; Corresponding authors at: Department of Chemistry, Autonomous University of Aguascalientes, 940 University Avenue, Aguascalientes C.P. 20100, México.The present study aimed to analyze the antioxidant and hepatoprotective efficacy of watercress extract against liver fibrosis induced by CCl4 (0.8 mL/kg, i.p) in an in vivo rat model. Four groups were included, each with five animals (n = 5): 1) Intact, 2) CCl4-induced for 4 weeks, 3) CCl4-induced for 8 weeks, and 4) CCl4-induced for 4 weeks followed by watercress extract treatment (600 mg/kg/day) until week 8. Tissue samples were analyzed using histological techniques (H&E, Sirius Red, Masson's trichrome stain), biochemical methods, and gene expression analysis of IL-1, IL-10, TGF-β, NRF2, Keap1, Acta2, Timp-2, MMP-13, and MMP-9. Watercress extract administration resulted in improvements by preventing steatosis and fibrosis. It also increased IL-10 expression in the liver and intestine. Additionally, the expression of fibrogenic markers such as TGF-β, Col 1α, Acta2, and Timp-2 was regulated. These results suggest that orally administered watercress extract prevents the progression of liver fibrosis.http://www.sciencedirect.com/science/article/pii/S1756464623003602Hepatoprotective activityInterleukinLiver fibrosisOxidative stressWatercress extract |
| spellingShingle | Ivonne Peugnet-González Sandra Luz Martínez-Hernández Manuel Enrique Ávila-Blanco David Alejandro Hernández-Marín José Roberto Macias-Pérez Liseth Rubí Aldaba-Muruato Teódulo Quezada-Tristán Joaquín Sosa-Ramírez Gloria Stephanie Villa-Jaimes Javier Ventura-Juárez Martín Muñoz-Ortega David Ibarra-Martínez Hepatoprotective and antifibrotic activity of watercress extract in a model of CCl4-induced liver fibrosis in Wistar rats Journal of Functional Foods Hepatoprotective activity Interleukin Liver fibrosis Oxidative stress Watercress extract |
| title | Hepatoprotective and antifibrotic activity of watercress extract in a model of CCl4-induced liver fibrosis in Wistar rats |
| title_full | Hepatoprotective and antifibrotic activity of watercress extract in a model of CCl4-induced liver fibrosis in Wistar rats |
| title_fullStr | Hepatoprotective and antifibrotic activity of watercress extract in a model of CCl4-induced liver fibrosis in Wistar rats |
| title_full_unstemmed | Hepatoprotective and antifibrotic activity of watercress extract in a model of CCl4-induced liver fibrosis in Wistar rats |
| title_short | Hepatoprotective and antifibrotic activity of watercress extract in a model of CCl4-induced liver fibrosis in Wistar rats |
| title_sort | hepatoprotective and antifibrotic activity of watercress extract in a model of ccl4 induced liver fibrosis in wistar rats |
| topic | Hepatoprotective activity Interleukin Liver fibrosis Oxidative stress Watercress extract |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623003602 |
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