Investigation of Genetic Diversity of Pasteurella multocida Isolated from Diseased Poultry in Korea
ABSTRACT Pasteurella multocida causes fowl cholera which is an economically important disease in poultry industries around the world. In this study, we analyzed the capsular genotype, lipopolysaccharide (LPS) genotype, virulence-associated genes (VAGs) patterns, antimicrobial resistance and genetic...
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| Format: | Article |
| Language: | English |
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Fundação APINCO de Ciência e Tecnologia Avícolas
2021-06-01
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| Series: | Brazilian Journal of Poultry Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000200306&tlng=en |
| _version_ | 1818988665942048768 |
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| author | J Jeong MS Kang OM Jeong HJ Lee JY Lee YK Kwon JW Park JH Kim |
| author_facet | J Jeong MS Kang OM Jeong HJ Lee JY Lee YK Kwon JW Park JH Kim |
| author_sort | J Jeong |
| collection | DOAJ |
| description | ABSTRACT Pasteurella multocida causes fowl cholera which is an economically important disease in poultry industries around the world. In this study, we analyzed the capsular genotype, lipopolysaccharide (LPS) genotype, virulence-associated genes (VAGs) patterns, antimicrobial resistance and genetic diversity in a total of 9 P. multocida isolates from poultry with fowl cholera between 2014 and 2019 in Korea. When combining the capsular types with the LPS genotypes, two isolates of the 9 isolates were A:L3, and the others were non-typeable (NT): L3. Of the 23 VAGs, all the isolates harbored ptfA, fimA, hsf-1, hsf-2, pfhA, exbB, exbD, tonB, hgbA, hgbB, fur, sodA, sodC, pmHAS, ompA, ompH, oma87, plpB, psl, and nanH, whereas toxA gene was not detected in any of the 9 isolates. In addition, among the 11 antimicrobials, most of the isolates except for one isolate resistant to florfenicol, exhibited susceptibility to all the antimicrobials. Multi-locus sequence typing (MLST) analysis revealed 5 different sequence types (ST): ST8, ST351, ST352, ST353, and ST368. The ST351, ST352, ST353, and ST368 were identified for the first time in this study, and ST352 and ST353 isolates were largely prevalent nationwide. These STs isolates should be monitored continuously because in some cases, ST352 and ST353 isolates demonstrated high mortality rates. Although only limited numbers of isolates have been analyzed, our findings provide overall characteristics and epidemiological information of the P. multocida strains recently prevalent in Korea. |
| first_indexed | 2024-12-20T19:26:12Z |
| format | Article |
| id | doaj.art-90665685e0224497ad3035520d6b9383 |
| institution | Directory Open Access Journal |
| issn | 1806-9061 |
| language | English |
| last_indexed | 2024-12-20T19:26:12Z |
| publishDate | 2021-06-01 |
| publisher | Fundação APINCO de Ciência e Tecnologia Avícolas |
| record_format | Article |
| series | Brazilian Journal of Poultry Science |
| spelling | doaj.art-90665685e0224497ad3035520d6b93832022-12-21T19:28:52ZengFundação APINCO de Ciência e Tecnologia AvícolasBrazilian Journal of Poultry Science1806-90612021-06-0123210.1590/1806-9061-2020-1390Investigation of Genetic Diversity of Pasteurella multocida Isolated from Diseased Poultry in KoreaJ Jeonghttps://orcid.org/0000-0001-6228-7164MS Kanghttps://orcid.org/0000-0002-8983-4129OM Jeonghttps://orcid.org/0000-0003-2159-2693HJ Leehttps://orcid.org/0000-0003-2097-3064JY Leehttps://orcid.org/0000-0003-2410-8115YK Kwonhttps://orcid.org/0000-0003-2435-7330JW Parkhttps://orcid.org/0000-0002-2546-9732JH Kimhttps://orcid.org/0000-0002-5464-0447ABSTRACT Pasteurella multocida causes fowl cholera which is an economically important disease in poultry industries around the world. In this study, we analyzed the capsular genotype, lipopolysaccharide (LPS) genotype, virulence-associated genes (VAGs) patterns, antimicrobial resistance and genetic diversity in a total of 9 P. multocida isolates from poultry with fowl cholera between 2014 and 2019 in Korea. When combining the capsular types with the LPS genotypes, two isolates of the 9 isolates were A:L3, and the others were non-typeable (NT): L3. Of the 23 VAGs, all the isolates harbored ptfA, fimA, hsf-1, hsf-2, pfhA, exbB, exbD, tonB, hgbA, hgbB, fur, sodA, sodC, pmHAS, ompA, ompH, oma87, plpB, psl, and nanH, whereas toxA gene was not detected in any of the 9 isolates. In addition, among the 11 antimicrobials, most of the isolates except for one isolate resistant to florfenicol, exhibited susceptibility to all the antimicrobials. Multi-locus sequence typing (MLST) analysis revealed 5 different sequence types (ST): ST8, ST351, ST352, ST353, and ST368. The ST351, ST352, ST353, and ST368 were identified for the first time in this study, and ST352 and ST353 isolates were largely prevalent nationwide. These STs isolates should be monitored continuously because in some cases, ST352 and ST353 isolates demonstrated high mortality rates. Although only limited numbers of isolates have been analyzed, our findings provide overall characteristics and epidemiological information of the P. multocida strains recently prevalent in Korea.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000200306&tlng=enAntimicrobial susceptibility testfowl choleramulti-locus sequence type (MLST)Pasteurella multocidavirulence-associated gene (VAG) |
| spellingShingle | J Jeong MS Kang OM Jeong HJ Lee JY Lee YK Kwon JW Park JH Kim Investigation of Genetic Diversity of Pasteurella multocida Isolated from Diseased Poultry in Korea Brazilian Journal of Poultry Science Antimicrobial susceptibility test fowl cholera multi-locus sequence type (MLST) Pasteurella multocida virulence-associated gene (VAG) |
| title | Investigation of Genetic Diversity of Pasteurella multocida Isolated from Diseased Poultry in Korea |
| title_full | Investigation of Genetic Diversity of Pasteurella multocida Isolated from Diseased Poultry in Korea |
| title_fullStr | Investigation of Genetic Diversity of Pasteurella multocida Isolated from Diseased Poultry in Korea |
| title_full_unstemmed | Investigation of Genetic Diversity of Pasteurella multocida Isolated from Diseased Poultry in Korea |
| title_short | Investigation of Genetic Diversity of Pasteurella multocida Isolated from Diseased Poultry in Korea |
| title_sort | investigation of genetic diversity of pasteurella multocida isolated from diseased poultry in korea |
| topic | Antimicrobial susceptibility test fowl cholera multi-locus sequence type (MLST) Pasteurella multocida virulence-associated gene (VAG) |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000200306&tlng=en |
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