Microbial community in resistant and susceptible Churra sheep infected by Teladorsagia circumcincta
Abstract Gastrointestinal nematodes (GIN) are a major threat to health and welfare in small ruminants worldwide. Teladorsagia circumcincta is a nematode that inhabits the abomasum of sheep, especially in temperate regions, causing important economic losses. Given that T. circumcincta and microbiome...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-21058-x |
| _version_ | 1797986030148648960 |
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| author | Verónica Castilla Gómez de Agüero Cristina Esteban-Blanco Héctor Argüello Elora Valderas-García Sonia Andrés Rafael Balaña-Fouce Juan José Arranz Beatriz Gutiérrez-Gil María Martínez-Valladares |
| author_facet | Verónica Castilla Gómez de Agüero Cristina Esteban-Blanco Héctor Argüello Elora Valderas-García Sonia Andrés Rafael Balaña-Fouce Juan José Arranz Beatriz Gutiérrez-Gil María Martínez-Valladares |
| author_sort | Verónica Castilla Gómez de Agüero |
| collection | DOAJ |
| description | Abstract Gastrointestinal nematodes (GIN) are a major threat to health and welfare in small ruminants worldwide. Teladorsagia circumcincta is a nematode that inhabits the abomasum of sheep, especially in temperate regions, causing important economic losses. Given that T. circumcincta and microbiome share the same niche, interactions between them and the host are expected. Although it is known that within a sheep breed there are animals that are more resistant than others to infection by GIN, it is not known if the microbiome influences the phenotype of these animals. Under this condition, 12 sheep were classified according to their cumulative faecal egg count (cFEC) at the end of a first experimental infection, 6 as resistant group (RG) and 6 as susceptible group (SG) to T. circumcincta infection. Then, all sheep were experimentally infected with 70,000 L3 of T. circumcincta and at day 7 days post-infection were euthanized. At necropsy, gastric mucosa and gastric content from abomasum were collected to extract bacterial DNA and sequence V3-V4 region from 16S rRNA gene using Ilumina technology. After bioanalysis performed, results showed that α-diversity and β-diversity remained similar in both groups. However, resistant phenotype sheep showed a higher number of bacteria butyrate-fermenting species as Clostridium sensu stricto 1 (abundance in RG: 1.29% and in SG: 0.069%; p = 0.05), and Turicibacter (abundance in RG: 0.31% and in SG: 0.027%; p = 0.07) in gastric content but also Serratia spp in gastric mucosa (abundance in RG: 0.12% and in SG: 0.041%; p = 0.07). A trend towards a significant negative correlation between cFEC and Clostridium sensu stricto 1 abundance in gastric content was detected (r = − 0.537; p = 0.08). These data suggest that microbiome composition could be another factor associated with the development of the resistant phenotype modifying the interaction with the host and the in last instance affecting the individual risk of infection. |
| first_indexed | 2024-04-11T07:27:48Z |
| format | Article |
| id | doaj.art-90671d93bd9341b2bb6dd46bb0148c29 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T07:27:48Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-90671d93bd9341b2bb6dd46bb0148c292022-12-22T04:37:02ZengNature PortfolioScientific Reports2045-23222022-10-0112111110.1038/s41598-022-21058-xMicrobial community in resistant and susceptible Churra sheep infected by Teladorsagia circumcinctaVerónica Castilla Gómez de Agüero0Cristina Esteban-Blanco1Héctor Argüello2Elora Valderas-García3Sonia Andrés4Rafael Balaña-Fouce5Juan José Arranz6Beatriz Gutiérrez-Gil7María Martínez-Valladares8Instituto de Ganadería de Montaña, CSIC-Universidad de LeónDepartamento de Producción Animal, Facultad de Veterinaria, Universidad de LeónDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad de LeónInstituto de Ganadería de Montaña, CSIC-Universidad de LeónInstituto de Ganadería de Montaña, CSIC-Universidad de LeónDepartamento de Ciencias Biomédicas, Facultad de Veterinaria, Universidad de LeónDepartamento de Producción Animal, Facultad de Veterinaria, Universidad de LeónDepartamento de Producción Animal, Facultad de Veterinaria, Universidad de LeónInstituto de Ganadería de Montaña, CSIC-Universidad de LeónAbstract Gastrointestinal nematodes (GIN) are a major threat to health and welfare in small ruminants worldwide. Teladorsagia circumcincta is a nematode that inhabits the abomasum of sheep, especially in temperate regions, causing important economic losses. Given that T. circumcincta and microbiome share the same niche, interactions between them and the host are expected. Although it is known that within a sheep breed there are animals that are more resistant than others to infection by GIN, it is not known if the microbiome influences the phenotype of these animals. Under this condition, 12 sheep were classified according to their cumulative faecal egg count (cFEC) at the end of a first experimental infection, 6 as resistant group (RG) and 6 as susceptible group (SG) to T. circumcincta infection. Then, all sheep were experimentally infected with 70,000 L3 of T. circumcincta and at day 7 days post-infection were euthanized. At necropsy, gastric mucosa and gastric content from abomasum were collected to extract bacterial DNA and sequence V3-V4 region from 16S rRNA gene using Ilumina technology. After bioanalysis performed, results showed that α-diversity and β-diversity remained similar in both groups. However, resistant phenotype sheep showed a higher number of bacteria butyrate-fermenting species as Clostridium sensu stricto 1 (abundance in RG: 1.29% and in SG: 0.069%; p = 0.05), and Turicibacter (abundance in RG: 0.31% and in SG: 0.027%; p = 0.07) in gastric content but also Serratia spp in gastric mucosa (abundance in RG: 0.12% and in SG: 0.041%; p = 0.07). A trend towards a significant negative correlation between cFEC and Clostridium sensu stricto 1 abundance in gastric content was detected (r = − 0.537; p = 0.08). These data suggest that microbiome composition could be another factor associated with the development of the resistant phenotype modifying the interaction with the host and the in last instance affecting the individual risk of infection.https://doi.org/10.1038/s41598-022-21058-x |
| spellingShingle | Verónica Castilla Gómez de Agüero Cristina Esteban-Blanco Héctor Argüello Elora Valderas-García Sonia Andrés Rafael Balaña-Fouce Juan José Arranz Beatriz Gutiérrez-Gil María Martínez-Valladares Microbial community in resistant and susceptible Churra sheep infected by Teladorsagia circumcincta Scientific Reports |
| title | Microbial community in resistant and susceptible Churra sheep infected by Teladorsagia circumcincta |
| title_full | Microbial community in resistant and susceptible Churra sheep infected by Teladorsagia circumcincta |
| title_fullStr | Microbial community in resistant and susceptible Churra sheep infected by Teladorsagia circumcincta |
| title_full_unstemmed | Microbial community in resistant and susceptible Churra sheep infected by Teladorsagia circumcincta |
| title_short | Microbial community in resistant and susceptible Churra sheep infected by Teladorsagia circumcincta |
| title_sort | microbial community in resistant and susceptible churra sheep infected by teladorsagia circumcincta |
| url | https://doi.org/10.1038/s41598-022-21058-x |
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