Changes in global DNA methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposure
Objective This prospective clinical study comparatively investigated the effects of tobacco smoking on global methylation and hydroxymethylation in oral epithelial cells. Methods Buccal cells from the inside of the cheeks were collected from 47 individuals, including smokers, former smokers, and nev...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-09-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/0300060520954677 |
| _version_ | 1819030688328843264 |
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| author | Gabriela-Emilia Avram Anca Marcu Alexandra Moatar Corina Samoila Angela Podariu Edward Seclaman Catalin Marian |
| author_facet | Gabriela-Emilia Avram Anca Marcu Alexandra Moatar Corina Samoila Angela Podariu Edward Seclaman Catalin Marian |
| author_sort | Gabriela-Emilia Avram |
| collection | DOAJ |
| description | Objective This prospective clinical study comparatively investigated the effects of tobacco smoking on global methylation and hydroxymethylation in oral epithelial cells. Methods Buccal cells from the inside of the cheeks were collected from 47 individuals, including smokers, former smokers, and never smokers. DNA was extracted using dedicated kits. Methylated and hydroxymethylated DNA fractions were measured using assays similar to enzyme-linked immunosorbent assays. The levels of methylation and hydroxymethylation were compared among groups using unpaired two-tailed t-tests or the Mann–Whitney U test; P < 0.05 was considered statistically significant. Results There was no statistically significant difference in the average number of cigarettes between smoker and former smoker groups. Although methylation levels were lower for smokers (3.1%) and former smokers (2.16%), compared with never smokers (4.16%), these differences were not statistically significant. There was a two-fold increase in hydroxymethylation level in never smokers, compared with smokers. Conclusions Our findings suggest that smoking leads to global reductions in both methylation and hydroxymethylation levels in oral epithelial cells in a manner influenced by the intensity and length of exposure to tobacco smoke. |
| first_indexed | 2024-12-21T06:34:08Z |
| format | Article |
| id | doaj.art-906ab7769b2046daaa7aba3b5c96059d |
| institution | Directory Open Access Journal |
| issn | 1473-2300 |
| language | English |
| last_indexed | 2024-12-21T06:34:08Z |
| publishDate | 2020-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj.art-906ab7769b2046daaa7aba3b5c96059d2022-12-21T19:12:56ZengSAGE PublishingJournal of International Medical Research1473-23002020-09-014810.1177/0300060520954677Changes in global DNA methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposureGabriela-Emilia AvramAnca MarcuAlexandra MoatarCorina SamoilaAngela PodariuEdward SeclamanCatalin MarianObjective This prospective clinical study comparatively investigated the effects of tobacco smoking on global methylation and hydroxymethylation in oral epithelial cells. Methods Buccal cells from the inside of the cheeks were collected from 47 individuals, including smokers, former smokers, and never smokers. DNA was extracted using dedicated kits. Methylated and hydroxymethylated DNA fractions were measured using assays similar to enzyme-linked immunosorbent assays. The levels of methylation and hydroxymethylation were compared among groups using unpaired two-tailed t-tests or the Mann–Whitney U test; P < 0.05 was considered statistically significant. Results There was no statistically significant difference in the average number of cigarettes between smoker and former smoker groups. Although methylation levels were lower for smokers (3.1%) and former smokers (2.16%), compared with never smokers (4.16%), these differences were not statistically significant. There was a two-fold increase in hydroxymethylation level in never smokers, compared with smokers. Conclusions Our findings suggest that smoking leads to global reductions in both methylation and hydroxymethylation levels in oral epithelial cells in a manner influenced by the intensity and length of exposure to tobacco smoke.https://doi.org/10.1177/0300060520954677 |
| spellingShingle | Gabriela-Emilia Avram Anca Marcu Alexandra Moatar Corina Samoila Angela Podariu Edward Seclaman Catalin Marian Changes in global DNA methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposure Journal of International Medical Research |
| title | Changes in global DNA methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposure |
| title_full | Changes in global DNA methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposure |
| title_fullStr | Changes in global DNA methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposure |
| title_full_unstemmed | Changes in global DNA methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposure |
| title_short | Changes in global DNA methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposure |
| title_sort | changes in global dna methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposure |
| url | https://doi.org/10.1177/0300060520954677 |
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