Functional Anatomical Changes in Ulcerative Colitis Patients Determine Their Gut Microbiota Composition and Consequently the Possible Treatment Outcome
Gut microbial composition alters in some special situations, such as in ulcerative colits (UC) after total proctocolectomy and ileal pouch-anal anastomosis (IPAA) surgery. The aim of our study was to determine the composition of the intestinal microbiome in UC patients after IPAA surgery, compared w...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-10-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/13/11/346 |
| _version_ | 1827703422942445568 |
|---|---|
| author | Anita Bálint Klaudia Farkas Orsolya Méhi Bálint Kintses Bálint Márk Vásárhelyi Eszter Ari Csaba Pál Tamara Madácsy József Maléth Kata Judit Szántó István Nagy Mariann Rutka Péter Bacsur Diána Szűcs Zoltán Szepes Ferenc Nagy Anna Fábián Renáta Bor Ágnes Milassin Tamás Molnár |
| author_facet | Anita Bálint Klaudia Farkas Orsolya Méhi Bálint Kintses Bálint Márk Vásárhelyi Eszter Ari Csaba Pál Tamara Madácsy József Maléth Kata Judit Szántó István Nagy Mariann Rutka Péter Bacsur Diána Szűcs Zoltán Szepes Ferenc Nagy Anna Fábián Renáta Bor Ágnes Milassin Tamás Molnár |
| author_sort | Anita Bálint |
| collection | DOAJ |
| description | Gut microbial composition alters in some special situations, such as in ulcerative colits (UC) after total proctocolectomy and ileal pouch-anal anastomosis (IPAA) surgery. The aim of our study was to determine the composition of the intestinal microbiome in UC patients after IPAA surgery, compared with UC patients, familial adenomatous polyposis (FAP) patients after IPAA surgery and healthy controls. Clinical data of patients, blood and faecal samples were collected. Faecal microbiota structure was determined by sequencing the V4 hypervariable region of the 16S rRNA gene. Overall, 56 patients were enrolled. Compared to the Healthy group, both the Pouch active and UC active groups had higher <i>Enterobacteriaceae</i>, <i>Enterococcaceae</i> and <i>Pasteurellaceae</i> abundance. The Pouch and UC groups showed distinct separation based on their alpha and beta bacterial diversities. The UC group had higher <i>Prevotellaceae</i>, <i>Rikenellaceae</i>, <i>Ruminococcaceae</i> abundance compared to the Pouch active group. Pouch and FAP participants showed similar bacterial community composition. There was no significant difference in the bacterial abundance between the active and inactive subgroups of the Pouch or UC groups. Gut microbiome and anatomical status together construct a functional unit that has influence on diversity, in addition to intestinal inflammation that is a part of the pathomechanism in UC. |
| first_indexed | 2024-03-10T15:17:59Z |
| format | Article |
| id | doaj.art-906b98ae485b475f9f966b5b93e82539 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T15:17:59Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-906b98ae485b475f9f966b5b93e825392023-11-20T18:47:50ZengMDPI AGPharmaceuticals1424-82472020-10-01131134610.3390/ph13110346Functional Anatomical Changes in Ulcerative Colitis Patients Determine Their Gut Microbiota Composition and Consequently the Possible Treatment OutcomeAnita Bálint0Klaudia Farkas1Orsolya Méhi2Bálint Kintses3Bálint Márk Vásárhelyi4Eszter Ari5Csaba Pál6Tamara Madácsy7József Maléth8Kata Judit Szántó9István Nagy10Mariann Rutka11Péter Bacsur12Diána Szűcs13Zoltán Szepes14Ferenc Nagy15Anna Fábián16Renáta Bor17Ágnes Milassin18Tamás Molnár19Department of Medicine, University of Szeged, 6720 Szeged, HungaryDepartment of Medicine, University of Szeged, 6720 Szeged, HungarySynthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, 6726 Szeged, HungarySynthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, 6726 Szeged, HungarySynthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, 6726 Szeged, HungarySynthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, 6726 Szeged, HungarySynthetic and Systems Biology Unit, Institute of Biochemistry, Biological Research Centre, 6726 Szeged, HungaryDepartment of Medicine, University of Szeged, 6720 Szeged, HungaryDepartment of Medicine, University of Szeged, 6720 Szeged, HungaryDepartment of Medicine, University of Szeged, 6720 Szeged, HungarySequencing Platform, Institute of Biochemistry, Biological Research Centre, 6726 Szeged, HungaryDepartment of Medicine, University of Szeged, 6720 Szeged, HungaryDepartment of Medicine, University of Szeged, 6720 Szeged, HungaryDepartment of Medicine, University of Szeged, 6720 Szeged, HungaryDepartment of Medicine, University of Szeged, 6720 Szeged, HungaryDepartment of Medicine, University of Szeged, 6720 Szeged, HungaryDepartment of Medicine, University of Szeged, 6720 Szeged, HungaryDepartment of Medicine, University of Szeged, 6720 Szeged, HungaryDepartment of Medicine, University of Szeged, 6720 Szeged, HungaryDepartment of Medicine, University of Szeged, 6720 Szeged, HungaryGut microbial composition alters in some special situations, such as in ulcerative colits (UC) after total proctocolectomy and ileal pouch-anal anastomosis (IPAA) surgery. The aim of our study was to determine the composition of the intestinal microbiome in UC patients after IPAA surgery, compared with UC patients, familial adenomatous polyposis (FAP) patients after IPAA surgery and healthy controls. Clinical data of patients, blood and faecal samples were collected. Faecal microbiota structure was determined by sequencing the V4 hypervariable region of the 16S rRNA gene. Overall, 56 patients were enrolled. Compared to the Healthy group, both the Pouch active and UC active groups had higher <i>Enterobacteriaceae</i>, <i>Enterococcaceae</i> and <i>Pasteurellaceae</i> abundance. The Pouch and UC groups showed distinct separation based on their alpha and beta bacterial diversities. The UC group had higher <i>Prevotellaceae</i>, <i>Rikenellaceae</i>, <i>Ruminococcaceae</i> abundance compared to the Pouch active group. Pouch and FAP participants showed similar bacterial community composition. There was no significant difference in the bacterial abundance between the active and inactive subgroups of the Pouch or UC groups. Gut microbiome and anatomical status together construct a functional unit that has influence on diversity, in addition to intestinal inflammation that is a part of the pathomechanism in UC.https://www.mdpi.com/1424-8247/13/11/346gut microbiomefaecal microbiomeulcerative colitispouchfamilial adenomatous polyposisfunctional anatomical changes |
| spellingShingle | Anita Bálint Klaudia Farkas Orsolya Méhi Bálint Kintses Bálint Márk Vásárhelyi Eszter Ari Csaba Pál Tamara Madácsy József Maléth Kata Judit Szántó István Nagy Mariann Rutka Péter Bacsur Diána Szűcs Zoltán Szepes Ferenc Nagy Anna Fábián Renáta Bor Ágnes Milassin Tamás Molnár Functional Anatomical Changes in Ulcerative Colitis Patients Determine Their Gut Microbiota Composition and Consequently the Possible Treatment Outcome Pharmaceuticals gut microbiome faecal microbiome ulcerative colitis pouch familial adenomatous polyposis functional anatomical changes |
| title | Functional Anatomical Changes in Ulcerative Colitis Patients Determine Their Gut Microbiota Composition and Consequently the Possible Treatment Outcome |
| title_full | Functional Anatomical Changes in Ulcerative Colitis Patients Determine Their Gut Microbiota Composition and Consequently the Possible Treatment Outcome |
| title_fullStr | Functional Anatomical Changes in Ulcerative Colitis Patients Determine Their Gut Microbiota Composition and Consequently the Possible Treatment Outcome |
| title_full_unstemmed | Functional Anatomical Changes in Ulcerative Colitis Patients Determine Their Gut Microbiota Composition and Consequently the Possible Treatment Outcome |
| title_short | Functional Anatomical Changes in Ulcerative Colitis Patients Determine Their Gut Microbiota Composition and Consequently the Possible Treatment Outcome |
| title_sort | functional anatomical changes in ulcerative colitis patients determine their gut microbiota composition and consequently the possible treatment outcome |
| topic | gut microbiome faecal microbiome ulcerative colitis pouch familial adenomatous polyposis functional anatomical changes |
| url | https://www.mdpi.com/1424-8247/13/11/346 |
| work_keys_str_mv | AT anitabalint functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT klaudiafarkas functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT orsolyamehi functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT balintkintses functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT balintmarkvasarhelyi functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT eszterari functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT csabapal functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT tamaramadacsy functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT jozsefmaleth functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT katajuditszanto functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT istvannagy functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT mariannrutka functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT peterbacsur functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT dianaszucs functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT zoltanszepes functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT ferencnagy functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT annafabian functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT renatabor functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT agnesmilassin functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome AT tamasmolnar functionalanatomicalchangesinulcerativecolitispatientsdeterminetheirgutmicrobiotacompositionandconsequentlythepossibletreatmentoutcome |