Surface frustration re-patterning underlies the structural landscape and evolvability of fungal orphan candidate effectors
Abstract Pathogens secrete effector proteins to subvert host physiology and cause disease. Effectors are engaged in a molecular arms race with the host resulting in conflicting evolutionary constraints to manipulate host cells without triggering immune responses. The molecular mechanisms allowing ef...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-40949-9 |
| _version_ | 1797558531062235136 |
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| author | Mark C. Derbyshire Sylvain Raffaele |
| author_facet | Mark C. Derbyshire Sylvain Raffaele |
| author_sort | Mark C. Derbyshire |
| collection | DOAJ |
| description | Abstract Pathogens secrete effector proteins to subvert host physiology and cause disease. Effectors are engaged in a molecular arms race with the host resulting in conflicting evolutionary constraints to manipulate host cells without triggering immune responses. The molecular mechanisms allowing effectors to be at the same time robust and evolvable remain largely enigmatic. Here, we show that 62 conserved structure-related families encompass the majority of fungal orphan effector candidates in the Pezizomycotina subphylum. These effectors diversified through changes in patterns of thermodynamic frustration at surface residues. The underlying mutations tended to increase the robustness of the overall effector protein structure while switching potential binding interfaces. This mechanism could explain how conserved effector families maintained biological activity over long evolutionary timespans in different host environments and provides a model for the emergence of sequence-unrelated effector families with conserved structures. |
| first_indexed | 2024-03-10T17:32:44Z |
| format | Article |
| id | doaj.art-906f45af7b6a47169bcd31e0f4f07d7f |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:32:44Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-906f45af7b6a47169bcd31e0f4f07d7f2023-11-20T09:58:07ZengNature PortfolioNature Communications2041-17232023-08-0114111310.1038/s41467-023-40949-9Surface frustration re-patterning underlies the structural landscape and evolvability of fungal orphan candidate effectorsMark C. Derbyshire0Sylvain Raffaele1Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin UniversityLaboratoire des Interactions Plantes Micro-organismes Environnement (LIPME), INRAE, CNRS, Université de ToulouseAbstract Pathogens secrete effector proteins to subvert host physiology and cause disease. Effectors are engaged in a molecular arms race with the host resulting in conflicting evolutionary constraints to manipulate host cells without triggering immune responses. The molecular mechanisms allowing effectors to be at the same time robust and evolvable remain largely enigmatic. Here, we show that 62 conserved structure-related families encompass the majority of fungal orphan effector candidates in the Pezizomycotina subphylum. These effectors diversified through changes in patterns of thermodynamic frustration at surface residues. The underlying mutations tended to increase the robustness of the overall effector protein structure while switching potential binding interfaces. This mechanism could explain how conserved effector families maintained biological activity over long evolutionary timespans in different host environments and provides a model for the emergence of sequence-unrelated effector families with conserved structures.https://doi.org/10.1038/s41467-023-40949-9 |
| spellingShingle | Mark C. Derbyshire Sylvain Raffaele Surface frustration re-patterning underlies the structural landscape and evolvability of fungal orphan candidate effectors Nature Communications |
| title | Surface frustration re-patterning underlies the structural landscape and evolvability of fungal orphan candidate effectors |
| title_full | Surface frustration re-patterning underlies the structural landscape and evolvability of fungal orphan candidate effectors |
| title_fullStr | Surface frustration re-patterning underlies the structural landscape and evolvability of fungal orphan candidate effectors |
| title_full_unstemmed | Surface frustration re-patterning underlies the structural landscape and evolvability of fungal orphan candidate effectors |
| title_short | Surface frustration re-patterning underlies the structural landscape and evolvability of fungal orphan candidate effectors |
| title_sort | surface frustration re patterning underlies the structural landscape and evolvability of fungal orphan candidate effectors |
| url | https://doi.org/10.1038/s41467-023-40949-9 |
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