The effects of ketamine on viability, primary DNA damage, and oxidative stress parameters in HepG2 and SH-SY5Y cells
Ketamine is a dissociative anaesthetic used to induce general anaesthesia in humans and laboratory animals. Due to its hallucinogenic and dissociative effects, it is also used as a recreational drug. Anaesthetic agents can cause toxic effects at the cellular level and affect cell survival, induce DN...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2023-06-01
|
| Series: | Arhiv za Higijenu Rada i Toksikologiju |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/aiht-2023-74-3727 |
| _version_ | 1797693219977297920 |
|---|---|
| author | Jurič Andreja Lovaković Blanka Tariba Zandona Antonio Rašić Dubravka Češi Martin Pizent Alica Neuberg Marijana Canjuga Irena Katalinić Maja Vrdoljak Ana Lucić Rešić Arnes Karačonji Irena Brčić |
| author_facet | Jurič Andreja Lovaković Blanka Tariba Zandona Antonio Rašić Dubravka Češi Martin Pizent Alica Neuberg Marijana Canjuga Irena Katalinić Maja Vrdoljak Ana Lucić Rešić Arnes Karačonji Irena Brčić |
| author_sort | Jurič Andreja |
| collection | DOAJ |
| description | Ketamine is a dissociative anaesthetic used to induce general anaesthesia in humans and laboratory animals. Due to its hallucinogenic and dissociative effects, it is also used as a recreational drug. Anaesthetic agents can cause toxic effects at the cellular level and affect cell survival, induce DNA damage, and cause oxidant/antioxidant imbalance. The aim of this study was to explore these possible adverse effects of ketamine on hepatocellular HepG2 and neuroblastoma SH-SY5Y cells after 24-hour exposure to a concentration range covering concentrations used in analgesia, drug abuse, and anaesthesia (0.39, 1.56, and 6.25 µmol/L, respectively). At these concentrations ketamine had relatively low toxic outcomes, as it lowered HepG2 and SH-SY5Y cell viability up to 30 %, and low, potentially repairable DNA damage. Interestingly, the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione (GSH) remained unchanged in both cell lines. On the other hand, oxidative stress markers [superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT)] pointed to ketamine-induced oxidant/antioxidant imbalance. |
| first_indexed | 2024-03-12T02:40:25Z |
| format | Article |
| id | doaj.art-906fd7f173774025bcf4220b6e4041db |
| institution | Directory Open Access Journal |
| issn | 1848-6312 |
| language | English |
| last_indexed | 2024-03-12T02:40:25Z |
| publishDate | 2023-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Arhiv za Higijenu Rada i Toksikologiju |
| spelling | doaj.art-906fd7f173774025bcf4220b6e4041db2023-09-04T07:10:49ZengSciendoArhiv za Higijenu Rada i Toksikologiju1848-63122023-06-0174210611410.2478/aiht-2023-74-3727The effects of ketamine on viability, primary DNA damage, and oxidative stress parameters in HepG2 and SH-SY5Y cellsJurič Andreja0Lovaković Blanka Tariba1Zandona Antonio2Rašić Dubravka3Češi Martin4Pizent Alica5Neuberg Marijana6Canjuga Irena7Katalinić Maja8Vrdoljak Ana Lucić9Rešić Arnes10Karačonji Irena Brčić111Institute for Medical Research and Occupational Health, Zagreb, Croatia1Institute for Medical Research and Occupational Health, Zagreb, Croatia1Institute for Medical Research and Occupational Health, Zagreb, Croatia1Institute for Medical Research and Occupational Health, Zagreb, Croatia2University of Zagreb Faculty of Food Technology and Biotechnology, Zagreb, Croatia1Institute for Medical Research and Occupational Health, Zagreb, Croatia3University North, University Centre Varaždin, Varaždin, Croatia3University North, University Centre Varaždin, Varaždin, Croatia1Institute for Medical Research and Occupational Health, Zagreb, Croatia1Institute for Medical Research and Occupational Health, Zagreb, Croatia4Children’s Hospital Zagreb Department of Paediatrics, Zagreb, Croatia1Institute for Medical Research and Occupational Health, Zagreb, CroatiaKetamine is a dissociative anaesthetic used to induce general anaesthesia in humans and laboratory animals. Due to its hallucinogenic and dissociative effects, it is also used as a recreational drug. Anaesthetic agents can cause toxic effects at the cellular level and affect cell survival, induce DNA damage, and cause oxidant/antioxidant imbalance. The aim of this study was to explore these possible adverse effects of ketamine on hepatocellular HepG2 and neuroblastoma SH-SY5Y cells after 24-hour exposure to a concentration range covering concentrations used in analgesia, drug abuse, and anaesthesia (0.39, 1.56, and 6.25 µmol/L, respectively). At these concentrations ketamine had relatively low toxic outcomes, as it lowered HepG2 and SH-SY5Y cell viability up to 30 %, and low, potentially repairable DNA damage. Interestingly, the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione (GSH) remained unchanged in both cell lines. On the other hand, oxidative stress markers [superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT)] pointed to ketamine-induced oxidant/antioxidant imbalance.https://doi.org/10.2478/aiht-2023-74-3727anaestheticantioxidant enzymesdrug abuserostoxicityanestezijaantioksidacijski enzimirostoksičnostzlouporaba droga |
| spellingShingle | Jurič Andreja Lovaković Blanka Tariba Zandona Antonio Rašić Dubravka Češi Martin Pizent Alica Neuberg Marijana Canjuga Irena Katalinić Maja Vrdoljak Ana Lucić Rešić Arnes Karačonji Irena Brčić The effects of ketamine on viability, primary DNA damage, and oxidative stress parameters in HepG2 and SH-SY5Y cells Arhiv za Higijenu Rada i Toksikologiju anaesthetic antioxidant enzymes drug abuse ros toxicity anestezija antioksidacijski enzimi ros toksičnost zlouporaba droga |
| title | The effects of ketamine on viability, primary DNA damage, and oxidative stress parameters in HepG2 and SH-SY5Y cells |
| title_full | The effects of ketamine on viability, primary DNA damage, and oxidative stress parameters in HepG2 and SH-SY5Y cells |
| title_fullStr | The effects of ketamine on viability, primary DNA damage, and oxidative stress parameters in HepG2 and SH-SY5Y cells |
| title_full_unstemmed | The effects of ketamine on viability, primary DNA damage, and oxidative stress parameters in HepG2 and SH-SY5Y cells |
| title_short | The effects of ketamine on viability, primary DNA damage, and oxidative stress parameters in HepG2 and SH-SY5Y cells |
| title_sort | effects of ketamine on viability primary dna damage and oxidative stress parameters in hepg2 and sh sy5y cells |
| topic | anaesthetic antioxidant enzymes drug abuse ros toxicity anestezija antioksidacijski enzimi ros toksičnost zlouporaba droga |
| url | https://doi.org/10.2478/aiht-2023-74-3727 |
| work_keys_str_mv | AT juricandreja theeffectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT lovakovicblankatariba theeffectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT zandonaantonio theeffectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT rasicdubravka theeffectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT cesimartin theeffectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT pizentalica theeffectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT neubergmarijana theeffectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT canjugairena theeffectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT katalinicmaja theeffectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT vrdoljakanalucic theeffectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT resicarnes theeffectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT karaconjiirenabrcic theeffectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT juricandreja effectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT lovakovicblankatariba effectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT zandonaantonio effectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT rasicdubravka effectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT cesimartin effectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT pizentalica effectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT neubergmarijana effectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT canjugairena effectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT katalinicmaja effectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT vrdoljakanalucic effectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT resicarnes effectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells AT karaconjiirenabrcic effectsofketamineonviabilityprimarydnadamageandoxidativestressparametersinhepg2andshsy5ycells |