INTERLEUKIN 1A GENE POLYMORPHISMS IN ROMANIAN PATIENTS WITH SERONEGATIVE SPONDYLOARTHROPATHIES
Objective. The aim of this study was to investigate whether two IL1A gene polymorphisms, rs17561 and rs1800587, influence disease susceptibility for seronegative spondyloarthropathies (SpA) in Romanians. Subsequently, we analysed separately ankylosing spondylitis (AS) and psoriatic arthritis (PsA) s...
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| Format: | Article |
| Language: | English |
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Amaltea Medical Publishing House
2016-03-01
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| Series: | Romanian Journal of Rheumatology |
| Subjects: | |
| Online Access: | https://rjr.com.ro/articles/2016.1/RJR_2016_1_Art-03.pdf |
| _version_ | 1819239989538455552 |
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| author | Laura Ioana Cherciu Marius Cherciu Luis Ovidiu Popa Mihai Bojinca Monica Irina Dutescu Violeta Bojinca Constantin Bara Olivia Mihaela Popa |
| author_facet | Laura Ioana Cherciu Marius Cherciu Luis Ovidiu Popa Mihai Bojinca Monica Irina Dutescu Violeta Bojinca Constantin Bara Olivia Mihaela Popa |
| author_sort | Laura Ioana Cherciu |
| collection | DOAJ |
| description | Objective. The aim of this study was to investigate whether two IL1A gene polymorphisms, rs17561 and rs1800587, influence disease susceptibility for seronegative spondyloarthropathies (SpA) in Romanians. Subsequently, we analysed separately ankylosing spondylitis (AS) and psoriatic arthritis (PsA) subgroups in relation with the two IL1A gene variants.
Methods. The study included 240 SpA patients (140 AS and 100 PsA patients) and 160 healthy controls. Real-time polymerase chain reaction (RT-PCR) was used to genotype the two single nucleotide polymorphisms (SNPs). Allele, genotype and haplotype frequencies of each SNP were compared between SpA patients and controls and also between AS and PsA cohorts and controls. The PLINK 1.9 software package was used to assess the potential associations; p values ≤ 0.05 were considered significant.
Results. The minor allele T frequency for rs17561 polymorphism was similar in general SpA cohort (31.4%) compared with controls (32.8%), the statistical analyses confirming the lack of association (p=0.67). Almost identical results were found for rs1800587: minor allele T frequency was 29.1% in SpA patients and 29.7% in controls (p=0.85). The same pattern persisted for the separate analysis of HLA-B27 positive SpA patients or AS and PsA cases against controls.
Conclusions. The IL1A gene polymorphisms (rs17561 and rs1800587) do not influence the disease predisposition for seronegative spondyloarthropathies in general, nor for ankylosing spondylitis and psoriatic arthritis in
particular, in Romanians. |
| first_indexed | 2024-12-23T14:00:53Z |
| format | Article |
| id | doaj.art-90704fc3cbf34340a4221a12a5cb4a49 |
| institution | Directory Open Access Journal |
| issn | 1843-0791 2069-6086 |
| language | English |
| last_indexed | 2024-12-23T14:00:53Z |
| publishDate | 2016-03-01 |
| publisher | Amaltea Medical Publishing House |
| record_format | Article |
| series | Romanian Journal of Rheumatology |
| spelling | doaj.art-90704fc3cbf34340a4221a12a5cb4a492022-12-21T17:44:19ZengAmaltea Medical Publishing HouseRomanian Journal of Rheumatology1843-07912069-60862016-03-01251202610.37897/RJR.2016.1.3INTERLEUKIN 1A GENE POLYMORPHISMS IN ROMANIAN PATIENTS WITH SERONEGATIVE SPONDYLOARTHROPATHIESLaura Ioana Cherciu0Marius Cherciu1Luis Ovidiu Popa2Mihai Bojinca3Monica Irina Dutescu4Violeta Bojinca5Constantin Bara6Olivia Mihaela Popa7Department of Immunology and Pathophysiology, Faculty of Medicine, Carol Davila University, Bucharest, RomaniaDepartment of Immunology and Pathophysiology, Faculty of Medicine, Carol Davila University, Bucharest, RomaniaMolecular Biology Department, Grigore Antipa National Museum of Natural History, Bucharest, RomaniaDepartment of Rheumatology, Faculty of Medicine, Carol Davila University, Dr. Ion Cantacuzino Hospital, Bucharest, RomaniaProf. Dr. C.T. Nicolau National Institute of Blood Transfusion, Bucharest, RomaniaDepartment of Rheumatology, Faculty of Medicine, Carol Davila University, Sf. Maria Hospital Bucharest, RomaniaDepartment of Immunology and Pathophysiology, Faculty of Medicine, Carol Davila University, Bucharest, RomaniaDepartment of Immunology and Pathophysiology, Faculty of Medicine, Carol Davila University, Bucharest, RomaniaObjective. The aim of this study was to investigate whether two IL1A gene polymorphisms, rs17561 and rs1800587, influence disease susceptibility for seronegative spondyloarthropathies (SpA) in Romanians. Subsequently, we analysed separately ankylosing spondylitis (AS) and psoriatic arthritis (PsA) subgroups in relation with the two IL1A gene variants. Methods. The study included 240 SpA patients (140 AS and 100 PsA patients) and 160 healthy controls. Real-time polymerase chain reaction (RT-PCR) was used to genotype the two single nucleotide polymorphisms (SNPs). Allele, genotype and haplotype frequencies of each SNP were compared between SpA patients and controls and also between AS and PsA cohorts and controls. The PLINK 1.9 software package was used to assess the potential associations; p values ≤ 0.05 were considered significant. Results. The minor allele T frequency for rs17561 polymorphism was similar in general SpA cohort (31.4%) compared with controls (32.8%), the statistical analyses confirming the lack of association (p=0.67). Almost identical results were found for rs1800587: minor allele T frequency was 29.1% in SpA patients and 29.7% in controls (p=0.85). The same pattern persisted for the separate analysis of HLA-B27 positive SpA patients or AS and PsA cases against controls. Conclusions. The IL1A gene polymorphisms (rs17561 and rs1800587) do not influence the disease predisposition for seronegative spondyloarthropathies in general, nor for ankylosing spondylitis and psoriatic arthritis in particular, in Romanians.https://rjr.com.ro/articles/2016.1/RJR_2016_1_Art-03.pdfil1a geneseronegative spondyloarthropathies (spa)ankylosing spondylitis (as)psoriatic arthritis (psa)single nucleotide polymorphisms (snps) |
| spellingShingle | Laura Ioana Cherciu Marius Cherciu Luis Ovidiu Popa Mihai Bojinca Monica Irina Dutescu Violeta Bojinca Constantin Bara Olivia Mihaela Popa INTERLEUKIN 1A GENE POLYMORPHISMS IN ROMANIAN PATIENTS WITH SERONEGATIVE SPONDYLOARTHROPATHIES Romanian Journal of Rheumatology il1a gene seronegative spondyloarthropathies (spa) ankylosing spondylitis (as) psoriatic arthritis (psa) single nucleotide polymorphisms (snps) |
| title | INTERLEUKIN 1A GENE POLYMORPHISMS IN ROMANIAN PATIENTS WITH SERONEGATIVE SPONDYLOARTHROPATHIES |
| title_full | INTERLEUKIN 1A GENE POLYMORPHISMS IN ROMANIAN PATIENTS WITH SERONEGATIVE SPONDYLOARTHROPATHIES |
| title_fullStr | INTERLEUKIN 1A GENE POLYMORPHISMS IN ROMANIAN PATIENTS WITH SERONEGATIVE SPONDYLOARTHROPATHIES |
| title_full_unstemmed | INTERLEUKIN 1A GENE POLYMORPHISMS IN ROMANIAN PATIENTS WITH SERONEGATIVE SPONDYLOARTHROPATHIES |
| title_short | INTERLEUKIN 1A GENE POLYMORPHISMS IN ROMANIAN PATIENTS WITH SERONEGATIVE SPONDYLOARTHROPATHIES |
| title_sort | interleukin 1a gene polymorphisms in romanian patients with seronegative spondyloarthropathies |
| topic | il1a gene seronegative spondyloarthropathies (spa) ankylosing spondylitis (as) psoriatic arthritis (psa) single nucleotide polymorphisms (snps) |
| url | https://rjr.com.ro/articles/2016.1/RJR_2016_1_Art-03.pdf |
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