Latissimus Dorsi Myocutaneous Flap Procedure in a Swine Model
Background As surgical research expands in both breadth and scope, translational models become increasingly important. The accessibility, reproducibility, and clinical applicability of translational models is of vital importance to ensure adequate and accurate research. Though different flap models...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-11-01
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| Series: | Journal of Investigative Surgery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08941939.2020.1795952 |
| _version_ | 1797684550085640192 |
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| author | Joanna W. Etra Samuel A. J. Fidder Christopher M. Frost Franka Messner Yinan Guo Dalibor Vasilic Sarah E. Beck Steven Bonawitz Gerald Brandacher Damon S. Cooney |
| author_facet | Joanna W. Etra Samuel A. J. Fidder Christopher M. Frost Franka Messner Yinan Guo Dalibor Vasilic Sarah E. Beck Steven Bonawitz Gerald Brandacher Damon S. Cooney |
| author_sort | Joanna W. Etra |
| collection | DOAJ |
| description | Background As surgical research expands in both breadth and scope, translational models become increasingly important. The accessibility, reproducibility, and clinical applicability of translational models is of vital importance to ensure adequate and accurate research. Though different flap models have been described, the literature lacks an in-depth, technical description of an easy large-animal preclinical model. We here describe the procedure for elevation of a latissimus dorsi flap in a swine. This flap contains muscle and skin that can be isolated on a vascular pedicle, transferred as a free flap, perfused, or innervated/denervated as dictated by the needs of the experiment. Methods Five different latissimus dorsi flaps were elevated in miniature swine. Careful attention was paid to anatomical landmarks and optimal placement of incision, dissection, and retraction. Temporary ischemia with vascular clamping was performed along with serial digital and infrared imaging both intra- and postoperatively. In three of the flaps with induced ischemia, the animal was observed for a 30-day follow up with daily photodocumentation and intermittent biopsy. Results A reproducible latissimus flap model was designed with optimized conditions. In the animals in which flaps were followed postoperatively, complete healing was seen within 30 days without evidence of procedure-related ischemia or loss of motor function. Conclusion We have identified and described a pre-clinical large animal flap model that can be easily reproduced for translational studies of multiple scientific areas including flap-based repair, ischemia, ischemia reperfusion, and operative technique. This provides an important model for ready replication in preclinical studies of many varieties. |
| first_indexed | 2024-03-12T00:31:23Z |
| format | Article |
| id | doaj.art-90708c3712694ab3ac23b476bec14500 |
| institution | Directory Open Access Journal |
| issn | 0894-1939 1521-0553 |
| language | English |
| last_indexed | 2024-03-12T00:31:23Z |
| publishDate | 2021-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Investigative Surgery |
| spelling | doaj.art-90708c3712694ab3ac23b476bec145002023-09-15T10:12:29ZengTaylor & Francis GroupJournal of Investigative Surgery0894-19391521-05532021-11-0134121289129610.1080/08941939.2020.17959521795952Latissimus Dorsi Myocutaneous Flap Procedure in a Swine ModelJoanna W. Etra0Samuel A. J. Fidder1Christopher M. Frost2Franka Messner3Yinan Guo4Dalibor Vasilic5Sarah E. Beck6Steven Bonawitz7Gerald Brandacher8Damon S. Cooney9Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, School of Medicine, Johns Hopkins UniversityDepartment of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, School of Medicine, Johns Hopkins UniversityDepartment of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, School of Medicine, Johns Hopkins UniversityDepartment of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, School of Medicine, Johns Hopkins UniversityDepartment of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, School of Medicine, Johns Hopkins UniversityDepartment of Plastic and Reconstructive Surgery, Erasmus University Medical CenterDepartment of Molecular and Comparative Pathobiology, School of Medicine, Johns Hopkins UniversityDepartment of Surgery, Cooper Medical School, Rowan UniversityDepartment of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, School of Medicine, Johns Hopkins UniversityDepartment of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, School of Medicine, Johns Hopkins UniversityBackground As surgical research expands in both breadth and scope, translational models become increasingly important. The accessibility, reproducibility, and clinical applicability of translational models is of vital importance to ensure adequate and accurate research. Though different flap models have been described, the literature lacks an in-depth, technical description of an easy large-animal preclinical model. We here describe the procedure for elevation of a latissimus dorsi flap in a swine. This flap contains muscle and skin that can be isolated on a vascular pedicle, transferred as a free flap, perfused, or innervated/denervated as dictated by the needs of the experiment. Methods Five different latissimus dorsi flaps were elevated in miniature swine. Careful attention was paid to anatomical landmarks and optimal placement of incision, dissection, and retraction. Temporary ischemia with vascular clamping was performed along with serial digital and infrared imaging both intra- and postoperatively. In three of the flaps with induced ischemia, the animal was observed for a 30-day follow up with daily photodocumentation and intermittent biopsy. Results A reproducible latissimus flap model was designed with optimized conditions. In the animals in which flaps were followed postoperatively, complete healing was seen within 30 days without evidence of procedure-related ischemia or loss of motor function. Conclusion We have identified and described a pre-clinical large animal flap model that can be easily reproduced for translational studies of multiple scientific areas including flap-based repair, ischemia, ischemia reperfusion, and operative technique. This provides an important model for ready replication in preclinical studies of many varieties.http://dx.doi.org/10.1080/08941939.2020.1795952latissimus flapmyocutaneous flapswine flapswine latissimus |
| spellingShingle | Joanna W. Etra Samuel A. J. Fidder Christopher M. Frost Franka Messner Yinan Guo Dalibor Vasilic Sarah E. Beck Steven Bonawitz Gerald Brandacher Damon S. Cooney Latissimus Dorsi Myocutaneous Flap Procedure in a Swine Model Journal of Investigative Surgery latissimus flap myocutaneous flap swine flap swine latissimus |
| title | Latissimus Dorsi Myocutaneous Flap Procedure in a Swine Model |
| title_full | Latissimus Dorsi Myocutaneous Flap Procedure in a Swine Model |
| title_fullStr | Latissimus Dorsi Myocutaneous Flap Procedure in a Swine Model |
| title_full_unstemmed | Latissimus Dorsi Myocutaneous Flap Procedure in a Swine Model |
| title_short | Latissimus Dorsi Myocutaneous Flap Procedure in a Swine Model |
| title_sort | latissimus dorsi myocutaneous flap procedure in a swine model |
| topic | latissimus flap myocutaneous flap swine flap swine latissimus |
| url | http://dx.doi.org/10.1080/08941939.2020.1795952 |
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