Purification and characterisation of a fungicidal peptide from Bacillus amyloliquefaciens NCPSJ7
Bacillus amyloliquefaciens NCPSJ7 could secrete extracellular antimicrobial substances, showing potent antifungal activities. An active peptide AFP3 was isolated from the fermentation supernatant. After chromatography, the purified peptide was tested for the fungicidal activity, molecular mass, and...
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Czech Academy of Agricultural Sciences
2017-04-01
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| Series: | Czech Journal of Food Sciences |
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| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201702-0003_purification-and-characterisation-of-a-fungicidal-peptide-from-bacillus-amyloliquefaciens-ncpsj7.php |
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| author | Junhua Wang Shuangzhi Zhao Jiying Qiu Qingxin Zhou Xiaoyong Liu Xue Xin Danyang Guo Tatyana G. Yudina Yifen Wang$ Hua Sun Xiangyan Chen Leilei Chen |
| author_facet | Junhua Wang Shuangzhi Zhao Jiying Qiu Qingxin Zhou Xiaoyong Liu Xue Xin Danyang Guo Tatyana G. Yudina Yifen Wang$ Hua Sun Xiangyan Chen Leilei Chen |
| author_sort | Junhua Wang |
| collection | DOAJ |
| description | Bacillus amyloliquefaciens NCPSJ7 could secrete extracellular antimicrobial substances, showing potent antifungal activities. An active peptide AFP3 was isolated from the fermentation supernatant. After chromatography, the purified peptide was tested for the fungicidal activity, molecular mass, and stability. The results indicated that the peptide with a molecular mass of around 3.3 kDa, showed discernible inhibition of the pathogen Fusarium oxysporum f. sp. niveum with the minimum fungicidal concentration of 31 µg/ml. It also exhibited excellent inhibition of some representative pathogenic fungi at a low concentration. Moreover, the peptide remained active at a wide range of temperatures and pH. Ion Na+ may even increase the antifungal activities. At the same time, the peptide could well tolerate the treatment with trypsin. Electron microscopy was used to investigate the effect of the peptide on the pathogens. The peptide inhibited the growth of pathogens by disrupting the integrity of the hyphal membranes, resulting in their lysis. The potent fungicidal activities and stability made the peptide be a candidate for a biopreservative. |
| first_indexed | 2024-04-10T08:32:52Z |
| format | Article |
| id | doaj.art-90714cac5ed74574b8a3e5b944228146 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:32:52Z |
| publishDate | 2017-04-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-90714cac5ed74574b8a3e5b9442281462023-02-23T03:28:25ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172017-04-0135211312110.17221/163/2016-CJFScjf-201702-0003Purification and characterisation of a fungicidal peptide from Bacillus amyloliquefaciens NCPSJ7Junhua Wang0Shuangzhi Zhao1Jiying Qiu2Qingxin Zhou3Xiaoyong Liu4Xue Xin5Danyang Guo6Tatyana G. Yudina7Yifen Wang$8Hua Sun9Xiangyan Chen10Leilei Chen11Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. ChinaInstitute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. ChinaInstitute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. ChinaInstitute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. ChinaInstitute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. ChinaInstitute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. ChinaDepartment of Microbiology, Moscow State University, Moscow, RussiaDepartment of Microbiology, Moscow State University, Moscow, RussiaInstitute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. ChinaInstitute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. ChinaInstitute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. ChinaInstitute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. ChinaBacillus amyloliquefaciens NCPSJ7 could secrete extracellular antimicrobial substances, showing potent antifungal activities. An active peptide AFP3 was isolated from the fermentation supernatant. After chromatography, the purified peptide was tested for the fungicidal activity, molecular mass, and stability. The results indicated that the peptide with a molecular mass of around 3.3 kDa, showed discernible inhibition of the pathogen Fusarium oxysporum f. sp. niveum with the minimum fungicidal concentration of 31 µg/ml. It also exhibited excellent inhibition of some representative pathogenic fungi at a low concentration. Moreover, the peptide remained active at a wide range of temperatures and pH. Ion Na+ may even increase the antifungal activities. At the same time, the peptide could well tolerate the treatment with trypsin. Electron microscopy was used to investigate the effect of the peptide on the pathogens. The peptide inhibited the growth of pathogens by disrupting the integrity of the hyphal membranes, resulting in their lysis. The potent fungicidal activities and stability made the peptide be a candidate for a biopreservative.https://cjfs.agriculturejournals.cz/artkey/cjf-201702-0003_purification-and-characterisation-of-a-fungicidal-peptide-from-bacillus-amyloliquefaciens-ncpsj7.phpantifungal peptidemechanismstabilitybiopreservative |
| spellingShingle | Junhua Wang Shuangzhi Zhao Jiying Qiu Qingxin Zhou Xiaoyong Liu Xue Xin Danyang Guo Tatyana G. Yudina Yifen Wang$ Hua Sun Xiangyan Chen Leilei Chen Purification and characterisation of a fungicidal peptide from Bacillus amyloliquefaciens NCPSJ7 Czech Journal of Food Sciences antifungal peptide mechanism stability biopreservative |
| title | Purification and characterisation of a fungicidal peptide from Bacillus amyloliquefaciens NCPSJ7 |
| title_full | Purification and characterisation of a fungicidal peptide from Bacillus amyloliquefaciens NCPSJ7 |
| title_fullStr | Purification and characterisation of a fungicidal peptide from Bacillus amyloliquefaciens NCPSJ7 |
| title_full_unstemmed | Purification and characterisation of a fungicidal peptide from Bacillus amyloliquefaciens NCPSJ7 |
| title_short | Purification and characterisation of a fungicidal peptide from Bacillus amyloliquefaciens NCPSJ7 |
| title_sort | purification and characterisation of a fungicidal peptide from bacillus amyloliquefaciens ncpsj7 |
| topic | antifungal peptide mechanism stability biopreservative |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201702-0003_purification-and-characterisation-of-a-fungicidal-peptide-from-bacillus-amyloliquefaciens-ncpsj7.php |
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