The connecting cilium inner scaffold provides a structural foundation that protects against retinal degeneration
Inherited retinal degeneration due to loss of photoreceptor cells is a leading cause of human blindness. These cells possess a photosensitive outer segment linked to the cell body through the connecting cilium (CC). While structural defects of the CC have been associated with retinal degeneration, i...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-06-01
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| Series: | PLoS Biology |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202906/?tool=EBI |
| _version_ | 1818156856493735936 |
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| author | Olivier Mercey Corinne Kostic Eloïse Bertiaux Alexia Giroud Yashar Sadian David C. A. Gaboriau Ciaran G. Morrison Ning Chang Yvan Arsenijevic Paul Guichard Virginie Hamel |
| author_facet | Olivier Mercey Corinne Kostic Eloïse Bertiaux Alexia Giroud Yashar Sadian David C. A. Gaboriau Ciaran G. Morrison Ning Chang Yvan Arsenijevic Paul Guichard Virginie Hamel |
| author_sort | Olivier Mercey |
| collection | DOAJ |
| description | Inherited retinal degeneration due to loss of photoreceptor cells is a leading cause of human blindness. These cells possess a photosensitive outer segment linked to the cell body through the connecting cilium (CC). While structural defects of the CC have been associated with retinal degeneration, its nanoscale molecular composition, assembly, and function are barely known. Here, using expansion microscopy and electron microscopy, we reveal the molecular architecture of the CC and demonstrate that microtubules are linked together by a CC inner scaffold containing POC5, CENTRIN, and FAM161A. Dissecting CC inner scaffold assembly during photoreceptor development in mouse revealed that it acts as a structural zipper, progressively bridging microtubule doublets and straightening the CC. Furthermore, we show that Fam161a disruption in mouse leads to specific CC inner scaffold loss and triggers microtubule doublet spreading, prior to outer segment collapse and photoreceptor degeneration, suggesting a molecular mechanism for a subtype of retinitis pigmentosa. Inherited retinal degeneration due to loss of photoreceptor cells is a leading cause of human blindness. Ultrastructure expansion microscopy on mouse retina reveals the presence of a novel structure inside the photoreceptor connecting cilium, the inner scaffold, that protects the outer segment against degeneration. |
| first_indexed | 2024-12-11T15:04:57Z |
| format | Article |
| id | doaj.art-90718703dd5f45bcb73af6fa8af31971 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-11T15:04:57Z |
| publishDate | 2022-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-90718703dd5f45bcb73af6fa8af319712022-12-22T01:00:59ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852022-06-01206The connecting cilium inner scaffold provides a structural foundation that protects against retinal degenerationOlivier MerceyCorinne KosticEloïse BertiauxAlexia GiroudYashar SadianDavid C. A. GaboriauCiaran G. MorrisonNing ChangYvan ArsenijevicPaul GuichardVirginie HamelInherited retinal degeneration due to loss of photoreceptor cells is a leading cause of human blindness. These cells possess a photosensitive outer segment linked to the cell body through the connecting cilium (CC). While structural defects of the CC have been associated with retinal degeneration, its nanoscale molecular composition, assembly, and function are barely known. Here, using expansion microscopy and electron microscopy, we reveal the molecular architecture of the CC and demonstrate that microtubules are linked together by a CC inner scaffold containing POC5, CENTRIN, and FAM161A. Dissecting CC inner scaffold assembly during photoreceptor development in mouse revealed that it acts as a structural zipper, progressively bridging microtubule doublets and straightening the CC. Furthermore, we show that Fam161a disruption in mouse leads to specific CC inner scaffold loss and triggers microtubule doublet spreading, prior to outer segment collapse and photoreceptor degeneration, suggesting a molecular mechanism for a subtype of retinitis pigmentosa. Inherited retinal degeneration due to loss of photoreceptor cells is a leading cause of human blindness. Ultrastructure expansion microscopy on mouse retina reveals the presence of a novel structure inside the photoreceptor connecting cilium, the inner scaffold, that protects the outer segment against degeneration.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202906/?tool=EBI |
| spellingShingle | Olivier Mercey Corinne Kostic Eloïse Bertiaux Alexia Giroud Yashar Sadian David C. A. Gaboriau Ciaran G. Morrison Ning Chang Yvan Arsenijevic Paul Guichard Virginie Hamel The connecting cilium inner scaffold provides a structural foundation that protects against retinal degeneration PLoS Biology |
| title | The connecting cilium inner scaffold provides a structural foundation that protects against retinal degeneration |
| title_full | The connecting cilium inner scaffold provides a structural foundation that protects against retinal degeneration |
| title_fullStr | The connecting cilium inner scaffold provides a structural foundation that protects against retinal degeneration |
| title_full_unstemmed | The connecting cilium inner scaffold provides a structural foundation that protects against retinal degeneration |
| title_short | The connecting cilium inner scaffold provides a structural foundation that protects against retinal degeneration |
| title_sort | connecting cilium inner scaffold provides a structural foundation that protects against retinal degeneration |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202906/?tool=EBI |
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