Colonization of Serendipita indica promotes resistance against Spodoptera exigua in onion (Allium cepa L.)
Plant-endophyte symbiosis influences plant defense and growth. Serendipita indica is a root endophyte that promotes growth and induces tolerance against biotic and abiotic stress in plants. In this study, we examined the effect of S. indica colonization on herbivore (Spodoptera exigua) resistance of...
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| Format: | Article |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1190942/full |
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| author | Praveen Roylawar Praveen Roylawar Kiran Khandagale Satyabrata Nanda Parakkattu Sulochanan Soumia Sangita Jadhav Vijay Mahajan Suresh Gawande |
| author_facet | Praveen Roylawar Praveen Roylawar Kiran Khandagale Satyabrata Nanda Parakkattu Sulochanan Soumia Sangita Jadhav Vijay Mahajan Suresh Gawande |
| author_sort | Praveen Roylawar |
| collection | DOAJ |
| description | Plant-endophyte symbiosis influences plant defense and growth. Serendipita indica is a root endophyte that promotes growth and induces tolerance against biotic and abiotic stress in plants. In this study, we examined the effect of S. indica colonization on herbivore (Spodoptera exigua) resistance of onion (Allium cepa L.). We found that colonization of S. indica in the roots of onion significantly reduced the feeding damage of leaves by S. exigua larvae, and also resulted in a reduction in weight gain of the larvae when fed on S. indica plants. This enhanced resistance is a result of modulation of antioxidant and defense enzymes/genes in the host by S. indica mutualism. Specifically, the activities of enzymes such as Superoxide dismutase, peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase, and H2O2 content were significantly higher in the early stages of S. exigua feeding in the S. indica colonized plants compared to the non-colonized counterparts. Similarly, defense genes also showed modulation in response to this tripartite interaction of onion -S. indica mutualism and S. exigua herbivory. The hierarchical cluster analysis and principal component analysis indicated a clear difference in the onion biochemical responses, which is due to the S. indica symbiosis. Our investigation demonstrates that onion-S. indica symbiosis significantly decreases chewing injury by efficiently modulating antioxidant and defense enzyme activities and gene expression in response to S. exigua herbivory. Therefore, S. indica can be used as a potential biocontrol agent for sustainable management of this important pest of Alliums. |
| first_indexed | 2024-03-12T21:47:24Z |
| format | Article |
| id | doaj.art-90722a022b13485d9d36ebcfe45122bf |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T21:47:24Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-90722a022b13485d9d36ebcfe45122bf2023-07-26T09:43:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-07-011410.3389/fmicb.2023.11909421190942Colonization of Serendipita indica promotes resistance against Spodoptera exigua in onion (Allium cepa L.)Praveen Roylawar0Praveen Roylawar1Kiran Khandagale2Satyabrata Nanda3Parakkattu Sulochanan Soumia4Sangita Jadhav5Vijay Mahajan6Suresh Gawande7ICAR-Directorate of Onion and Garlic Research, Pune, IndiaDepartment of Botany, S.N. Arts, D.J.M. Commerce and B.N.S. Science College, Sangamner, Maharashtra, IndiaICAR-Directorate of Onion and Garlic Research, Pune, IndiaDepartment of Biotechnology, Centurion University of Technology and Management, Paralakhemundi, IndiaICAR-Directorate of Onion and Garlic Research, Pune, IndiaDepartment of Botany, S.N. Arts, D.J.M. Commerce and B.N.S. Science College, Sangamner, Maharashtra, IndiaICAR-Directorate of Onion and Garlic Research, Pune, IndiaICAR-Directorate of Onion and Garlic Research, Pune, IndiaPlant-endophyte symbiosis influences plant defense and growth. Serendipita indica is a root endophyte that promotes growth and induces tolerance against biotic and abiotic stress in plants. In this study, we examined the effect of S. indica colonization on herbivore (Spodoptera exigua) resistance of onion (Allium cepa L.). We found that colonization of S. indica in the roots of onion significantly reduced the feeding damage of leaves by S. exigua larvae, and also resulted in a reduction in weight gain of the larvae when fed on S. indica plants. This enhanced resistance is a result of modulation of antioxidant and defense enzymes/genes in the host by S. indica mutualism. Specifically, the activities of enzymes such as Superoxide dismutase, peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase, and H2O2 content were significantly higher in the early stages of S. exigua feeding in the S. indica colonized plants compared to the non-colonized counterparts. Similarly, defense genes also showed modulation in response to this tripartite interaction of onion -S. indica mutualism and S. exigua herbivory. The hierarchical cluster analysis and principal component analysis indicated a clear difference in the onion biochemical responses, which is due to the S. indica symbiosis. Our investigation demonstrates that onion-S. indica symbiosis significantly decreases chewing injury by efficiently modulating antioxidant and defense enzyme activities and gene expression in response to S. exigua herbivory. Therefore, S. indica can be used as a potential biocontrol agent for sustainable management of this important pest of Alliums.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1190942/fullonionSpodoptera exiguaSerendipita indicainsect herbivoryendophyte-mediated resistance |
| spellingShingle | Praveen Roylawar Praveen Roylawar Kiran Khandagale Satyabrata Nanda Parakkattu Sulochanan Soumia Sangita Jadhav Vijay Mahajan Suresh Gawande Colonization of Serendipita indica promotes resistance against Spodoptera exigua in onion (Allium cepa L.) Frontiers in Microbiology onion Spodoptera exigua Serendipita indica insect herbivory endophyte-mediated resistance |
| title | Colonization of Serendipita indica promotes resistance against Spodoptera exigua in onion (Allium cepa L.) |
| title_full | Colonization of Serendipita indica promotes resistance against Spodoptera exigua in onion (Allium cepa L.) |
| title_fullStr | Colonization of Serendipita indica promotes resistance against Spodoptera exigua in onion (Allium cepa L.) |
| title_full_unstemmed | Colonization of Serendipita indica promotes resistance against Spodoptera exigua in onion (Allium cepa L.) |
| title_short | Colonization of Serendipita indica promotes resistance against Spodoptera exigua in onion (Allium cepa L.) |
| title_sort | colonization of serendipita indica promotes resistance against spodoptera exigua in onion allium cepa l |
| topic | onion Spodoptera exigua Serendipita indica insect herbivory endophyte-mediated resistance |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1190942/full |
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