Proteomic Studies of the Biofilm Matrix including Outer Membrane Vesicles of <i>Burkholderia multivorans</i> C1576, a Strain of Clinical Importance for Cystic Fibrosis
Biofilms are aggregates of microbial cells encased in a highly hydrated matrix made up of self-produced extracellular polymeric substances (EPS) which consist of polysaccharides, proteins, nucleic acids, and lipids. While biofilm matrix polysaccharides are unraveled, there is still poor knowledge ab...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/11/1826 |
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| author | Lucrecia C. Terán Marco Distefano Barbara Bellich Sara Petrosino Paolo Bertoncin Paola Cescutti Daniele Sblattero |
| author_facet | Lucrecia C. Terán Marco Distefano Barbara Bellich Sara Petrosino Paolo Bertoncin Paola Cescutti Daniele Sblattero |
| author_sort | Lucrecia C. Terán |
| collection | DOAJ |
| description | Biofilms are aggregates of microbial cells encased in a highly hydrated matrix made up of self-produced extracellular polymeric substances (EPS) which consist of polysaccharides, proteins, nucleic acids, and lipids. While biofilm matrix polysaccharides are unraveled, there is still poor knowledge about the identity and function of matrix-associated proteins. With this work, we performed a comprehensive proteomic approach to disclose the identity of proteins associated with the matrix of biofilm-growing <i>Burkholderia multivorans</i> C1576 reference strain, a cystic fibrosis clinical isolate. Transmission electron microscopy showed that <i>B. multivorans</i> C1576 also releases outer membrane vesicles (OMVs) in the biofilm matrix, as already demonstrated for other Gram-negative species. The proteomic analysis revealed that cytoplasmic and membrane-bound proteins are widely represented in the matrix, while OMVs are highly enriched in outer membrane proteins and siderophores. Our data suggest that cell lysis and OMVs production are the most important sources of proteins for the <i>B. multivorans</i> C1576 biofilm matrix. Of note, some of the identified proteins are lytic enzymes, siderophores, and proteins involved in reactive oxygen species (ROS) scavenging. These proteins might help <i>B. multivorans</i> C1576 in host tissue invasion and defense towards immune system assaults. |
| first_indexed | 2024-03-10T14:44:00Z |
| format | Article |
| id | doaj.art-9074b9cdc1d849a98d15c36e73c2bcb9 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T14:44:00Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-9074b9cdc1d849a98d15c36e73c2bcb92023-11-20T21:33:47ZengMDPI AGMicroorganisms2076-26072020-11-01811182610.3390/microorganisms8111826Proteomic Studies of the Biofilm Matrix including Outer Membrane Vesicles of <i>Burkholderia multivorans</i> C1576, a Strain of Clinical Importance for Cystic FibrosisLucrecia C. Terán0Marco Distefano1Barbara Bellich2Sara Petrosino3Paolo Bertoncin4Paola Cescutti5Daniele Sblattero6Department of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyBiofilms are aggregates of microbial cells encased in a highly hydrated matrix made up of self-produced extracellular polymeric substances (EPS) which consist of polysaccharides, proteins, nucleic acids, and lipids. While biofilm matrix polysaccharides are unraveled, there is still poor knowledge about the identity and function of matrix-associated proteins. With this work, we performed a comprehensive proteomic approach to disclose the identity of proteins associated with the matrix of biofilm-growing <i>Burkholderia multivorans</i> C1576 reference strain, a cystic fibrosis clinical isolate. Transmission electron microscopy showed that <i>B. multivorans</i> C1576 also releases outer membrane vesicles (OMVs) in the biofilm matrix, as already demonstrated for other Gram-negative species. The proteomic analysis revealed that cytoplasmic and membrane-bound proteins are widely represented in the matrix, while OMVs are highly enriched in outer membrane proteins and siderophores. Our data suggest that cell lysis and OMVs production are the most important sources of proteins for the <i>B. multivorans</i> C1576 biofilm matrix. Of note, some of the identified proteins are lytic enzymes, siderophores, and proteins involved in reactive oxygen species (ROS) scavenging. These proteins might help <i>B. multivorans</i> C1576 in host tissue invasion and defense towards immune system assaults.https://www.mdpi.com/2076-2607/8/11/1826<i>Burkholderia multivorans</i>proteomicbiofilmouter membrane vesiclesLC-LC/MS |
| spellingShingle | Lucrecia C. Terán Marco Distefano Barbara Bellich Sara Petrosino Paolo Bertoncin Paola Cescutti Daniele Sblattero Proteomic Studies of the Biofilm Matrix including Outer Membrane Vesicles of <i>Burkholderia multivorans</i> C1576, a Strain of Clinical Importance for Cystic Fibrosis Microorganisms <i>Burkholderia multivorans</i> proteomic biofilm outer membrane vesicles LC-LC/MS |
| title | Proteomic Studies of the Biofilm Matrix including Outer Membrane Vesicles of <i>Burkholderia multivorans</i> C1576, a Strain of Clinical Importance for Cystic Fibrosis |
| title_full | Proteomic Studies of the Biofilm Matrix including Outer Membrane Vesicles of <i>Burkholderia multivorans</i> C1576, a Strain of Clinical Importance for Cystic Fibrosis |
| title_fullStr | Proteomic Studies of the Biofilm Matrix including Outer Membrane Vesicles of <i>Burkholderia multivorans</i> C1576, a Strain of Clinical Importance for Cystic Fibrosis |
| title_full_unstemmed | Proteomic Studies of the Biofilm Matrix including Outer Membrane Vesicles of <i>Burkholderia multivorans</i> C1576, a Strain of Clinical Importance for Cystic Fibrosis |
| title_short | Proteomic Studies of the Biofilm Matrix including Outer Membrane Vesicles of <i>Burkholderia multivorans</i> C1576, a Strain of Clinical Importance for Cystic Fibrosis |
| title_sort | proteomic studies of the biofilm matrix including outer membrane vesicles of i burkholderia multivorans i c1576 a strain of clinical importance for cystic fibrosis |
| topic | <i>Burkholderia multivorans</i> proteomic biofilm outer membrane vesicles LC-LC/MS |
| url | https://www.mdpi.com/2076-2607/8/11/1826 |
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