Detection of Salmonella pathogenicity island and Salmonella plasmid virulence genes in Salmonella Enteritidis originated from layer and broiler farms in Java Island
Objective: The incidence of salmonellosis in humans and animals is still high due to the occur¬rence of virulence factors in Salmonella enterica which play a role in the process of infection in the host and the spread of disease and most of the S. enterica can infect humans and animals. The present...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2019-09-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=51029 |
| _version_ | 1811269520441999360 |
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| author | Ernes Andesfha Agustin Indrawati Ni Luh Putu Ika Mayasari Irma Rahayuningtyas Istiyaningsih Jusa |
| author_facet | Ernes Andesfha Agustin Indrawati Ni Luh Putu Ika Mayasari Irma Rahayuningtyas Istiyaningsih Jusa |
| author_sort | Ernes Andesfha |
| collection | DOAJ |
| description | Objective: The incidence of salmonellosis in humans and animals is still high due to the occur¬rence of virulence factors in Salmonella enterica which play a role in the process of infection in the host and the spread of disease and most of the S. enterica can infect humans and animals. The present study was aimed to identify Salmonella Enteritidis and detect virulence genes related to Salmonella pathogenicity islands (SPIs) and Salmonella plasmid virulence (Spv).
Materials and Methods: A total of 27 S. Enteritidis archive isolates belonging to the National Veterinary Drug Assay Laboratory (NVDAL) were used in this study. The bacteria were collected in 2016 and 2017 from samples of the cloaca and fecal swabs from layer and broiler farms in five provinces of Java Island. Isolates were cultured in specific media, biochemical tests and Gram staining. Detection of S. Enteritidis and virulence genes was done by polymerase chain reaction (PCR) method.
Results: Identification of serovar showed 100% (27/27) isolates were positive for the sdfI gene (304 bp). The result confirmed that all strains were S. Enteritidis. PCR based detection of virulence genes showed that 100% of isolates had virulence genes in SPI-1 to SPI-5, namely, invA, ssaQ, mgtC, spi4D, and pipA genes. All the isolates (27/27) were also positive to spvB gene-based PCR.
Conclusion: All the isolates of S. Enteritidis in this study carry virulence genes related to SPI-1 to SPI-5 and plasmid virulence. The existence of virulent genes indicates that the S. Enteritidis strain examined in this study is highly virulent and poses a potential threat of worse disease outcome in humans and animals. [J Adv Vet Anim Res 2019; 6(3.000): 384-393] |
| first_indexed | 2024-04-12T21:43:32Z |
| format | Article |
| id | doaj.art-90769416db624e21beffd8794aed1438 |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-04-12T21:43:32Z |
| publishDate | 2019-09-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-90769416db624e21beffd8794aed14382022-12-22T03:15:43ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102019-09-016338439310.5455/javar.2019.f35851029Detection of Salmonella pathogenicity island and Salmonella plasmid virulence genes in Salmonella Enteritidis originated from layer and broiler farms in Java IslandErnes Andesfha0Agustin Indrawati1Ni Luh Putu Ika Mayasari2Irma Rahayuningtyas3Istiyaningsih Jusa4Department of Animal Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia Department of Animal Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia Department of Animal Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia National Veterinary Drug Assay Laboratory NVDAL, Bogor, Indonesia National Veterinary Drug Assay Laboratory NVDAL, Bogor, Indonesia.Objective: The incidence of salmonellosis in humans and animals is still high due to the occur¬rence of virulence factors in Salmonella enterica which play a role in the process of infection in the host and the spread of disease and most of the S. enterica can infect humans and animals. The present study was aimed to identify Salmonella Enteritidis and detect virulence genes related to Salmonella pathogenicity islands (SPIs) and Salmonella plasmid virulence (Spv). Materials and Methods: A total of 27 S. Enteritidis archive isolates belonging to the National Veterinary Drug Assay Laboratory (NVDAL) were used in this study. The bacteria were collected in 2016 and 2017 from samples of the cloaca and fecal swabs from layer and broiler farms in five provinces of Java Island. Isolates were cultured in specific media, biochemical tests and Gram staining. Detection of S. Enteritidis and virulence genes was done by polymerase chain reaction (PCR) method. Results: Identification of serovar showed 100% (27/27) isolates were positive for the sdfI gene (304 bp). The result confirmed that all strains were S. Enteritidis. PCR based detection of virulence genes showed that 100% of isolates had virulence genes in SPI-1 to SPI-5, namely, invA, ssaQ, mgtC, spi4D, and pipA genes. All the isolates (27/27) were also positive to spvB gene-based PCR. Conclusion: All the isolates of S. Enteritidis in this study carry virulence genes related to SPI-1 to SPI-5 and plasmid virulence. The existence of virulent genes indicates that the S. Enteritidis strain examined in this study is highly virulent and poses a potential threat of worse disease outcome in humans and animals. [J Adv Vet Anim Res 2019; 6(3.000): 384-393]http://www.ejmanager.com/fulltextpdf.php?mno=51029Java Island; S. Enteritidis; virulence genes; SPIs; virulence plasmids |
| spellingShingle | Ernes Andesfha Agustin Indrawati Ni Luh Putu Ika Mayasari Irma Rahayuningtyas Istiyaningsih Jusa Detection of Salmonella pathogenicity island and Salmonella plasmid virulence genes in Salmonella Enteritidis originated from layer and broiler farms in Java Island Journal of Advanced Veterinary and Animal Research Java Island; S. Enteritidis; virulence genes; SPIs; virulence plasmids |
| title | Detection of Salmonella pathogenicity island and Salmonella plasmid virulence genes in Salmonella Enteritidis originated from layer and broiler farms in Java Island |
| title_full | Detection of Salmonella pathogenicity island and Salmonella plasmid virulence genes in Salmonella Enteritidis originated from layer and broiler farms in Java Island |
| title_fullStr | Detection of Salmonella pathogenicity island and Salmonella plasmid virulence genes in Salmonella Enteritidis originated from layer and broiler farms in Java Island |
| title_full_unstemmed | Detection of Salmonella pathogenicity island and Salmonella plasmid virulence genes in Salmonella Enteritidis originated from layer and broiler farms in Java Island |
| title_short | Detection of Salmonella pathogenicity island and Salmonella plasmid virulence genes in Salmonella Enteritidis originated from layer and broiler farms in Java Island |
| title_sort | detection of salmonella pathogenicity island and salmonella plasmid virulence genes in salmonella enteritidis originated from layer and broiler farms in java island |
| topic | Java Island; S. Enteritidis; virulence genes; SPIs; virulence plasmids |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=51029 |
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